Flies expressing Avic\GFP^{EGFP.Scer\UAS-mir-276aSP} under the control of either Scer\GAL4^elav.PU or Scer\GAL4^c547 have a significantly defective naive odour avoidance response to 4-methylcyclohexanol (1 x 10[-3] v/v) compared to wild type.

Flies expressing Avic\GFP^{EGFP.Scer\UAS-mir-276aSP} under the control of either Scer\GAL4^Orco.PU, Scer\GAL4^GH146, Scer\GAL4^GH298, Scer\GAL4^c747 or Scer\GAL4^Aph-4-c232 have a normal naive odour avoidance response to 4-methylcyclohexanol (1 x 10[-3] v/v).

Flies expressing Avic\GFP^{EGFP.Scer\UAS-mir-276aSP} under the control of Scer\GAL4^elav.PU and raised at 29[o]C (to inactivate a Scer\GAL80^ts.αTub84B allele which is present) show a significant impairment in naive odour avoidance response to 4-methylcyclohexanol when they are maintained at 29[o]C after eclosion. If the flies are shifted to 18[o]C after eclosion (resulting in repression of Scer\GAL4^elav.PU by Scer\GAL80^ts.αTub84B) a significant restoration of naive odour avoidance response to 4-methylcyclohexanol is seen.

Flies expressing Avic\GFP^{EGFP.Scer\UAS-mir-276aSP} under the control of Scer\GAL4^ey-OK107 have a normal naive odour avoidance response to 4-methylcyclohexanol (1 x 10[-3] v/v), 3-octanol (1.5 x 10[-3] v/v), and to benzaldehyde (0.5 x 10[-3] v/v). The flies also show a normal avoidance response to electric shock (60V).

Flies expressing Avic\GFP^{EGFP.Scer\UAS-mir-276aSP} under the control of Scer\GAL4^ey-OK107 show significantly reduced long term olfactory memory (using 3-octanol and benzaldehyde as the odours) measured 24 hours after spaced repetitive training, but memory measured 24 hours after repetitive massed training or short-term memory measured immediately after one training session is normal.

Flies expressing Avic\GFP^{EGFP.Scer\UAS-mir-276aSP} under the control of Scer\GAL4^c747 show significantly reduced long-term olfactory memory measured 24 hours after spaced repetitive training.

Flies expressing Avic\GFP^{EGFP.Scer\UAS-mir-276aSP} under the control of Scer\GAL4^ey-OK107 and raised at 29[o]C (to inactivate a Scer\GAL80^ts.αTub84B allele which is present) show a significant impairment in long-term olfactory memory measured 24 hours after spaced repetitive training when they are maintained at 29[o]C after eclosion. If the flies are shifted to 18[o]C after eclosion (resulting in repression of Scer\GAL4^ey-OK107 by Scer\GAL80^ts.αTub84B) full restoration of long-term olfactory memory measured 24 hours after spaced repetitive training is seen.

Flies expressing mir-276a^{sponge.Scer\UAS.T:Avic\GFP-EGFP} under the control of either Scer\GAL4^elav.PU or Scer\GAL4^c547 have a significantly defective naive odour avoidance response to 4-methylcyclohexanol (1 x 10[-3] v/v) compared to wild type.

Flies expressing mir-276a^{sponge.Scer\UAS.T:Avic\GFP-EGFP} under the control of either Scer\GAL4^Orco.PU, Scer\GAL4^GH146, Scer\GAL4^GH298, Scer\GAL4^c747 or Scer\GAL4^Aph-4-c232 have a normal naive odour avoidance response to 4-methylcyclohexanol (1 x 10[-3] v/v).

Flies expressing mir-276a^{sponge.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^elav.PU and raised at 29[o]C (to inactivate a Scer\GAL80^ts.αTub84B allele which is present) show a significant impairment in naive odour avoidance response to 4-methylcyclohexanol when they are maintained at 29[o]C after eclosion. If the flies are shifted to 18[o]C after eclosion (resulting in repression of Scer\GAL4^elav.PU by Scer\GAL80^ts.αTub84B) a significant restoration of naive odour avoidance response to 4-methylcyclohexanol is seen.

Flies expressing mir-276a^{sponge.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^ey-OK107 have a normal naive odour avoidance response to 4-methylcyclohexanol (1 x 10[-3] v/v), 3-octanol (1.5 x 10[-3] v/v), and to benzaldehyde (0.5 x 10[-3] v/v). The flies also show a normal avoidance response to electric shock (60V).

Flies expressing mir-276a^{sponge.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^ey-OK107 show significantly reduced long term olfactory memory (using 3-octanol and benzaldehyde as the odours) measured 24 hours after spaced repetitive training, but memory measured 24 hours after repetitive massed training or short-term memory measured immediately after one training session is normal.

Flies expressing mir-276a^{sponge.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^c747 show significantly reduced long-term olfactory memory measured 24 hours after spaced repetitive training.

Flies expressing mir-276a^{sponge.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^ey-OK107 and raised at 29[o]C (to inactivate a Scer\GAL80^ts.αTub84B allele which is present) show a significant impairment in long-term olfactory memory measured 24 hours after spaced repetitive training when they are maintained at 29[o]C after eclosion. If the flies are shifted to 18[o]C after eclosion (resulting in repression of Scer\GAL4^ey-OK107 by Scer\GAL80^ts.αTub84B) full restoration of long-term olfactory memory measured 24 hours after spaced repetitive training is seen.

Expression of Avic\GFP^{EGFP.Scer\UAS-mir-276aSP} under the control of Scer\GAL4^how-24B does not result in any detectable neuromuscular junction defects.

Expression of mir-276a^{sponge.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^how-24B does not result in any detectable neuromuscular junction defects.