s15, s15-1, s-15
Please see the JBrowse view of Dmel\Cp15 for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.46
0.65 (northern blot)
There is only one protein coding transcript and one polypeptide associated with this gene
115 (aa); 9.5 (kD)
115 (aa)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Cp15 using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Cp15 transcript is first detected in stage S8-S10 of oogenesis, subsequently disappears, and reappears in stage S13-S14.
The six major chorion bands are dramatically reduced in follicles of Mcm6K1214, mus101K451 and fs(1)A1059K1 mutants. No reduction of major chorion bands is seen in swa6 although some affects are seen in higher molecular weight components.
JBrowse - Visual display of RNA-Seq signals
View Dmel\Cp15 in JBrowse3-26
3-20.9
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Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.
Flanking gypsy\su(Hw)BRs can create a chromosomal domain permissible for activity of the chorion gene DNA replication origin, DNA replication is dramatically protected from position effects. Inclusion of only a single gypsy\su(Hw)BR does not detectably protect chorion gene DNA replication origin from position effects.
A 150bp region from -189bp to -39bp encompasses many positive and negative, at least partially degenerate, cis-regulatory elements, which are involved in specifying the highly precise expression of Cp15 during development.
Primarily regulated at the transcriptional level.
Two dimensional gels have been used to map the replication sites within the third chromosome chorion domain during amplification.
The Cp15, Cp16, Cp18 and Cp19 genes of the chorion cluster have been characterized in D.melanogaster, D.subobscura, D.virilis and D.grimshawi. The temporally specific follicular expression of Cp15, Cp16, Cp18 and Cp19 and gene organization has been conserved.
mus101K451, Mcm6K1214 and fs(1)A1059K1 mutants interfere with the amplification of the major chorion genes Cp15, Cp16, Cp18, Cp19, Cp36 and Cp38.
A 73bp segment of the proximal 5' flanking DNA contains sequences essential for the tissue-specific expression and the precise "late" temporal regulation of Cp15. At least three adjacent elements are recognizable within the regulatory sequences, two exerting positive regulation and one negative.
Sequence analysis of Cp15, Cp18 and Cp19 has led to the identification of a specific DNA element that may control tissue specificity of amplification. Sequences have also been found that may recognize DNA-binding proteins and so function in regulating the amplification or expression of the chorion genes.
A specific 3.8kb genomic fragment from the chorion gene cluster at 66D retains the ability to amplify during oogenesis when removed to other locations in the genome.
Probably the locus for which Yannoni and Petri (1980) detected electrophoretic variants by isoelectric focusing.
Encodes S15-1, a chorion protein estimated at 15kD by Waring and Mahowald (1979) and 9.7kD by Petri et al. (1976). Temporal and spatial distribution of expression described by Parks and Spradling (1987).