Imprecise excision of the P{GawB} element in P{GawB}Bxhdp-G14-1 has resulted in a ~2kb deletion from the Bx gene sequence.
1895 bp deletion resulting from the imprecise excision of P{GawB}Bxhdp-G14-1, which removes the first three coding exons of Bx. Deletion boundaries based on reported flanking sequences.
Bx7 females exhibit normal stages of ovariole development with proper border cell migration in egg chambers as in controls.
Some of the eggs laid by Bx7 females show defects: the dorsal appendages are thin and wavy; the distance between the bases of dorsal appendages is wider; and the egg shells are translucent when compared to the controls.
Bx7 mutant flies have a sever held-up wings phenotype.
Bx7 mutant females show normal receptivity and normal mating duration. Despite showing normal mating duration, Bx7 mutant females show highly reduced fecundity and fertility compared to wild type. Unhatched eggs are white in color, indicating a defect in fertilization. Oviposition defects are also seen: in contrast to control flies, Bx7 mutant females lay eggs on the surface of the media rather than in it.
The ovaries of Bx7 mutant females contain fewer ovarioles than controls, but individual ovarioles develop normally. Ovulation is reduced compared to controls. Only 10% of eggs laid by Bx7 mutant females hatch.
Bx7 mutant females have normal sperm storing ability, but in contrast to wild type flies, they do not release the sperm for fertilisation. At 10 days post-mating the seminal receptacle of Bx7 mutant still retains sperm.
