FB2024_03 , released June 25, 2024
Allele: Scer\GAL4VP16.nanos.FRT.STOP
Open Close
General Information
Symbol
Scer\GAL4VP16.nanos.FRT.STOP
Species
S. cerevisiae
Name
FlyBase ID
FBal0301400
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
M{nanos(FRT.STOP)GAL4::VP16}
Also Known As
nos-FRT-stop-FRT-Gal4, nos>stop>Gal4, nos > stop > gal4
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Ma et al., 2014, Salzmann et al., 2013)
Progenitor genotype
Carried in construct
M{nanos(FRT.STOP)GAL4::VP16}
Cytology
Description

The nos promoter is separated from a GAL4::VP16 driver by an FRT cassette which contains the mCherry coding region followed by the SV40 polyA signal. The nos 3'UTR is present downstream of the GAL4::VP16 coding sequence.

(Ma et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
nanos
Encoded product / tool
GAL4::VP16
Also carries
FRT
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference

      Designed for use in mosaic analyses; transcription blocked by FRT-flanked fragment containing mCherry coding region followed by the SV40 polyA signal. See expression pattern for ScerGAL4VP16.nos.-FRT, carried in descendant transposon M{nos(-FRT)GAL4::VP16}.

      (Salzmann et al., 2013)
       
      Marker for
      Reflects expression of
      nanos
      (Salzmann et al., 2013)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Scer\GAL4VP16.nanos.FRT.STOP
      Scer\GAL4VP16.nos.FRT.STOP
      Scer\GAL4nos.Scer\FRT.STOP.T:Hsim\VP16
      Scer\GAL4nos.Scer\FRT.STOP
      Name Synonyms
      Secondary FlyBase IDs
        References (12)