FB2024_03 , released June 25, 2024
Allele: Dmel\dbfz3318
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General Information
Symbol
Dmel\dbfz3318
Species
D. melanogaster
Name
FlyBase ID
FBal0357247
Feature type
allele
Associated gene
Dmel\dbf
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Sechi et al., 2019)
Progenitor genotype
Cytology
Description

Amino acid replacement: R85term.

(Sechi et al., 2019)

Produces a truncated protein containing 84 out of 652 amino acids.

(Sechi et al., 2019)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2L:10,652,367..10,652,367 [+]
Nucleotide change:

C10652367T

Amino acid change:

R85term | dbf-PA

Reported amino acid change:

R85term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change.

(Sechi et al., 2019)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      dbf1/dbfz3318 or dbfz3318/Df(2L)Exel8026 males exhibit aberrant spermatids containing mitochondrial derivatives of variable size associated with nuclei that are also different in size and number.

      In dbfz3318/Df(2L)Exel8026 males, one chromosome pair (specifically the X-Y bivalent chromatin) fails to condense properly; and there are a variable number of centrosomal foci and assembled monopolar or multipolar spindles in dividing spermatocytes.

      Most S5-S6 dbfz3318/Df(2L)Exel8026 primary spermatocytes display more than two Asl-labeled foci.

      dbfz3318/Df(2L)Exel8026 dividing spermatocytes exhibit an increase in the cilium-like projections size at stages M2-M3.

      In dbfz3318/Df(2L)Exel8026 spermatids, axonemes lost integrity and the doublets spread, isolated microtubule doublets or triplets are observed instead of canonical 9+2 axonemes.

      dbfz3318/Df(2L)Exel8026 males exhibit irregular spindles, with either multipartite or bipartite central spindles asymmetrically located with respect to cell poles in most ana-telophases. Moreover all the ana-telophase exhibit irregular chromosome segregation.

      (Sechi et al., 2019)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescued by

      dbfz3318/Df(2L)Exel8026 is partially rescued by dbfαTub.EGFP

      (Sechi et al., 2019)

      dbfz3318/Df(2L)Exel8026 is partially rescued by dbfαTub.mRFP(Unk)

      (Sechi et al., 2019)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      From the Zuker collection of male sterile mutants.

      (Sechi et al., 2019)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      dbfz3318
      (Sechi et al., 2019)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)