FlyBase Allele Report: Dmel\GluRIIC[RNAi.UAS.cBa]

General Information

Symbol

Dmel\GluRIIC^RNAi.UAS.cBa

Species

D. melanogaster

Name

FlyBase ID

FBal0391979

Feature type

allele

Associated gene

Dmel\GluRIIC

Associated Insertion(s)

Carried in Construct

P{UAS-GluRIIC.RNAi.B}

Also Known As

UAS-GluRIII[RNAi]

Key Links

Transgenic product class

RNAi_reagent

(Brusich et al., 2015)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Brusich et al., 2015)

Mutagen

in vitro construct

(Brusich et al., 2015)

Progenitor genotype

Carried in construct

P{UAS-GluRIIC.RNAi.B}

Cytology

Description

UAS regulatory sequences drive expression of sequences targeting GluRIIC.

(Brusich et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

GluRIIC

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology, with Scer\GAL4^C57

(Brusich et al., 2015)

abnormal neurophysiology, with Scer\GAL4^C57, Scer\GAL4^elav-C155, Scer\GAL4^sca.PC

(Brusich et al., 2015)

abnormal neurophysiology | third instar larval stage, with Scer\GAL4^C57, Scer\GAL4^elav-C155, Scer\GAL4^sca.PC

(Spring et al., 2016)

Phenotype Manifest In

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^C57, Scer\GAL4^elav-C155, Scer\GAL4^sca.PC

(Spring et al., 2016)

Detailed Description

Statement

Reference

Expression of GluRIIC^{dsRNA.Scer\UAS.cBa} driven simultaneously by Scer\GAL4^elav-C155, Scer\GAL4^sca.PC and Scer\GAL4^C57 significantly diminishes miniature excitatory postsynaptic potential (mEPSP) amplitudes (decreasing quantal size), leading to significant homeostatic compensatory increases in quantal content at the neuromuscular junction (NMJ) of third instar larvae, compared to controls.

(Spring et al., 2016)

Larvae expressing GluRIIC^{dsRNA.Scer\UAS.cBa} under the control of Scer\GAL4^C57 show a decrease in spontaneous miniature neurotransmission. A compensatory increase in quantal content means that evoked neurotransmission is unaffected.

When female flies expressing GluRIIC^{dsRNA.Scer\UAS.cBa} under the control of multiple pre- and post-synaptic drivers (Scer\GAL4^elav-C155, Scer\GAL4^sca.PC and Scer\GAL4^C57) (aka T15 flies) are crossed to wild type males the resulting larvae exhibit defects in spontaneous neurotransmission. A compensatory increase in quantal content means that evoked neurotransmission is unaffected. When T15 males are crossed to wild type females neurotransmission is similar to controls. Synapse growth and morphology are grossly normal.

(Brusich et al., 2015)

Expression of GluRIIC^{dsRNA.Scer\UAS} in muscle throughout development (under the control of Scer\GAL4^C57) causes a reduction in mEJP amplitude and a homeostatic increase in presynaptic release.

(Dickman et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scer\GAL4^sca.PC, GluRIIC^RNAi.UAS.cBa, Scer\GAL4^elav-C155, Scer\GAL4^C57 is an enhancer of abnormal neurophysiology phenotype of Scer\GAL4^C57, Scer\GAL4^elav-C155, Scer\GAL4^sca.PC, cac^KK101478

(Brusich et al., 2015)

Other

Statement

Reference

Phenotype Manifest In

Other

Statement

Reference

Fas2^EP1462, GluRIIC^RNAi.UAS.cBa, Scer\GAL4^C57, Scer\GAL4^elav-C155, Scer\GAL4^sca.PC has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Spring et al., 2016)

Csk^GD9345, GluRIIC^RNAi.UAS.cBa, Scer\GAL4^C57, Scer\GAL4^elav-C155, Scer\GAL4^sca.PC has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Spring et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Third instar larvae with co-expression of GluRIIC^{dsRNA.Scer\UAS.cBa} and Csk^GD9345 driven simultaneously by Scer\GAL4^elav-C155, Scer\GAL4^sca.PC and Scer\GAL4^C57 show significant decreases in mEPSP (decreasing quantal size) but do not show significant compensatory homeostatic increases in quantal content (as seen with expression of GluRIIC^{dsRNA.Scer\UAS.cBa} alone) at the NMJ; larvae with co-expression show diminished evoked muscle excitation compared to larvae with expression of GluRIIC^{dsRNA.Scer\UAS.cBa} alone.

Third instar larvae with co-expression of GluRIIC^{dsRNA.Scer\UAS.cBa} and Csk^{Scer\UAS.T:Avic\GFP-YFP} driven simultaneously by Scer\GAL4^elav-C155, Scer\GAL4^sca.PC and Scer\GAL4^C57 show significant decreases in mEPSP (decreasing quantal size) and show significant compensatory homeostatic increases in quantal content (as seen with expression of GluRIIC^{dsRNA.Scer\UAS.cBa} alone) at the NMJ.

Third instar larvae with co-expression of GluRIIC^{dsRNA.Scer\UAS.cBa} and Fas2^EP1462 driven simultaneously by Scer\GAL4^elav-C155, Scer\GAL4^sca.PC and Scer\GAL4^C57 show significant decreases in mEPSP (decreasing quantal size) and show significant (though not complete) impairment of compensatory homeostatic increases in quantal content (compared to quantal content increases seen with expression of GluRIIC^{dsRNA.Scer\UAS.cBa} alone) at the NMJ.

