The entire coding region of the endogenous Ms gene has been deleted. A sequence cassette that includes 1. an attP site and 2. an excisable cassette that contains a Disc\RFPmCherry.3xP3 marker has been inserted in place of the deleted sequence.
The entire Ms coding sequence has been deleted and replaced with a cassette that includes and attP site and an excisable cassette containing a Disc\RFPmCherry.3xP3 marker.