FB2024_03 , released June 25, 2024
Allele: Dmel\biUAS.cPa
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General Information
Symbol
Dmel\biUAS.cPa
Species
D. melanogaster
Name
FlyBase ID
FBal0344791
Feature type
allele
Associated gene
Dmel\bi
Associated Insertion(s)
Carried in Construct
P{UAS-bi.P}
Also Known As
UAS-omb
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Porsch et al., 2005, Hazelett et al., 1998, Kopp and Duncan, 1997)
Progenitor genotype
Carried in construct
P{UAS-bi.P}
Cytology
Description

UASt sequences drive expression of bi.

(Porsch et al., 2005)

bi is expressed under the control of UAS regulatory sequences.

(Kopp and Duncan, 1997)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
UASt
Encoded product / tool
bi
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  cancer
      CEA
      (Shen et al., 2014)
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      DOES NOT ameliorate  cancer
      modeled by shgUAS.cSa
      (Shen et al., 2014)
      model of  cancer
      is NOT ameliorated by shgUAS.cSa
      (Shen et al., 2014)
      is NOT ameliorated by Diap1UAS.cUa
      (Shen et al., 2014)
      is NOT ameliorated by bskK53R.UAS
      (Shen et al., 2014)
      is NOT ameliorated by TimpUAS.cPa
      (Shen et al., 2014)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      arista, with Scer\GAL4dpp.blk1

      (Porsch et al., 2005)

      epithelial cell | somatic clone | somatic clone | third instar larval stage, with Scer\GAL4Act5C.PI

      (Shen et al., 2014)

      epithelial cell | third instar larval stage, with Scer\GAL430A

      (Shen et al., 2014)

      epithelial cell | third instar larval stage, with Scer\GAL4C-765

      (Shen et al., 2014)

      epithelial cell | third instar larval stage, with Scer\GAL4dpp.PU

      (Shen et al., 2014)

      extracellular matrix | somatic clone | somatic clone | third instar larval stage, with Scer\GAL4Act5C.PI

      (Shen et al., 2014)

      extracellular matrix | third instar larval stage, with Scer\GAL4C-765

      (Shen et al., 2014)

      extracellular matrix | third instar larval stage, with Scer\GAL4dpp.PU

      (Shen et al., 2014)

      eye, with Scer\GAL4dpp.blk1

      (Porsch et al., 2005)

      eye, with Scer\GAL4ey.PH

      (Liu et al., 2018)

      eye, with Scer\GAL4GMR.PF

      (Porsch et al., 2005)

      eye | absent, with Scer\GAL4dpp.blk1

      (Tsai et al., 2015)

      eye disc | cell autonomous | somatic clone | third instar larval stage, with Scer\GAL4Act5C.PU

      (Tsai et al., 2015)

      eye disc | third instar larval stage, with Scer\GAL4dpp.blk1

      (Tsai et al., 2015)

      eye photoreceptor cell, with Scer\GAL4ninaE.PT

      (Liu et al., 2018)

      hypandrial bristle, with Scer\GAL4dpp.blk1

      (Gorfinkiel et al., 1999)

      leg, with Scer\GAL4dpp.blk1

      (Maves and Schubiger, 1998)

      mesothoracic tergum primordium | third instar larval stage, with Scer\GAL4dpp.PU

      (Shen et al., 2014)

      ommatidium | adult stage | decreased number, with Scer\GAL430A

      (Tsai et al., 2015)

      surstylar long bristle | dorsal, with Scer\GAL4dpp.blk1

      (Gorfinkiel et al., 1999)

      tarsal segment, with Scer\GAL4dpp.blk1

      (Porsch et al., 2005)

      tergite | anterior, with Scer\GAL4hs.PB

      (Kopp and Duncan, 1997)

      wing, with Scer\GAL4Bx-MS1096

      (Cook et al., 2004)

      wing, with Scer\GAL4dpp.blk1

      (Grimm and Pflugfelder, 1996)

      wing disc | somatic clone | somatic clone | third instar larval stage, with Scer\GAL4Act5C.PI

      (Shen et al., 2014)

      wing disc | third instar larval stage, with Scer\GAL430A

      (Shen et al., 2014)

      wing disc | third instar larval stage, with Scer\GAL4C-765

      (Shen et al., 2014)

      wing disc | third instar larval stage, with Scer\GAL4dpp.PU

      (Shen et al., 2014)

      wing vein, with Scer\GAL4Bx-MS1096

      (Cook et al., 2004)

      wing vein | ectopic, with Scer\GAL4vg.int2.1

      (Cook et al., 2004)

      wing vein L5, with Scer\GAL4vg.int2.1

      (Cook et al., 2004)
      Detailed Description
      Statement
      Reference

      Expressing biUAS.cPa under the control of Scer\GAL4ey.PH results in small eyes in in a great proportion of adults. Expression under the control of Scer\GAL4ninaE.PT causes a severe loss of synaptic transmission, together with phototransduction defects and abnormal prolonged depolarizing afterpotential (PDA) in eye photoreceptor cells.

