FB2024_03 , released June 25, 2024
Allele: Hsap\RELAAD.R48H08
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General Information
Symbol
Hsap\RELAAD.R48H08
Species
H. sapiens
Name
FlyBase ID
FBal0345553
Feature type
allele
Associated gene
Hsap\RELA
Associated Insertion(s)
Carried in Construct
P{R48H08-p65.AD}
Also Known As
R48H08_p65ADZp
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(von Reyn et al., 2017)
Progenitor genotype
Carried in construct
P{R48H08-p65.AD}
Cytology
Description

The GMR48H08 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).

(von Reyn et al., 2017)
Allele components
Component
Use(s)
Regulatory region(s)
GMR48H08
Encoded product / tool
p65(AD)::Zip+
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      lamina monopolar neuron L1

      Comment: when combined with P{R29G11-GAL4.DBD}

      (von Reyn et al., 2017)
      lamina monopolar neuron L2

      Comment: when combined with P{R29G11-GAL4.DBD}

      (von Reyn et al., 2017)
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Optogenetic activation (using Cnoc\ChR1Cs.IVS.20xUAS.Venus with 590 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R29G11 and Hsap\RELAAD.R48H08 in adults does not affect takeoff rates, compared to controls; spontaneous takeoffs are similar in duration to those of controls.

      Silencing (using Hsap\KCNJ2IVS.10xUAS.EGFP) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R29G11 and Hsap\RELAAD.R48H08 in adults substantially and significantly reduces takeoff rates in response to looming stimuli, compared to controls; it also eliminates all aspects of giant fiber cell activity seen in controls in response to looming stimuli.

      (von Reyn et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Hsap\RELAAD.R48H08
      Name Synonyms
      Secondary FlyBase IDs
        References (3)