The R22D06 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.
Comment: when combined with P{VT056582-p65.AD} (combination referred to as 'SS01052')
Comment: when combined with P{R30C12-p65.AD} (combination referred to as 'SS01066')
Comment: when combined with P{R35D04-p65.AD} (combination referred to as 'OL0019B')
Inhibition (using Ctet\tetXTNT-LC.UAS) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R22D06 and Hsap\RELAAD.R35D04 in adult males does not affect their ability to orient towards and remain close to a female, compared to controls; there is also no effect on following of females or wing extensions, compared to controls.
Split-GAL4 transgene, constructed as described in FBrf0212052.