FB2024_03 , released June 25, 2024
Allele: Dmel\CirlKO.attP.w-
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General Information
Symbol
Dmel\CirlKO.attP.w-
Species
D. melanogaster
Name
FlyBase ID
FBal0336599
Feature type
allele
Associated gene
Dmel\Cirl
Associated Insertion(s)
TI{TI}CirlKO.attP.w-
Carried in Construct
Also Known As
dCirlKO
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Scholz et al., 2015)
Mutagen
ends-out gene targeting
(Scholz et al., 2015)
cre recombinase
(Scholz et al., 2015)
Progenitor genotype
CirlKO.attP.w+
(Scholz et al., 2015)
Associated Insertion(s)
TI{TI}CirlKO.attP.w-
Cytology
Description

The endogenous Cirl locus (ORF, 5' intergenic region encoding the putative promoter, 5' and 3' UTRs) is replaced with an attP site; the w+* marker has been removed from CirlKO.attP.w+, leaving a single loxP site.

(Scholz et al., 2015)
Allele components
Component
Use(s)
Also carries
attP
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
2R:8,614,551..8,625,291 [+]
Comment:

Position of deletion endpoints on reference sequence determined from reported primer sequences.

(Scholz et al., 2015)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      CirlKO.attP.w- maternal, zygotic mutant embryos showed delayed extension of the embryonic axis and reduced cell intercalation. Wing disc clone boundaries are smother than in control clones.

      (Lavalou et al., 2021)

      CirlKO.attP.w- mutant larvae have no structural or ultrastructural abnormalities in the larval pentascolopidial chordotonal organs (lch5) and do not have any differences in the action current frequencies induced in the lch5 neurons due to optogenetic activation (using Zzzz\ChR2.XXM.UAS.tdTomato expressed under the control of Scer\GAL4iav.PP and activated with 460-500 nm light), compared to controls; the responses of CirlKO.attP.w- mutant lch5 neurons in larvae to mechanical stimulation are significantly reduced, compared to controls, with no effect on their responses to temperature stimuli.

      (Scholz et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      CirlKO.attP.w-
      CirlKO
      (Scholz et al., 2023)
      CirlKO[w-]
      (Lavalou et al., 2021)
      dCirlKO
      (Dannhäuser et al., 2020, Scholz et al., 2017, Scholz et al., 2015)
      dCirlKO w-
      (Scholz et al., 2015)
      Name Synonyms
      Secondary FlyBase IDs
        References (5)