FB2024_03 , released June 25, 2024
Allele: Dmel\dncMI03415-GFSTF.2
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General Information
Symbol
Dmel\dncMI03415-GFSTF.2
Species
D. melanogaster
Name
FlyBase ID
FBal0304671
Feature type
allele
Associated gene
Dmel\dnc
Associated Insertion(s)
Mi{PT-GFSTF.2}dncMI03415-GFSTF.2
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
phiC31 integrase
(Gene Disruption Project members, 2015-)
Progenitor genotype
Mi{MIC}dncMI03415
(Gene Disruption Project members, 2015-)
Associated Insertion(s)
Mi{PT-GFSTF.2}dncMI03415-GFSTF.2
Cytology
Description

The RMCE cassette of the Mi{MIC}dncMI03415 insertion has been replaced with a EGFP-FlAsH-StrepII-TEV-3xFlag multi-tag cassette. The insertion is in a coding intron of dnc and is predicted to protein trap the gene. The insertion is also within CR44886, not in a coding intron, and is not predicted to protein trap this gene.

(Gene Disruption Project members, 2015-)
Allele components
Component
Use(s)
Inserted element
Mi{PT-GFSTF.2}
Tagged with
EGFP
Tag:CALI(TC)
Tag:CS(TEVp)
Tag:FLAG
Tag:StrepII
Mutations Mapped to the Genome
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      dncMI03415-GFSTF.2 adults in which the level of dnc protein has been reduced by expressing slmbScer\UAS.T:Zzzz\vhhGFP4 (which targets GFP-tagged proteins for degradation) under the control of Scer\GAL4117Y using a 3-day temperature shift to 28[o]C starting at 1 day after eclosion show a significant reduction in performance index in an aversive olfactory learning assay compared to control flies. This learning defect is fully rescued after 48 hours at 18[o]C (which allows the levels of dnc protein to be re-established).

      (Nagarkar-Jaiswal et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Dnc-EGFP-Dnc
      (Nagarkar-Jaiswal et al., 2015)
      dncMI03415-GFSTF.2
      Name Synonyms
      Secondary FlyBase IDs
        References (4)