      (Liu et al., 2018)

      The expression of biScer\UAS.cBa driven by Scer\GAL430A or Scer\GAL4dpp.PU, or driven in clones by Scer\GAL4Act5C.PI, induces long distance cell migration and scattering in third instar larval wing discs, as compared to controls; clonal expression under the control of Scer\GAL4Act5C.PI also leads to some cells becoming embedded in the extracellular matrix and to some cells elongating along the anterior-posterior axis and exhibiting long actin-rich protrusions; expression under the control of Scer\GAL4dpp.PU also leads to some cells embedding in the extracellular matrix without becoming basally delaminated, but does not lead to any obvious defects regarding cell diameter, cell shape or cell polarity, whereas timed expression (using Gal80[ts]) for 36h prior dissection results in overgrowths in the presumptive notum region of the wing disc, as compared to controls. The expression of biScer\UAS.cBa under the control of Scer\GAL4C-765 results in a proportion of third instar larval wing disc cells becoming embedded in the extracellular matrix and basally delaminating, as cells extrude from the epithelium and accumulate on the other side of the basal membrane, but does not lead to significant cell death, as compared to controls.

      (Shen et al., 2014)

      When biScer\UAS.cPa is driven by Scer\GAL4dpp.blk1, a loss of the arista is seen. Mutant legs exhibit a truncation of tarsal segments. A severe reduction in eye size or the complete loss of retinal tissue is seen. When biScer\UAS.cPa is driven by Scer\GAL4GMR.PF highly degenerated eyes are obtained.

      (Porsch et al., 2005)

      biScer\UAS.cBa; Scer\GAL4Bx-MS1096 flies have small wings in which some or all of the veins are shifted, truncated or completely missing. Wing veins L2 and L5 are particularly stringly affected. biScer\UAS.cBa; Scer\GAL4vg.int2.1 flies have distally truncated L5 wing veins and occasionally have ectopic veins posterior to L5.

      (Cook et al., 2004)

      When biScer\UAS.cBa is overexpressed under the control of Scer\GAL4dpp.blk1, the dorsal bristles of the clasper and the hypandrium bristles are duplicated.

      (Gorfinkiel et al., 1999)

      Scer\GAL4dpp.blk1-mediated expression dorsalises ventral leg cells (loss of ventral leg structures, fusion of proximal foreleg segments and ventral leg bifurcations) but is not sufficient to induce transdetermination. Legs lack ventral leg structures and have a mirror-image dorsal-dorsal symmetry.

      (Maves and Schubiger, 1998)

      Flies in which biScer\UAS.cBa is expressed under the control of Scer\GAL4hs.PB using a single 2-hour heat shock between 12 and 30 hours after puparium formation die prior to tergite pigmentation. The anterior tergite and intersegmental membrane of these pharate adults are completely replaced by a mirror-image duplication of posterior tergite and posterior hairy zone. No ectopic macrochaetae are seen.

      (Kopp and Duncan, 1997)

      When expressed from Scer\GAL4dpp.blk1, wing outgrowths of variable expressivity are observed in 80% of flies. Ectopic wings are located postero-dorsally and mirror-symmetrically to the normal wing, as indicated by the opposing alulae.

      (Grimm and Pflugfelder, 1996)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      NOT suppressed by
      Statement
      Reference

      Scer\GAL4dpp.blk1, biUAS.cPa has eye phenotype, non-suppressible by BacA\p35UAS.cUa, Scer\GAL4dpp.blk1

      (Tsai et al., 2015)

      Scer\GAL4C-765, biUAS.cPa has extracellular matrix | third instar larval stage phenotype, non-suppressible by Scer\GAL4C-765/bskK53R.UAS

      (Shen et al., 2014)

      Scer\GAL4C-765, biUAS.cPa has wing disc | third instar larval stage phenotype, non-suppressible by Diap1UAS.cUa, Scer\GAL4C-765

      (Shen et al., 2014)

      Scer\GAL4C-765, biUAS.cPa has epithelial cell | third instar larval stage phenotype, non-suppressible by Diap1UAS.cUa, Scer\GAL4C-765

      (Shen et al., 2014)

      Scer\GAL4C-765, biUAS.cPa has extracellular matrix | third instar larval stage phenotype, non-suppressible by Diap1UAS.cUa, Scer\GAL4C-765

      (Shen et al., 2014)

