FB2024_03 , released June 25, 2024
Allele: Scer\GAL4DBD.R34A03
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General Information
Symbol
Scer\GAL4DBD.R34A03
Species
S. cerevisiae
Name
FlyBase ID
FBal0326966
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{R34A03-GAL4.DBD}
PBac{R34A03-GAL4.DBD}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Aso et al., 2014, Bräcker et al., 2013)
Progenitor genotype
Carried in construct
P{R34A03-GAL4.DBD}
PBac{R34A03-GAL4.DBD}
Cytology
Description

The R34A03 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
GMR34A03
Encoded product / tool
GAL4(DBD)::Zip-
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      alpha'/beta' Kenyon cell

      Comment: when combined with P{R52H09-p65.AD} (combination referred to as 'MB186B')

      when combined with P{R13F02-p65.AD} (combination referred to as 'MB005B')

      (Aso et al., 2014, Vogt et al., 2014)
      alpha'/beta' anterior-posterior Kenyon cell | intense

      Comment: when combined with P{R35B12-p65.AD} (combination referred to as 'MB463B')

      (Aso et al., 2014)
      alpha'/beta' middle Kenyon cell

      Comment: when combined with P{R35B12-p65.AD} (combination referred to as 'MB463B')

      when combined with P{R13F02-p65.AD} (combination referred to as 'MB005B')

      (Vogt et al., 2016, Aso et al., 2014)
      alpha'/beta' anterior-posterior Kenyon cell

      Comment: when combined with P{R13F02-p65.AD} (combination referred to as 'MB005B')

      (Vogt et al., 2016)
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Adult males with inhibition (using Ctet\tetXTNT-LC.UAS) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R34A03 and Hsap\RELAAD.R13F02 have significantly weaker suppression of courtship behavior following training with mated females, compared to controls.

      (Zhao et al., 2018)

      Blocking synaptic transmission (using shits1.IVS.p10.20xScer\UAS at 33[o]C) from neurons targeted by the split-GAL4 combination of Hsap\RELAAD.R13F02 and Scer\GAL4DBD.R34A03 does not significantly impair visual (aversive color discrimination conditioning) memory performance, compared to controls.

      (Vogt et al., 2016)

      Thermogenetic activation (using TrpA1UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pairs of Scer\GAL4DBD.R34A03 and Hsap\RELAAD.R52H09, or Scer\GAL4DBD.R34A03 and Hsap\RELAAD.R19B03, in adult males or females significantly decreases the amount of sleep, compared to controls; activation of neurons targeted by Scer\GAL4DBD.R34A03 and Hsap\RELAAD.R13F02, or Scer\GAL4DBD.R34A03 and Hsap\RELAAD.R35B12, in adult males, but not females, significantly decreases the amount of sleep, compared to controls; in the case of Scer\GAL4DBD.R34A03 and Hsap\RELAAD.R35B12, but not Scer\GAL4DBD.R34A03 and Hsap\RELAAD.R52H09, Scer\GAL4DBD.R34A03 and Hsap\RELAAD.R19B03, or Scer\GAL4DBD.R34A03 and Hsap\RELAAD.R13F02, activation significantly decreases locomotor activity of awake adult males, compared to controls.

      (Sitaraman et al., 2015)

      Inhibition (using shi1.UAS at 33[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R34A03 and Hsap\RELAAD.R52H09 in adults does not significantly affect aversive or appetitive 1-min olfactory memory, compared to controls.

      (Vogt et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (5)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Split-GAL4 transgene, constructed as described in FBrf0212052.

      (Aso et al., 2014)

      "Split GAL4" system transgene.

      (Bräcker et al., 2013)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Scer\GAL4DBD.34A03.T:Zzzz\Zip-
      Scer\GAL4DBD.34A03.T:Zzzz\ZipRREEL
      Scer\GAL4DBD.R34A03.T:Zzzz\Zip-
      Scer\GAL4DBD.R34A03
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0304454
      • FBal0290916
      References (27)