Co-expression of Fas2^Scer\UAS.cLa, Fas2^Scer\UAS.cLb or Fas2^C.Scer\UAS in third instar larvae with GluRIIC^{dsRNA.Scer\UAS.cBa} simultaneously driven by Scer\GAL4^C57 and Scer\GAL4^elav-C155 does not impair homeostatic compensation at the neuromuscular junction (significant reduction in mEPSP and significant increase in quantal content compared to controls, just as is seen in larvae with expression of GluRIIC^{dsRNA.Scer\UAS.cBa} simultaneously driven by Scer\GAL4^C57 and Scer\GAL4^elav-C155).

Fas2^e76/Y or Fas2^e76/Fas2^G0293 third instar larvae with GluRIIC^{dsRNA.Scer\UAS.cBa} driven by Scer\GAL4^C57 do not show impairment of homeostatic compensation at the neuromuscular junction (significant reduction in mEPSP and significant increase in quantal content compared to controls, just as is seen in larvae with expression of GluRIIC^{dsRNA.Scer\UAS.cBa} driven by Scer\GAL4^C57).

Co-expression of Fas2^JF02918 or Fas2^GD14486 in third instar larvae with GluRIIC^{dsRNA.Scer\UAS.cBa} simultaneously driven by Scer\GAL4^C57, Scer\GAL4^elav-C155 and Scer\GAL4^sca.PC does not impair homeostatic compensation at the neuromuscular junction (significant reduction in mEPSP and significant increase in quantal content compared to controls, just as is seen in larvae with expression of GluRIIC^{dsRNA.Scer\UAS.cBa} simultaneously driven by Scer\GAL4^C57, Scer\GAL4^elav-C155 and Scer\GAL4^sca.PC).

(Spring et al., 2016)

Expression of GluRIIC^{dsRNA.Scer\UAS.cBa} enhances the reduction in quantal size seen in the larval NMJ when cac^KK101478 is expressed in multiple pre- and post-synaptic drivers (Scer\GAL4^elav-C155, Scer\GAL4^sca.PC and Scer\GAL4^C57) alone. However, the defects in evoked transmission are not enhanced due to a compensatory increase in presynaptic quantal content.

Expression of Csp^GD10571 completely prevents the compensatory increase in quantal content seen in response to reduced quantal size when GluRIIC^{dsRNA.Scer\UAS.cBa} is expressed using multiple pre- and post-synaptic drivers (Scer\GAL4^elav-C155, Scer\GAL4^sca.PC and Scer\GAL4^C57). Evoked neurotransmission is therefore disrupted.

Expression of Csp^GD10571 does not prevent the compensatory increase in quantal content seen in response to reduced quantal size when GluRIIC^{dsRNA.Scer\UAS.cBa} is expressed under the control of Scer\GAL4^C57. Evoked neurotransmission is unaffected.

Expression of Plc21C^GD11359 prevents the compensatory increase in quantal content seen in response to reduced quantal size when GluRIIC^{dsRNA.Scer\UAS.cBa} is expressed using multiple pre- and post-synaptic drivers (Scer\GAL4^elav-C155, Scer\GAL4^sca.PC and Scer\GAL4^C57). Evoked neurotransmission is therefore disrupted. Muscle expression of Plc21C^GD11359 (under the control of Scer\GAL4^C57) do not prevent the compensatory increase and evoked neurotransmission is unaffected.

Expression of Plc21C^HMS00600 prevents the compensatory increase in quantal content seen in response to reduced quantal size when GluRIIC^{dsRNA.Scer\UAS.cBa} is expressed using multiple pre- and post-synaptic drivers (Scer\GAL4^elav-C155, Scer\GAL4^sca.PC and Scer\GAL4^C57).

Expression of Plc21C^JF01211 prevents the compensatory increase in quantal content seen in response to reduced quantal size when GluRIIC^{dsRNA.Scer\UAS.cBa} is expressed using multiple pre- and post-synaptic drivers (Scer\GAL4^elav-C155, Scer\GAL4^sca.PC and Scer\GAL4^C57).

Expression of Plc21C^JF01210 prevents the compensatory increase in quantal content seen in response to reduced quantal size when GluRIIC^{dsRNA.Scer\UAS.cBa} is expressed using multiple pre- and post-synaptic drivers (Scer\GAL4^elav-C155, Scer\GAL4^sca.PC and Scer\GAL4^C57).

(Brusich et al., 2015)

The developmental homeostatic plasticity that normally occurs in larvae expressing GluRIIC^{dsRNA.Scer\UAS.cFa} in the muscle under the control of Scer\GAL4^C57 is completely blocked if the larvae are also expressing P{GD12574} in the nervous system (under the simultaneous control of both Scer\GAL4^elav-C155 and Scer\GAL4^sca.PC).

(Dickman et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

GluRIIC^RNAi.UAS.cBa

GluRIIC^{dsRNA.Scer\UAS.cBa}

GluRIIC^{dsRNA.Scer\UAS.cDa}

GluRIIC^{dsRNA.Scer\UAS.cFa}

GluRIIC^{dsRNA.UAS.cBa}

GluRIIC^{dsRNA.UAS.cFa}

Name Synonyms

Saccharomyces cerevisiae UAS construct of Brusich

Secondary FlyBase IDs

FBal0282371
FBal0316437

References (4)

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