FB2024_03 , released June 25, 2024
Allele: Scer\GAL4Rab30.ORF
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General Information
Symbol
Scer\GAL4Rab30.ORF
Species
S. cerevisiae
Name
FlyBase ID
FBal0283524
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
PBac{I-SceI(FRT.Rab30-GAL4.ORF(loxP.3xP3-RFP))}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Jin et al., 2012)
Progenitor genotype
Carried in construct
PBac{I-SceI(FRT.Rab30-GAL4.ORF(loxP.3xP3-RFP))}
Cytology
Description

Contains a genomic fragment centred on Rab30 in which the complete Rab30 open reading frame has been replaced with the GAL4 coding region and a loxP cassette containing Disc\RFP3xP3.PC and the kanamycin resistance gene.

(Jin et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
Rab30
Encoded product / tool
GAL4
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage
      neuron of embryonic/larval brain
      (Jin et al., 2012)
      embryonic/larval brain | restricted
      (Jin et al., 2012)
      embryonic/larval salivary gland
      (Jin et al., 2012)
      neuron | subset of larval ventral nerve cord
      (Jin et al., 2012)
      neuropil glial sheath | faint
      (Jin et al., 2012)
      Bolwig organ | restricted
      (Jin et al., 2012)
      embryonic/larval mushroom body | faint
      (Jin et al., 2012)
      eye disc | restricted
      (Jin et al., 2012)
      embryonic/larval optic stalk sheath cell
      (Jin et al., 2012)
      leg disc | restricted
      (Jin et al., 2012)
      wing disc | faint | restricted
      (Jin et al., 2012)
      pupal stage P6 -- P7
      eye photoreceptor cell

      Comment: 30-40h APF

      (Jin et al., 2012)
      pupal stage P7
      neuron of pupal brain
      (Jin et al., 2012)
      adult stage
      neuron of adult brain
      (Jin et al., 2012)
      adult brain | restricted
      (Jin et al., 2012)
      adult ensheathing glial cell | faint
      (Jin et al., 2012)
      neuron of adult optic lobe
      (Jin et al., 2012)
      adult antennal lobe
      (Jin et al., 2012)
      adult insulin secreting cell of pars intercerebralis
      (Jin et al., 2012)
      ellipsoid body | faint
      (Jin et al., 2012)
      fan-shaped body | faint
      (Jin et al., 2012)
      Additional Information
      Statement
      Reference

      ScerGAL4Rab30.ORF drives expression in the larval brain neurons, as well as in the eye, wing and leg imaginal discs. In the adult brain, expression is ubiquitous, with expression levels varying in different cell types.

      (Jin et al., 2012)
       
      Marker for
      Reflects expression of
      Rab30
      (Jin et al., 2012)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      The PBac{I-SceI(FRT.Rab30-GAL4.ORF(loxP.3xP3-RFP))} vector can be used both as a driver expressing Scer\GAL4 under the control of the Rab30 regulatory sequences present in the construct and also as a donor construct for ends-out homologous recombination at the endogenous Rab30 locus.

      (Jin et al., 2012)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4Rab30.ORF
      Name Synonyms
      Secondary FlyBase IDs
        References (2)