FB2024_03 , released June 25, 2024
Allele: Dmel\Ndg2
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General Information
Symbol
Dmel\Ndg2
Species
D. melanogaster
Name
FlyBase ID
FBal0344006
Feature type
allele
Associated gene
Dmel\Ndg
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Dai et al., 2018)
Mutagen
CRISPR/Cas9
(Dai et al., 2018)
Progenitor genotype
Cytology
Description

Deletion that removes part of the Ndg transcription unit, including the transcription start site. The CG34222 gene that is located in an intron of Ndg is not affected.

(Dai et al., 2018)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
2R:10,314,244..10,319,546 [+]
Comment:

A 5303bp deletion that removes much of the coding region of Ndg. CG34222 located in a downstream intron of Ndg is not affected.

(Pastor J., 2019.2.13)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Ndg2 mutant adults do not show any obvious morphological abnormalities; homozygous females lay significantly fewer egg but these do not show any obvious morphological defects. Ndg2 homozygotes and Ndg2/Df(2R)BSC281 transheterozygotes show a significantly longer chill coma recovery time, but no defects in flight or climbing activity under control conditions, as compared to controls.

      Ndg1/Ndg2 transheterozygotes show discontinuity of the basement membrane in the larval fat body. Ndg2 homozygotes exhibit significantly smaller lipid droplets in the larval fat body.

      (Dai et al., 2018)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Ndg2
      (Matsubayashi et al., 2020, Pastor J., 2019.2.13, Dai et al., 2018)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)