FB2024_03 , released June 25, 2024
Allele: Dmel\Mfap1DsRed
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General Information
Symbol
Dmel\Mfap1DsRed
Species
D. melanogaster
Name
FlyBase ID
FBal0328049
Feature type
allele
Associated gene
Dmel\Mfap1
Associated Insertion(s)
TI{TI}Mfap1DsRed
Carried in Construct
Key Links
Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Rode et al., 2017)
Mutagen
CRISPR/Cas9
(Rode et al., 2017)
gene targeting by homologous recombination
(Rode et al., 2017)
Progenitor genotype
Associated Insertion(s)
TI{TI}Mfap1DsRed
Cytology
Description

A cassette containing a screenable eye marker (Disc\RFPDsRed.3xP3.cUa) is inserted info the last coding exon of Mfap1, around 100bp in front of the stop codon and resulting in disruption of the conserved C-terminal domain.

(Rode et al., 2017)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhancer of
      Statement
      Reference

      Mfap1[+]/Mfap1DsRed is a non-enhancer of abnormal neuroanatomy | pupal stage phenotype of Scer\GAL4ppk.PG, TER94E375Q.E575Q.UAS

      (Rode et al., 2017)
      Phenotype Manifest In
      NOT Enhancer of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The dendrite pruning defects in class IV dendrite arborizing neurons observed in pupae expressing TER94E375Q.E575Q.Scer\UAS under the control of Scer\GAL4ppk.PG is not significantly affected by combination with a single copy of Mfap1DsRed.

      (Rode et al., 2017)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Mfap1DsRed/Df(3L)ED4287 is rescued by Mfap1UAS.GFP

      (Rode et al., 2017)

      Mfap1DsRed/Df(3L)ED4287 is rescued by Scer\GAL4Act.PU/Mfap1230-478.UAS.GFP

      (Rode et al., 2017)
      Not rescued by

      Mfap1DsRed/Df(3L)ED4287 is not rescued by Scer\GAL4Act.PU/Mfap11-229.UAS.GFP

      (Rode et al., 2017)
      Comments

      The lethality of Mfap1DsRed/Df(3L)ED4287 animals is rescued by expression of Mfap1230-478.Scer\UAS.T:Avic\GFP (but not Mfap11-229.Scer\UAS.T:Avic\GFP) under the control of Scer\GAL4Act.PU driver or by leak expression of Mfap1Scer\UAS.T:Avic\GFP, i.e. without any Gal4 driver (overexpression of Mfap1Scer\UAS.T:Avic\GFP under the Scer\GAL4Act.PU driver is lethal).

      (Rode et al., 2017)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Mfap1DsRed
      mfap1DsRed
      (Rode et al., 2017)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)