FB2024_03 , released June 25, 2024
Allele: Scer\GAL4dsx-GAL4(DBD)::Zip-
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General Information
Symbol
Scer\GAL4dsx-GAL4(DBD)::Zip-
Species
S. cerevisiae
Name
FlyBase ID
FBal0344075
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
TI{GAL4(DBD)::Zip-}dsxGAL4(DBD)::Zip-
Carried in Construct
Also Known As
dsx-ZpGal4DBD
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Shirangi et al., 2016)
gene targeting by homologous recombination
(Shirangi et al., 2016)
Progenitor genotype
Associated Insertion(s)
TI{GAL4(DBD)::Zip-}dsxGAL4(DBD)::Zip-
Cytology
Description

A GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain) has been inserted at the start codon within exon 2 of dsx. This results in the hemidriver being expressed under the control of endogenous dsx sequences, along with deletion of exon 2.

(Shirangi et al., 2016)
Allele components
Component
Use(s)
Inserted element
TI{GAL4(DBD)::Zip-}
Encoded product / tool
GAL4(DBD)::Zip-
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      adult doublesex neuron

      Comment: when combined with P{R57C10-p65.AD}

      (Shirangi et al., 2016)
      adult doublesex TN1A (male) neuron

      Comment: when combined with P{VT017258-p65.AD} (combination referred to as 'VT017258- p65AD::Zp ∩ dsx[ Zp::Gdbd ]')

      (Shirangi et al., 2016)
      adult doublesex msB TN2 (male) neuron

      Comment: when combined with P{VT017258-p65.AD} (combination referred to as 'VT017258- p65AD::Zp ∩ dsx[ Zp::Gdbd ]')

      (Shirangi et al., 2016)
      adult doublesex pC1 (female) neuron

      Comment: when combined with P{VT002064-p65.AD}

      when combined with P{VT002064-p65.AD} (combination referred to as 'pC1-SS2')

      when combined with P{VT002064-p65.AD} (combination referred to as 'SS59911')

      (Lillvis et al., 2022, Wang et al., 2021, Wang et al., 2020)
      adult stage | female
      adult doublesex pC1a (female) neuron

      Comment: when combined with P{R18A05-p65.AD} (combination referred to as 'SS75230')

      (Schretter et al., 2020)
      adult doublesex pC1b (female) neuron

      Comment: when combined with P{R18A05-p65.AD} (combination referred to as 'SS75230')

      (Schretter et al., 2020)
      adult doublesex pC1c (female) neuron

      Comment: when combined with P{R18A05-p65.AD} (combination referred to as 'SS75230')

      (Schretter et al., 2020)
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Inhibition (using Ctet\TeTxLCFRT.stop.UAS) of neurons targeted by the split-GAL4 pair of Scer\GAL4dsx-GAL4(DBD)::Zip- and Hsap\RELAAD.VT017258 expressing Scer\FLP1L.8xlexAop under the control of Ecol\lexAGMR24B02 in males results in much less sine song during courtship than controls, but a similar amount of pulse song, with similar inter-pulse interval, carrier frequency and amplitude to controls.

      Acute optogenetic activation (using Cnoc\ChR1Cs.20xUAS.FRT.Venus with 660 nm (red) light activation) of neurons targeted by the split-GAL4 pair of Scer\GAL4dsx-GAL4(DBD)::Zip- and Hsap\RELAAD.VT017258 expressing Scer\FLP1L.8xlexAop under the control of Ecol\lexAGMR24B02 in males, in the absence of females, does not significantly induce courtship song.

      (Shirangi et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Scer\GAL4dsx-GAL4(DBD)::Zip-
      dsxZp::Gdbd
      (Shirangi et al., 2016)
      dsxZpGdbd
      (Shirangi et al., 2016)
      Name Synonyms
      Secondary FlyBase IDs
        References (11)