Reintegration of the 19.3 kb fragment removed in Abd-Biab-5.6.CI carrying an approximately 2.9 kb internal deletion spanning Drosophila genome project release 5 coordinates 3R:12721546-12724398 .
Abd-Biab-6.cocu mutant flies show distinct morphological abnormalities in the secondary cells of the male accessory gland. The mutant cells lack the large vacuolar structures seen in wild type.
When Abd-Biab-6.cocu mutant males are crossed to wild-type females the number of eggs produced is initially comparable to controls. However, at 48 hours the number of eggs produced is decreased dramatically and the total number of eggs laid over a 10 day period is significantly lower than when females are mated with wild-type males. The proportion of progeny that eclose is similar to wild type, indicating that hatchability is not affected.
Sexual receptivity is initially repressed in mates of Abd-Biab-6.cocu males, but this effect is not maintained; as with wild type males, females that have been mated with Abd-Biab-6.cocu males are unreceptive to remating with wild type males at 24 hours, but at 4 days females are more receptive to remating. By 10 days, both groups are fully receptive.
When wild type females are first mated with a Abd-Biab-6.cocu mutant male and then allowed to mate for a second time with a wild type male, the proportion of Abd-Biab-6.cocu mutant progeny is higher than the proportion of wild type progeny seen when the female first mates with the wild type and then with the Abd-Biab-6.cocu mutant male.
Females mated to either wild type or Abd-Biab-6.cocu mutant males store comparable amounts of sperm in the seminal receptacle at both two hours and four days after mating. By 10 days the females mated to Abd-Biab-6.cocu males show a slight but significant decrease in stored sperm. The amount of sperm stored in the spermathecae is comparable to wild type at all time points.
Abd-Biab-6.Δ5 mutants show no dramatical abnormalities.