FB2024_03 , released June 25, 2024
Allele: Equa\eqFP578tdKatushka2.nanos.cKa.Tag:CAAX(hKRAS)
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General Information
Symbol
Equa\eqFP578tdKatushka2.nanos.cKa.Tag:CAAX(hKRAS)
Species
E. quadricolor
Name
FlyBase ID
FBal0384551
Feature type
allele
Associated gene
Equa\eqFP578
Associated Insertion(s)
Carried in Construct
P{nanos-scraRBD::tdT-2A-tdKatushka2::CAAX}
Also Known As
nos-tdTomato-Anillin-RBD-P2A-tdKatushka2-CAAX
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Kong et al., 2019)
Progenitor genotype
Carried in construct
P{nanos-scraRBD::tdT-2A-tdKatushka2::CAAX}
Cytology
Description

nanos regulatory elements (promoter, 5'UTR and 3'UTR) regulate expression of a transcript that encodes two open reading frames separated by a 2A linker (this results in separate translation of each protein). The first ORF encodes the scra Rho-binding domain (RBD) (amino acids 748-1239) tagged with tdTomato; this protein acts as a sensor of Rho activity. The second ORF encodes tdKatushka2 tagged with the Tag:CAAX(hKRAS) membrane targeting sequence.

(Kong et al., 2019)
Allele components
Component
Use(s)
Regulatory region(s)
nanos
Encoded product / tool
tdKatushka2
Tagged with
Tag:CAAX(hKRAS)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Equa\eqFP578tdKatushka2.nanos.cKa.Tag:CAAX(hKRAS)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)