Sequence between the first intron and 3'UTR of the ci locus has been replaced with mutated genomic ci sequence, removing the w+* marker present in TI{TI}ciw+ and reconstituting a ci locus that carries a S849A amino acid substitution; this results in a protein that lacks the last CK1 target site initially primed by PKA phosphorylation of S838. The inserted sequence also contains a Tag:FLAG and a Tag:HA tag, upstream and downstream of the ci coding sequence respectively.