FB2024_03 , released June 25, 2024
Clone: Dmel\EC00586
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General Information
Symbol
Dmel\EC00586
Species
D. melanogaster
Name
EC00586
FlyBase ID
FBcl0062493
Feature type
cDNA_clone
Computed gene(s)
Dmel\CG30324
 
Collection Status
N/A
Known Problems
none
FlyBase assessment
    Library
    EC_cDNA
    • Exelexis EC cDNA library of bacterially challenged D. melanogaster, iso-1 strain, larva, fat body.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage
      embryonic/larval fat body
      (Carlson, 2005.9.12)
      Sequence Data of the Insert
      3' Sequence
      Total bases
      421
      NCBI
      CO179943
      3' sequence dataSequence Downloader
      ACTTCTCACATTCCGAACTTTTATTAAACTCTTTTTTCCCCAATTGAGTCCTGCCCAGGATTACAAGTCATAGCAGAGCG
ATTTATTGGTACAGGGACAACCATCGATGCCGTAACGCCCAATCGGAATGTCCGTCTGGGTCTCCTTGTGCTGCGGTCCT
GGCAACTCGTAGAATTTCTTACCAAAGTATCGATCCGGGAGATAGTACGATGGATACTCCGCCCTCAGCCTCTCGTGATC
CTCCCGCAGAAATTTTGCCAGTTCGGGAGTAGCCCGTCGAGCTTCCAGGCGATACTGCTTCGGCTTGGCCATTCGGATTG
GAACTTCCAGGTGGAGCTTCGAGTGCGGTATGTAGGACAGCAGCTCCTGGTCGAGTTTCACTGCCTGCTCTGCACGAATT
TGTGCGCAAGTGGCACACTTG
      Library Information (1)
      Library: EC_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from fat bodies of immunologically challenged third instar larvae. The strain used was not the iso-1 sequenced strain.

      (Carlson, 2005.9.12)
      Sample preparation

      The fat body was dissected from third instar larva challenged with gram positive and gram negative bacteria.

      Protocol

      RNA was isolated from fat body from 3rd instar larva challenged with gram+/- bacteria; cDNA was oligo(dT) primed.

      (Carlson, 2005.9.12)
      Comment

      The Exelixis ESTs were submitted to GenBank by the BDGP on behalf of Exelixis.

      (Carlson, 2005.9.12)
      Reported As
      Symbol Synonyms
      EC
      EC_cDNA
      ML01
      (Chen et al., 2005)
      References (2)
      Personal communication to FlyBase (1)
      Carlson, 2005.9.12, FlyTag EST project.
      FlyTag EST project. [FBrf0188689]
      Unpublished (1)
      Chen et al., 2005, FlyTag ML01 EST Project.
      FlyTag ML01 EST Project. [FBrf0184942]
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)