FlyBase Gene Report: Dmel\CG14443

General Information

Symbol

Dmel\CG14443

Species

D. melanogaster

Name

Annotation Symbol

CG14443

Feature Type

protein_coding_gene

FlyBase ID

FBgn0029880

Gene Model Status

Current

Stock Availability

4 publicly available

Enzyme Name (EC)

RNA helicase (3.6.4.13)

Gene Summary

Probable ATP-binding RNA helicase. (UniProt, Q9W3Y5)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

6C2-6C2

Sequence location

X:6,633,959..6,636,169 [+]

Recombination map

1-18

RefSeq locus

NC_004354 REGION:6633959..6636169

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (9 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR011545

(InterPro Project Members, 2004-)

enables helicase activity

inferred from sequence or structural similarity

(Lasko, 2000)

enables mRNA binding

inferred from biological aspect of ancestor with PANTHER:PTN002776867

(GO Reference Genome Project, 2011-)

enables nucleic acid binding

inferred from electronic annotation with InterPro:IPR011545

(InterPro Project Members, 2004-)

enables RNA helicase activity

inferred from electronic annotation with InterPro:IPR014014

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN002774595

(GO Reference Genome Project, 2011-)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in alternative mRNA splicing, via spliceosome

inferred from biological aspect of ancestor with PANTHER:PTN002773963

(GO Reference Genome Project, 2011-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN002773962

(GO Reference Genome Project, 2011-)

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN002773962

(GO Reference Genome Project, 2011-)

part_of ribonucleoprotein complex

inferred from biological aspect of ancestor with PANTHER:PTN002773962

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

DEAD-BOX RNA HELICASES

Protein Family (UniProt)

Belongs to the DEAD box helicase family. (Q9W3Y5)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

RNA helicase activity

ATP + H2O = ADP + H(+) + phosphate (3.6.4.13)

Summaries

Gene Group (FlyBase)

DEAD-BOX RNA HELICASES -: DEAD-box RNA helicases belong to helicase superfamily 2 (SF2). They are characterized by the presence of nine conserved helicase motifs, including the DEAD sequence in motif II. (Adapted from PMID:21509200.)

Protein Function (UniProtKB)

Probable ATP-binding RNA helicase.

(UniProt, Q9W3Y5)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\CG14443 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9W3Y5)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.39

Gene model reviewed during 5.52

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

retrogene

(dos Santos, 2014, Zhang et al., 2011, Zhang et al., 2010)

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CG14443-RB

FBtr0308654

NM_132102

2211

736

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

CG14443-PB

FBpp0300877

83.1

736

10.42

Q9W3Y5

NP_572330

AAF46176

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

AE014298 / AAF46176

UniProt

Q9W3Y5

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG14443 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG14443_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

3.61

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\CG14443 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CG14443

View exonic expression by tissue for Dmel\CG14443

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31595/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0029880

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0029880

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0029880

Flygut - An atlas of the Drosophila adult midgut

FBgn0029880

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 0 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CG14443

Transgenic constructs containing regulatory region of CG14443

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene disrupted in

Df(1)e02477-d06059

(Casad et al., 2012)

Df(1)Exel6240

(Ryder, 2004.4.26)

Df(1)pigs¹

(Pines et al., 2010)

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CG14443^GD8330

viable, with Scer\GAL4^Mef2.PR

CG14443^GD8330

viable, with Scer\GAL4^pnr-MD237

CG14443^GD8330

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (21)

Hsap\DDX17

4 of 14

Yes

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (18)

Rnor\Ddx5

3 of 14

Yes

Rnor\Ddx17

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (18)

Mmus\Ddx17

4 of 14

Yes

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (9)

Xtro\ddx41

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (11)

Drer\ddx17

4 of 14

Yes

3 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (14)

Cele\ddx-17

3 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (14)

Agam\AgaP_AGAP003663

3 of 12

Yes

Agam\AgaP_AGAP005351

3 of 12

Yes

Agam\AgaP_AGAP005652

3 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (34)

Atha\RH20

4 of 13

Yes

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (9)

Scer\DBP2

4 of 13

Yes

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (9)

Spom\dbp2

4 of 12

Yes

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:CG14443. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (30)

CG10077

4 of 13

mahe

4 of 13

Rm62

4 of 13

abs

3 of 13

bel

3 of 13

CG7878

3 of 13

CG10333

3 of 13

vas

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

ais

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

pit

1 of 13

pths

1 of 13

Rs1

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

DDX17; DEAD-box helicase 17

4 of 14

608469

DDX5; DEAD-box helicase 5

3 of 14

180630

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

MIST Molecular Interaction Search Tool

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

58082

DroID - A comprehensive database of gene and protein interactions.

FBgn0029880

MIST (protein-protein) - An integrated Molecular Interaction Database

31595

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Spliceosome

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-18

Cytogenetic map

6C2-6C2

Sequence location

X:6,633,959..6,636,169 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

6C2-6C2

Limits computationally determined from genome sequence between P{EP}EP1402&P{EP}CG15893^EP1634 and P{EP}Ctr1A^EP1364&P{EP}EP1445

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-18

(FlyBase, 2016)

1-18.6

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (4)

Aberrations including deletions of this gene Aberrations including duplications of this gene

VDRC

v105254

P{KK104272}VIE-260B

v17618

w¹¹¹⁸; P{GD8330}v17618

v318626

PBac{fTRG00633.sfGFP-TVPTBF}VK00033

v341763

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD00888}attP40/CyO-GFP

Genomic Clones (13)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (0)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0029880

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0029880

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Additional comments

The CG14443 gene may have been derived from the CG10777 gene by retroposition.

(Zhang et al., 2011)

The CG14443 gene may have been derived from the CG10777 gene by retroposition sometime after the divergence of the Sophophora and Drosophila subgenera.

(Zhang et al., 2010)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG14443

(Sarov et al., 2016, Bischof and FlyORF project members, 2014.6.20, dos Santos, 2014, Japanese National Institute of Genetics, 2012.5.21, Zhang et al., 2011, Zhang et al., 2011, Pines et al., 2010, Ulvila et al., 2010, Zhang et al., 2010, Zhang et al., 2010, Keleman et al., 2009.8.5, Deddouche et al., 2008, Maines et al., 2007)

cg14443

(Lasko, 2000)

Name Synonyms

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 28 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

31595

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

AAF46176

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9W3Y5

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9W3Y5

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9W3Y5

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31595/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0029880

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0029880

FlyMine - An integrated database for Drosophila genomics

FBgn0029880

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG14443

MARRVEL_MODEL - MARRVEL (model organism gene)

31595

Linkouts

BioGRID - A database of protein and genetic interactions.

58082

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0029880

DroID - A comprehensive database of gene and protein interactions.

FBgn0029880

DRSC - Results frm RNAi screens

FBgn0029880

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG14443_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0029880

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0029880

Flygut - An atlas of the Drosophila adult midgut

FBgn0029880

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Spliceosome

MIST (protein-protein) - An integrated Molecular Interaction Database

31595

References (34)

The following external sites may use different assemblies or annotations than FlyBase.

3.61

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