The R85D07 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.
Comment: when combined with P{R13F02-p65.AD} (combination referred to as 'MB371B')
when combined with P{R13F02-p65.AD} (combination referred to as 'MB371C')
Blocking synaptic transmission (using shits1.IVS.p10.20xScer\UAS at 33[o]C) from neurons targeted by the split-GAL4 combination of Hsap\RELAAD.R13F02 and Scer\GAL4DBD.R85D07 does not significantly impair visual (aversive color discrimination conditioning) memory performance, compared to controls.
Thermogenetic activation (using TrpA1UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R85D07 and Hsap\RELAAD.R13F02 in adult males or females does not significantly affect the amount of sleep, compared to controls; activation does not significantly affect locomotor activity of awake adult males, compared to controls.
Split-GAL4 transgene, constructed as described in FBrf0212052.