FB2024_03 , released June 25, 2024
Allele: Ecol\lexAGMR71G01
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General Information
Symbol
Ecol\lexAGMR71G01
Species
E. coli
Name
FlyBase ID
FBal0277015
Feature type
allele
Associated gene
Ecol\lexA
Associated Insertion(s)
Carried in Construct
P{GMR71G01-lexA}
Also Known As
R71G01-LexA
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Pfeiffer et al., 2013.10.9, Pan et al., 2012)
Progenitor genotype
Carried in construct
P{GMR71G01-lexA}
Cytology
Description

A 3844 base pair fragment from the flanking non-coding or intronic region of Vsx2 is fused upstream of a Drosophila core synthetic promoter (DSCP) followed by sequence encoding a lexA::p65 driver. The driver sequence has been optimized for Drosophila codon usage. An Hsp70 transcriptional terminator is present.

(Pfeiffer et al., 2013.10.9)
Allele components
Component
Use(s)
Regulatory region(s)
GMR71G01
Encoded product / tool
lexA::p65
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      neuron | subset of adult brain
      (Pan et al., 2012)
      neuron | subset of adult ventral nerve cord
      (Pan et al., 2012)
      adult fruitless pMP-e (female) neuron | subset
      (Pan et al., 2012)
      adult doublesex pC1 (female) neuron
      (Zhou et al., 2015)
      adult doublesex pC1 (male) neuron
      (Zhou et al., 2015)
      adult fruitless P1 (male) neuron

      Comment: A FLP/FRT strategy shows P{GMR71G01-lexA} drives expression in adult ingestion neuron 1 when combined with TI{GAL4::p65}CCKLR-17D3GAL4::p65.

      (Wu et al., 2019)
      adult stage | male
      adult doublesex pC1 (male) neuron | subset
      (Zhou et al., 2014)
      adult fruitless P1 (male) neuron | subset
      (Hoopfer et al., 2015)
      Additional Information
      Statement
      Reference

      A FLP/FRT intersectional strategy shows expression of EcollexAGMR71G01 in adult doublesex pC1 (female and male) neurons when combined with ScerGAL4dsx.Δ2.

      (Zhou et al., 2015)

      Expression driven by EcollexAGMR71G01 was restricted to a subset of adult fruitless pMP-e (male) neurons when combined with ScerGAL4dsx.Δ2 in a FLP based intersection strategy.

      (Hoopfer et al., 2015)

      EcollexAGMR71G01 drives expression in a subset of adult doublesex pC1 (male) neuron that also have expression driven by EcollexAfru.P1.

      (Zhou et al., 2014)

      EcollexAR71G01 drives expression in a subset of neurons in the adult CNS, including the male-specific P1 (pMP-e) neurons in the brain. The P1 neurons overlap with the expression of ScerGAL4fru.P1.B or ScerGAL4dsx-GAL4 in males. A small number of abdominal ganglion neurons and glial cells are also detected when expressing both EcollexAR71G01 and ScerGAL4dsx-GAL4.

      (Pan et al., 2012)
       
      Marker for
      Reflects expression of
      dsx
      (Zhou et al., 2014)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Thermogenetic activation (using TrpA1FRT.stop.UAS.Tag:MYC at 29[o]C with Scer\FLP1L.8xlexAop) of cells expressing Scer\GAL4dsx.Δ2 and Ecol\lexAGMR71G01 in adult males significantly increases aggressive lunging behavior and wing extensions towards other males, compared to controls.

      (Hoopfer et al., 2015)

      Inhibition (using Ctet\TeTxLCFRT.stop.UAS with Scer\FLP1L.8xlexAop) of neurons expressing Ecol\lexAGMR71G01 and Scer\GAL4dsx.Δ2 significantly reduces male-male courtship chaining responses to pulse song, compared to controls.

      Optogenetic activation (using Cnoc\ChR1Cs.20xUAS.FRT.Venus with Scer\FLP1L.8xlexAop and activating with 655 nm (red) light) of neurons expressing Ecol\lexAGMR71G01 and Scer\GAL4dsx.Δ2 significantly increases male-male courtship chaining, compared to controls.

      (Zhou et al., 2015)

      Inhibition (using Ctet\tetXTNT-LC.FRT.stop.UAS with Scer\FLP1L.8xlexAop) of cells expressing both Scer\GAL4dsx.Δ2 and Ecol\lexAGMR71G01 in adult virgin females significantly reduces copulation within 10 min of introduction to a male, compared to controls.

      Thermogenetic activation (using TrpA1FRT.stop.UAS.Tag:MYC with Scer\FLP1L.8xlexAop at 27[o]C) of cells expressing both Scer\GAL4dsx.Δ2 and Ecol\lexAGMR71G01 in mature adult virgin females, but not immature or mated females, significantly increases copulation within 10 min of introduction to a male, compared to baseline, unlike controls.

      (Zhou et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Ecol\lexAGMR71G01
      Ecol\lexAR71G01
      Name Synonyms
      Secondary FlyBase IDs
        References (19)