Imprecise excision of P{lacW}SmoxG0348 resulting in a deletion that removes a portion of the 5' UTR of Smox (breakpoint at nucleotide 457 of sequence AF101386) and the entire coding region. The 3' breakpoint is found to be 275 bp downstream of the Smox transcribed region and the deletion does not disrupt the neighbouring gene. Several segments of the original P-element are left behind.
Inferred boundaries of a deletion resulting from the imprecise excision of P{lacW}SmoxG0348, which takes out the entire Smox coding region. It is reported as extending from base 457 of GB:AF101386 to 275 bp downstream of the Smox transcribed region. The 3' end of Smox-RA and GB:AF101386 were used as the end of the transcription unit for the mapping.
fertile (with Dp(1;3)DC185)
fertile (with Dp(1;3)DC186)
viable (with Dp(1;3)DC185)
viable (with Dp(1;3)DC186)
SmoxF4 homozygous clonal wings and legs within SmoxF4-heterozygous individuals (i.e. whole tissue somatic clone) are smaller than controls; the mutant wings develop ectopic veins around the longitudinal L5 vein.
SmoxF4 homozygous wing disc clones within an heterozygous tissue are significantly smaller than their control wild-type twin clones.
Unlike in wild type, whose dendrites cluster around the photoreceptor axons in their cognate column, SmoxF4 mutant Tm20 neuron clones extend their neurons laterally in medulla layer M3a and invade neighboring columns. Furthermore, the mutant dendritic branches in the internal medulla M8 layer exhibit a similar overextension phenotype. Total segment number is reduced and average segment length is increased. Dendritic termination frequency is reduced.
The dendrites of SmoxF4 mutant Dm8 neuron clones occupy a larger space than wild type ones. SmoxF4 mutant Dm8 dendrites occupy significantly more dendritic field units than the wild type ones.
SmoxF4 mutant larvae maintain normal anal pad structures. Hypotrophy of the central nervous system is seen in late third instar larvae and wing discs are drastically widened compared to controls. The wing disc overgrowth is restricted to the anterior-posterior axis of the pouch and hinge portion of the disc. The long proximal-distal axis is neither shortened nor lengthened, and the notum part of the disc is not expanded. The epithelial layers remain intact and the pattern of epithelial folding is similar to normal discs. Although SmoxF4 mutant wing discs are enlarged, the wing blade cells are smaller, meaning that approximately 1.8 times the number of cells are found in SmoxF4 mutant discs compared to controls. SmoxF4 mutant wing discs are a normal size and shape at the beginning of the third instar larval stage (L3), but by mid L3 widening has taken place. As in wild type the discs continue to grow through the remainder of L3, but the width-height ratio is not altered an further, remaining the same as during mid L3.
The total number of mitotic cells in SmoxF4 mutant wing discs is reduced and, unlike the uniform pattern seen in wild type, a marked asymmetry is seen along the A/P axis, with the lateral regions containing far more mitotic cells than the medial region. At early L3 (when wing discs are normal in size and shape) the pattern of mitosis appears normal. At mid L3 (when widening is apparent), asymmetry is observed and the total number of mitotic cells is modestly reduced compared to wild type discs.
Hemizygous SmoxF4 males die as larvae or pupae. SmoxF4 is fully rescued to viability by and fertility by one copy of either Dp(1;3)DC185 or Dp(1;3)DC186.
SmoxF4 has increased size | larval stage phenotype, suppressible by myoRNAi.2259-3503.UAS/Scer\GAL4da.G32
SmoxF4 has increased size | larval stage phenotype, suppressible by Scer\GAL4Mhc.PK/myoRNAi.2259-3503.UAS
SmoxF4 has increased size | larval stage phenotype, suppressible by myoRNAi.2259-3503.UAS/Scer\GAL4Mef2.PR
SmoxF4 has abnormal size | late third instar larval stage phenotype, suppressible by babo4
SmoxF4 has increased size | larval stage phenotype, non-suppressible by myoRNAi.2259-3503.UAS/Scer\GAL4NP5130
SmoxF4 has increased size | larval stage phenotype, non-suppressible by myoRNAi.2259-3503.UAS/Scer\GAL4repo
SmoxF4, myoΔ1 has decreased size | larval stage phenotype
SmoxF4 has wing disc | larval stage phenotype, suppressible by Scer\GAL4Mhc.PK/myoRNAi.2259-3503.UAS
SmoxF4 has wing disc | larval stage phenotype, suppressible by myoRNAi.2259-3503.UAS/Scer\GAL4Mef2.PR
SmoxF4 has wing disc | larval stage phenotype, suppressible by myoRNAi.2259-3503.UAS/Scer\GAL4da.G32
SmoxF4 has wing disc | late third instar larval stage phenotype, suppressible by babo4
SmoxF4 has wing disc | larval stage phenotype, non-suppressible by myoRNAi.2259-3503.UAS/Scer\GAL4NP5130
SmoxF4 has wing disc | larval stage phenotype, non-suppressible by myoRNAi.2259-3503.UAS/Scer\GAL4repo
SmoxF4, myoΔ1 has wing disc | larval stage phenotype
SmoxF4 is rescued by Scer\GAL4da.G32/SmoxUAS.cPa.Tag:FLAG
SmoxF4 is rescued by SmoxAAMA.UAS.Tag:FLAG/Scer\GAL4da.G32
SmoxF4 is not rescued by SmoxRB4.UAS.Tag:FLAG/Scer\GAL4da.G32
Expression of SmoxScer\UAS.attB.T:Zzzz\FLAG under the control of Scer\GAL4da.G32 rescues the wing disc widening phenotype seen in SmoxF4 mutant third instar larvae.
Expression of SmoxAAMA.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4da.G32 rescues the wing disc widening phenotype seen in SmoxF4 mutant third instar larvae.
Expression of SmoxRB4.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4da.G32 fails to rescue the wing disc widening phenotype seen in SmoxF4 mutant third instar larvae.
