FB2024_03 , released June 25, 2024
Allele: Hsap\RELAAD.12xCRE
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General Information
Symbol
Hsap\RELAAD.12xCRE
Species
H. sapiens
Name
FlyBase ID
FBal0343240
Feature type
allele
Associated gene
Hsap\RELA
Associated Insertion(s)
Carried in Construct
P{12xCRE-p65.AD}
Also Known As
CRE-p65-AD
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Yamazaki et al., 2018)
Progenitor genotype
Carried in construct
P{12xCRE-p65.AD}
Cytology
Description

p65(AD)::Zip+ is under the control of 12 cAMP response element (CRE, TGACGTCA) repeats. A yeast-derived degron (CL1) is attached, to accelerate protein turnover and avoid toxicity.

(Yamazaki et al., 2018)
Allele components
Component
Use(s)
Encoded product / tool
p65(AD)::Zip+
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      alpha'/beta' Kenyon cell | subset

      Comment: when combined with P{Mef2-GAL4.DBD.247}

      (Yamazaki et al., 2018)
      alpha/beta Kenyon cell | subset

      Comment: when combined with P{Mef2-GAL4.DBD.247}

      (Yamazaki et al., 2018)
      gamma Kenyon cell | subset

      Comment: when combined with P{Mef2-GAL4.DBD.247}

      (Yamazaki et al., 2018)
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Inhibition (using shits1.UAS.Tag:MYC at 31[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.Mef2.247 and Hsap\RELAAD.12xCRE in adults during training, consolidation or retrieval significantly reduces 2 h aversive, but not appetitive, olfactory memory, compared to controls.

      Inhibition (using shits1.IVS.p10.20xUAS, but not shits1.UAS.Tag:MYC, at 31[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.Mef2.247 and Hsap\RELAAD.12xCRE in adults, in combination with a paired odor, results in a significant increase in short-term associative attractive olfactory memory in starved, but not fed, flies, compared to controls.

      Inhibition of the combined set of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.Mef2.247 and Hsap\RELAAD.12xCRE and neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R19F09 and Hsap\RELAAD.R25D01 in adults during training, consolidation or retrieval, significantly reduces 2 h appetitive, but not aversive, olfactory memory, compared to controls.

      Thermogenetic activation (using TrpA1UAS.(B).cKa at 31[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.Mef2.247 and Hsap\RELAAD.12xCRE in adults during training, consolidation or retrieval significantly reduces 2 h appetitive, but not aversive, olfactory memory, compared to controls; thermogenetic activation in combination with a paired odor results in a significant increase in short-term associative aversive olfactory memory in fed, but not starved, flies, compared to controls.

      (Yamazaki et al., 2018)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Hsap\RELAAD.12xCRE
      Name Synonyms
      Secondary FlyBase IDs
        References (3)