FB2024_02 , released April 23, 2024
Allele: Dmel\SNF4AγMI09417-FF.PT-EGFP
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General Information
Symbol
Dmel\SNF4AγMI09417-FF.PT-EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0335430
Feature type
allele
Associated gene
Dmel\SNF4Aγ
Associated Insertion(s)
Mi{FlipFlop.1}SNF4AγMI09417-FF.PT-EGFP
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
phiC31 integrase
(Nagarkar-Jaiswal et al., 2017)
Progenitor genotype
Mi{MIC}SNF4AγMI09417
(Nagarkar-Jaiswal et al., 2017)
Associated Insertion(s)
Mi{FlipFlop.1}SNF4AγMI09417-FF.PT-EGFP
Cytology
Description

The RMCE cassette of the Mi{MIC}SNF4AγMI09417 insertion has been replaced with a FlipFlop cassette in protein-trap (PT) orientation relative to SNF4Aγ. This results in the SNF4Aγ protein being tagged with EGFP.

(Nagarkar-Jaiswal et al., 2017)
Allele components
Component
Use(s)
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Scer\FLP1ey.PU;SNF4AγMI09417-FF.PT-EGFP/Df(3R)e-R1 adult flies (using the Scer\FLP1ey.PU flippase to inactivate expression from the SNF4AγMI09417-FF.PT-EGFP allele by inverting the 'flip-flop' insertion it contains) show significantly reduced amplitude in electroretinogram readings as compared to age-matched controls (same level of reduction is observed both in young and aged mutant flies) as well as decreased number of rhabdomeres and defects in photoreceptor organization.

      Heat-shock induced (by using the Scer\FLP1hs.PU) inactivation of the SNF4AγMI09417-FF.PT-EGFP allele in SNF4AγMI09417-FF.PT-EGFP/SNF4AγMI09417 mutant flies at 3 days post eclosion leads to severe neurodegeneration with multiple lesions in the brain (the neurodegeneration doesn't appear to progress further as the flies age but they typically die 3-4 weeks later).

      Scer\FLP1Scer\UAS.cUa;SNF4AγMI09417-FF.PT-EGFP/SNF4AγMI09417-TG4.1 flies (employing the Scer\GAL4SNF4Aγ-MI09417-TG4.1 driver inherent in the SNF4AγMI09417-TG4.1 allele to drive the flippase and induce the 'flip-flop' inversion) die as poorly developed pupae and are slim and elongated in the larval stage relative to controls.

      (Nagarkar-Jaiswal et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      SNF4AγMI09417-FF.PT-EGFP
      SNF4AγMI09417EGFP-T2A-mCherry
      (Nagarkar-Jaiswal et al., 2017)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)