FB2024_03 , released June 25, 2024
Clone: Dmel\IP15119
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General Information
Symbol
Dmel\IP15119
Species
D. melanogaster
Name
IP15119
FlyBase ID
FBcl0319514
Feature type
cDNA_clone
Computed gene(s)
Dmel\plx, Dmel\CG31705
 
Collection Status
N/A
Known Problems
none
FlyBase assessment
    Library
    IP_cDNA
    • IP cDNA library of D. melanogaster, isolated by an inverse-PCR screen (SLIP) from other cDNA libraries.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage
      organism

      Comment: 0-22 hr AEL

      (Rubin et al., 2000)
      larval stage
      organism
      (Rubin et al., 2000)
      pupal stage
      organism

      Comment: early pupae

      (Rubin et al., 2000)
      adult stage
      adult head
      (Rubin et al., 2000)
      Sequence Data of the Insert
      5' Sequence
      Total bases
      924
      NCBI
      EL875075
      5' sequence dataSequence Downloader
      CCCGTTCTGCCGGCGGGCCTTAATCGCGGTAAAATCCGCGCAGCAGTTTCCCAATAAATCAGAACCGTTAGCTGCGACCG
GTGGTCGCAGTGGCCGCCGGTGGGGCCAGCTCCACGGGCACGGGAGCCACTCGCACCGCGGGCTCCGTGCTGGATGGATG
CTGTACTCCACCCAGCGGGATCGCACGCATGTGGTGCACCGGACGTATGGATTTGAGCTGCGTGGTGCCCTTGAAGCGTA
CGTTAACATCGCCCCCGACCATGCTGAGCGGATGCATGGGCTGCGGCTCCTCGACATCCTCCTGAGGCATTACAGCCATT
GTCGTTGCGGGCGCCACCTCCTCCATGTGCTGCGGGCATGGTGCTGTGGTAATTGCCACGCCATAACTTTGTCGCTGGGC
CAGCGGGGAAAGGGCTGGGGGCGTTGTCCGATAGCCCATGCGGCTGAATATGCTTCCTCCGCTGCTGTTACTCGCTGTGC
TGGGCGGGCTGAGGGGCGTGCCTACGCCAGAATCTACCTCCAAGGGACTTTTGATATTTGCTACGAACTTATCCACGTGC
TCCGGCAGCTTCAGCTCATCCAGTTTCACTTGCATGACGGTCCGAGCGCTGGCATTGTCCAGCAGGCGATTGGGTCGCGA
AGGTGGAGTCGGGGACCCTGACTCACCGACATTAGATGACTGGCCGCCGCCGTTGAGGCGATGCTGTTGCTGCTGCTGAC
GCAACTGCTCAAAAAAAGGTGTCTTCTCCTTCTTCTGCTTTTGTGGCGAACCAAGTTCGTCTCTTTCGGTCAGCTCTTCG
AGCACCTTGAGAGGAAACCCCAAGTGACTGTTGACGGAAACGGATTTGCCGATCTTGCGTTCGGTGAATATGGGCTTCCG
ACGCTGACGGTCCAAGTCGTCCAGCTGCTGGAGCATGCGCCGCA
      3' Sequence
      Total bases
      923
      NCBI
      EL875074
      3' sequence dataSequence Downloader
      ACAAAATACATTTAAGGAAAACCGTTTGACCGGCGATAGACATATATGCATATCCATAAGAATCTTGAGTCCCATAAAAT
GGCAATAAATTACGGTAGCCAACGAGACTGTGTCCTTGAGTCAATTAAGGATTGTATGGAGGCAATGGGCCGTTAGTGGA
GACAGTAGGCCACGATTAGGGTCACCACCGTGGAGATGAAGCTAAAGTGTCCAAAAGTTCGCTCTGCCATACTATGCGCA
TCCTTGACGATAGATTCCGCCGAGAGTCGAGCCTGTGTCAGACCCGGCATCTTCATAATATCGGGCTGACCACCGGGAAC
TATGGGTGCCAATGGTACAGATCCCTCGGGAATGGCCACAGGAGTGGTCGGCTGAATGGGAGCCAGTTGAACGGATTCGG
GAGCGGAAACGGGAGGGGGTACTGCACCCTCCACGGCTGGTGCCGTTCCATTGGCTGGAGTAGCAGTCTCCGGTGGCGGT
GCTGGATAGCACGCGATCTGATCCAACTCAATAGTCTTGGTAGGATCTTTAGGATCCGGACCGGGCACTTTAACGGGCAC
ACAGATGATGCCACCACCAGGTGGTGCATTGGGATCAGCAGGGGCTCCAGGTGCAGGAGGGACTGGCGCGTTTGGATCCG
CCGGATTGGGTGCAACTGCGGGTGCAGGAGCCACAGGAGATCCGGGTGCAACAGGAGCTCCAGAAGCAGATACAGGTGCC
GCTGGATCAACGGGAGCAGGTGGCGATGGACCTAGAGGCGCCAGTGGAACTGATCCTGCAACTGGGGCTGGAGGAACGGG
TAGAGCTTGAGAGTCAGTAACAGGTGGAGCGGCATAAACAGGTGGCACTGGCACAGCTGGAGAAGCGGCTGCTTGGTTTC
TCCAGCATTTATCACCGTAGTACCCGATCCCACATCTGTGGTG
      Library Information (1)
      Library: IP_cDNA
      Description

      Clones isolated by an inverse-PCR screen (SLIP) using a mixture of the GH, LD, LP, and SD cDNA libraries.

      Sample preparation

      Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      These clones have been isolated a directed screening process called SLIP (Self-Ligation of Inverse PCR Products). Clones isolated from this screen have been PCR-amplified using a mixture of 4 different plasmid cDNA libraries as template. These include the GH, LD, LP, and SD cDNA libraries.

      (FlyBase Curators, 2016-)
      Comment
      Reported As
      Symbol Synonyms
      IP
      IP_cDNA
      References (1)
      FlyBase analysis (1)
      FlyBase Curators, 2016-, Curation of large dataset metadata by FlyBase.
      Curation of large dataset metadata by FlyBase. [FBrf0232540]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)