Contains a genomic fragment centred on Rab6 in which the complete Rab6 open reading frame has been replaced with the GAL4 driver coding region and a loxP cassette containing Disc\RFP3xP3.PC and the kanamycin resistance gene.
ScerGAL4Rab6.ORF drives expression in the larval brain neurons, as well as in the eye, wing and leg imaginal discs. In the pupal and adult brain, expression is ubiquitous. Expression in the adult mushroom body is stronger than in the pupal one.
The PBac{I-SceI(FRT.Rab6-GAL4.ORF(loxP.3xP3-RFP))} vector can be used both as a driver expressing Scer\GAL4 under the control of the Rab6 regulatory sequences present in the construct and also as a donor construct for ends-out homologous recombination at the endogenous Rab6 locus.