FB2024_03 , released June 25, 2024
Allele: Scer\GAL4DBD.R60D05
Open Close
General Information
Symbol
Scer\GAL4DBD.R60D05
Species
S. cerevisiae
Name
FlyBase ID
FBal0332174
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{R60D05-GAL4.DBD}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Rubin Laboratory at Janelia Farm, 2017)
Progenitor genotype
Carried in construct
P{R60D05-GAL4.DBD}
Cytology
Description

The GMR60D05 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).

(Rubin Laboratory at Janelia Farm, 2017)
Allele components
Component
Use(s)
Regulatory region(s)
GMR60D05
Encoded product / tool
GAL4(DBD)::Zip-
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      adult ellipsoid body-protocerebral bridge 1 glomerulus-dorsal gall neuron

      Comment: when combined with P{R27F02-p65.AD} (combination referred to as 'SS00131')

      (Giraldo et al., 2018)
      adult ellipsoid body-protocerebral bridge 1 glomerulus-ventral gall neuron

      Comment: when combined with P{R27F02-p65.AD} (combination referred to as 'SS00131')

      (Giraldo et al., 2018)
      adult ellipsoid body-protocerebral bridge 1 glomerulus-dorsal/ventral gall neuron

      Comment: when combined with P{R27F02-p65.AD} (combination referred to as 'SS00131')

      (Turner-Evans et al., 2020)
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Inhibition (using Hsap\KCNJ2IVS.10xUAS.EGFP) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R60D05 and Hsap\RELAAD.R27F02 in adults results in a reduction in the ability to maintain a light stimulus at an arbitrary azimuthal position during flight, compared to controls, but the ability to fixate on light stimuli frontally is maintained.

      (Giraldo et al., 2018)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Scer\GAL4DBD.R60D05.T:Zzzz\Zip-
      Scer\GAL4DBD.R60D05
      Name Synonyms
      Secondary FlyBase IDs
        References (4)