FB2024_03 , released June 25, 2024
Clone: Dmel\IP10321
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General Information
Symbol
Dmel\IP10321
Species
D. melanogaster
Name
IP10321
FlyBase ID
FBcl0319483
Feature type
cDNA_clone
Computed gene(s)
Dmel\RpL35A
 
Collection Status
N/A
Known Problems
none
FlyBase assessment
    Library
    IP_cDNA
    • IP cDNA library of D. melanogaster, isolated by an inverse-PCR screen (SLIP) from other cDNA libraries.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage
      organism

      Comment: 0-22 hr AEL

      (Rubin et al., 2000)
      larval stage
      organism
      (Rubin et al., 2000)
      pupal stage
      organism

      Comment: early pupae

      (Rubin et al., 2000)
      adult stage
      adult head
      (Rubin et al., 2000)
      Sequence Data of the Insert
      3' Sequence
      Total bases
      588
      NCBI
      EL880476
      3' sequence dataSequence Downloader
      AAATTTAAAGTGTTTCATTTAATGTAAAACGGATTTTTTACTCCTGCAGGTCAGTAATTCAAGTCGGATATTAACTTAAA
TCCTTGATGGGTACAGCATGATGCGGATGCGGTGGCCCATGGCATGACCGGGCAGGTTCCTGTTGAAACGGGCACGCACA
GCGCCGGTGTTGCCGTGGATGCGGGTGACCTTGCCCCAGACAGCGCGGACGCGGGTCTTGCGCTCGGGATGCTGCGGCAC
GCACTTCTTGGTCTCGGCCTTGTAGACATAGACGCAACGCTTCCCAACGTAGAAGGATCCGTGCTCCTTGCGGCGGGCGC
CCTCAATCTTGAGGATGGCCTGGTTCTCGTGCTGGTTCCTCAGACCACGCTTGTAGCCGGTGAAGACGGCCTTGGCGAAG
AGGCGTCCGTGACGCTTGTACTTCTTGGCGGAAACTTTAGCCTCTGAGGCGGCGGGCTTCTCGGCCTTAGGCGCCTTGAC
GGCCTTGGGAGCCTTCTGGGCCTTGGCGGCCTTGGGCGCAGTAGTGGACTTGGCTTGTGTGTCGGCCATGGCTTAGCTTG
TTGTTCGATGAATGTATTAACACCGTTC
      5' Sequence
      Total bases
      594
      NCBI
      EL880477
      5' sequence dataSequence Downloader
      GCGAACGGTGTTAATACATTCATCGAACAACAAGCTAAGCCATGGCCGACACACAAGCCAAGTCCACTACTGCGCCCAAG
GCCGCCAAGGCCCAGAAGGCTCCCAAGGCCGTCAAGGCGCCTAAGGCCGAGAAGCCCGCCGCCTCAGAGGCTAAAGTTTC
CGCCAAGAAGTACAAGCGTCACGGACGCCTCTTCGCCAAGGCCGTCTTCACCGGCTACAAGCGTGGTCTGAGGAACCAGC
ACGAGAACCAGGCCATCCTCAAGATTGAGGGCGCCCGCCGCAAGGAGCACGGATCCTTCTACGTTGGGAAGCGTTGCGTC
TATGTCTACAAGGCCGAGACCAAGAAGTGCGTGCCGCAGCATCCCGAGCGCAAGACCCGCGTCCGCGCTGTCTGGGGCAA
GGTCACCCGCATCCACGGTAACACCGGCGCTGTGCGTGCCCGTTTCAACAGGAACCTGCCCGGTCATGCCATGGGCCACC
GCATCCGCATCATGCTGTACCCATCAAGGATTTAAGTTAATATCCGACTTGAATTACTGACCTGCAGGAGTAAAAAATCC
GTTTTACATTAAATGAAACCCTTTAAACTCAAAA
      Library Information (1)
      Library: IP_cDNA
      Description

      Clones isolated by an inverse-PCR screen (SLIP) using a mixture of the GH, LD, LP, and SD cDNA libraries.

      Sample preparation

      Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      These clones have been isolated a directed screening process called SLIP (Self-Ligation of Inverse PCR Products). Clones isolated from this screen have been PCR-amplified using a mixture of 4 different plasmid cDNA libraries as template. These include the GH, LD, LP, and SD cDNA libraries.

      (FlyBase Curators, 2016-)
      Comment
      Reported As
      Symbol Synonyms
      IP
      IP_cDNA
      References (1)
      FlyBase analysis (1)
      FlyBase Curators, 2016-, Curation of large dataset metadata by FlyBase.
      Curation of large dataset metadata by FlyBase. [FBrf0232540]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)