Mutation in sequenced strain: frameshift (-2).
There is a 2 base deletion in Cyp6a14 in iso-1, the reference sequence strain relative to RNA-seq reads from a different melanogaster strain (SRX029401). Two bases (GT) are missing between 2R:8565716 and 2R:8565717 in iso-1. This leads to a frameshift after codon 73 and early translation termination.