The tup ADME enhancer element (coordinates 2L:18899122..18900688 , release 6 genome), in which the 3 org-1 protein binding sites have been mutated (TAACACAT to TAAGCTTT, GGGTGCCA to GGCTCGAG and TGGTGGGA to TGTCTAGA), regulates expression of EGFP tagged with Tag:NLS(tra).