      Scer\GAL4dpp.PU, biUAS.cPa has wing disc | third instar larval stage phenotype, non-suppressible by shgUAS.cSa/Scer\GAL4dpp.PU

      (Shen et al., 2014)

      Scer\GAL4dpp.PU, biUAS.cPa has epithelial cell | third instar larval stage phenotype, non-suppressible by shgUAS.cSa/Scer\GAL4dpp.PU

      (Shen et al., 2014)

      Scer\GAL4C-765, biUAS.cPa has wing disc | third instar larval stage phenotype, non-suppressible by Scer\GAL4C-765/TimpUAS.cPa

      (Shen et al., 2014)

      Scer\GAL4C-765, biUAS.cPa has epithelial cell | third instar larval stage phenotype, non-suppressible by Scer\GAL4C-765/TimpUAS.cPa

      (Shen et al., 2014)

      Scer\GAL4C-765, biUAS.cPa has extracellular matrix | third instar larval stage phenotype, non-suppressible by Scer\GAL4C-765/TimpUAS.cPa

      (Shen et al., 2014)

      Scer\GAL4dpp.PU, biUAS.cPa has wing disc | third instar larval stage phenotype, non-suppressible by Scer\GAL4dpp.PU/TimpUAS.cPa

      (Shen et al., 2014)

      Scer\GAL4dpp.PU, biUAS.cPa has epithelial cell | third instar larval stage phenotype, non-suppressible by Scer\GAL4dpp.PU/TimpUAS.cPa

      (Shen et al., 2014)

      Scer\GAL4dpp.PU, biUAS.cPa has wing disc | third instar larval stage phenotype, non-suppressible by Scer\GAL4dpp.PU/bskK53R.UAS

      (Shen et al., 2014)

      Scer\GAL4Act5C.PI, biUAS.cPa has wing disc | somatic clone | third instar larval stage phenotype, non-suppressible by Scer\GAL4Act5C.PI/bskK53R.UAS

      (Shen et al., 2014)

      Scer\GAL4C-765, biUAS.cPa has wing disc | third instar larval stage phenotype, non-suppressible by Scer\GAL4C-765/bskK53R.UAS

      (Shen et al., 2014)

      Scer\GAL4dpp.PU, biUAS.cPa has epithelial cell | third instar larval stage phenotype, non-suppressible by Scer\GAL4dpp.PU/bskK53R.UAS

      (Shen et al., 2014)

      Scer\GAL4Act5C.PI, biUAS.cPa has epithelial cell | somatic clone | third instar larval stage phenotype, non-suppressible by Scer\GAL4Act5C.PI/bskK53R.UAS

      (Shen et al., 2014)

      Scer\GAL4C-765, biUAS.cPa has epithelial cell | third instar larval stage phenotype, non-suppressible by Scer\GAL4C-765/bskK53R.UAS

      (Shen et al., 2014)
      NOT Enhancer of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The long distance cell migration and scattering, resulting from the expression in third instar larval wing discs of biScer\UAS.cBa either under the control of Scer\GAL4dpp.PU, or in clones under the control of Scer\GAL4Act5C.PI, is not suppressed by the co-expression of bskK53R.Scer\UAS; the basal delamination of cells, resulting from the expression of biScer\UAS.cBa under the control of Scer\GAL4C-765 in third instar larval wing discs, is not suppressed by the co-expression of bskK53R.Scer\UAS, Diap1Scer\UAS.cUa or TimpScer\UAS.cPa.

      The co-expression of both biScer\UAS.cBa and shgScer\UAS.cSa under the control of Scer\GAL4dpp.PU does not significantly suppress the long distance cell migration and scattering observed in third instar larval wing discs upon the expression of only biScer\UAS.cBa or only shgScer\UAS.cSa, under the control of Scer\GAL4dpp.PU.

      (Shen et al., 2014)

      wgAct5C.PS; Scer\GAL4dpp.blk1/biScer\UAS.cBa pupae die before differentiating adult cuticle, transplanted leg discs exhibit an extensive area of wing tissue.

      (Maves and Schubiger, 1998)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      biUAS.cPa fails to rescue biMI08152-TG4.0

      (Liu et al., 2018)
      Comments

      Expressing biUAS.cPa under the control of Scer\GAL4bi-MI08152-TG4.0 fails to rescue pupal lethality in biMI08152-TG4.0 hemizygous males.

      (Liu et al., 2018)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer

      Balles, Grimm, Hofmeyer and Pflugfelder.

      (Grimm and Pflugfelder, 1996)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      biScer\UAS.cBa
      biScer\UAS.cPa
      biUAS.cBa
      biUAS.cPa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Porsch
      (Porsch et al., 2005)
      Secondary FlyBase IDs
      • FBal0180796
      • FBal0049358
      References (15)