Contains a genomic fragment centred on RabX4 in which the complete RabX4 open reading frame has been replaced with the GAL4 driver coding region and a loxP cassette containing Disc\RFP3xP3.PC and the kanamycin resistance gene.
ScerGAL4RabX4.ORF drives expression in almost all neurons and possibly glia in the larval, pupal and adult brain. Expression is also detected in the developing photoreceptors in the larval eye disc and in the adult photoreceptor cells. Expression in the adult mushroom body is stronger than in the pupa.
The PBac{I-SceI(FRT.RabX4-GAL4.ORF(loxP.3xP3-RFP))} vector can be used both as a driver expressing Scer\GAL4 under the control of the RabX4 regulatory sequences present in the construct and also as a donor construct for ends-out homologous recombination at the endogenous RabX4 locus.