FB2024_03 , released June 25, 2024
Allele: Dmel\Lkb1ΔLB.C546A.GFP
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General Information
Symbol
Dmel\Lkb1ΔLB.C546A.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0367743
Feature type
allele
Associated gene
Dmel\Lkb1
Associated Insertion(s)
Carried in Construct
P{GFP-Lkb1.ΔLB.C546A}
Key Links
Transgenic product class
missense_variant
(Dogliotti et al., 2017)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Dogliotti et al., 2017)
Mutagen
in vitro construct
(Dogliotti et al., 2017)
Progenitor genotype
Carried in construct
P{GFP-Lkb1.ΔLB.C546A}
Cytology
Description

Genomic fragment that encompasses Lkb1; extends from 2.8 kb upstream of the translation start site to 1kb downstream of the stop codon. The C-terminal lipid-binding (LB) motif has been mutated (amino acid substitutions K546A, R547A, R548A, K550A, K551A, K539A, K540A, K541A); this abolishes the ability of the protein to bind liposomes in vitro. In addition, a C564A amino acid substitution (expected to result in a protein that cannot be farnesylated) has also been introduced into the coding sequence. A GFP tag has been inserted at the N-terminal end of the coding sequence.

(Dogliotti et al., 2017)
Allele components
Component
Use(s)
Regulatory region(s)
Lkb1
Encoded product / tool
Lkb1
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Lkb1ΔLB.C546A.GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)