FlyBase Gene Report: Dmel\ACXA

General Information

Symbol

Dmel\ACXA

Species

D. melanogaster

Name

Adenylyl cyclase X A

Annotation Symbol

CG17176

Feature Type

protein_coding_gene

FlyBase ID

FBgn0040510

Gene Model Status

Current

Stock Availability

6 publicly available

Enzyme Name (EC)

adenylate cyclase (4.6.1.1)

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

Ac62D

Key Links

Genomic Location

Cytogenetic map

34A2-34A2

Sequence location

2L:12,921,091..12,925,453 [+]

Recombination map

2-47

RefSeq locus

NT_033779 REGION:12921091..12925453

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (8 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables adenylate cyclase activity

inferred from biological aspect of ancestor with PANTHER:PTN002899368

(GO Reference Genome Project, 2011-)

inferred from sequence model

(Cann et al., 2000)

Biological Process (5 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in spermatogenesis

inferred from expression pattern

(Cann et al., 2000)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

involved_in adenylate cyclase-activating G protein-coupled receptor signaling pathway

inferred from biological aspect of ancestor with PANTHER:PTN002899368

(GO Reference Genome Project, 2011-)

involved_in cAMP biosynthetic process

inferred from biological aspect of ancestor with PANTHER:PTN002899488

(GO Reference Genome Project, 2011-)

involved_in cyclic nucleotide biosynthetic process

inferred from electronic annotation with InterPro:IPR001054

(InterPro Project Members, 2004-)

involved_in intracellular signal transduction

inferred from electronic annotation with InterPro:IPR001054

(InterPro Project Members, 2004-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in membrane

inferred from sequence model

(Cann et al., 2000)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN002899368

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

ADENYLATE CYCLASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

adenylate cyclase activity

ATP = 3',5'-cyclic AMP + diphosphate (4.6.1.1)

RHEA 15389:

Summaries

Gene Group (FlyBase)

ADENYLATE CYCLASES -: Adenylate cyclases catalyze the synthesis of cAMP from ATP, yielding diphosphate as a by-product. (Adapted from FBrf0238856).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\ACXA for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VK51)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Annotated transcripts may not represent all possible combinations of alternative exons and/or alternative promoters.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Gene model reviewed during 5.53

Multiphase exon postulated: exon reading frame differs in alternative transcripts; overlap >20aa.

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

ACXA-RA

FBtr0080415

NM_138990

3554

1112

ACXA-RC

FBtr0305490

NM_001259082

3539

1107

ACXA-RD

FBtr0305491

NM_001259083

3549

447

ACXA-RG

FBtr0333225

NM_001273494

3567

865

ACXA-RH

FBtr0344132

NM_001298954

3500

394

Additional Transcript Data and Comments

Reported size (kB)

3.555 (longest cDNA)

(Cann et al., 2000)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

ACXA-PA

FBpp0079996

127.9

1112

7.25

FBpp0293943

127.4

1107

7.16

FBpp0293944

52.5

447

8.33

FBpp0305427

100.3

865

8.38

FBpp0310549

46.2

394

7.91

X2JAA1

NP_001285883

AHN54397

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

1112 (aa)

(Cann et al., 2000)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ACXA using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

ACXA_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

2.83

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

spermatogenesis

pre-elongation spermatid

(Cann et al., 2000)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

organism

(Cann et al., 2000)

pupal stage

(Cann et al., 2000)

adult stage | male

(Cann et al., 2000)

adult stage | male & spermatogenesis

spermatocyte

(Cann et al., 2000)

Additional Descriptive Data

Expression is low in late larvae, increases in pupal stages, and is restricted to adult males. Larval and pupal expression is also likely to be restricted to males. During spermatogenesis, expression is detected in postmitotic, meiotic, and early differentiating sperm.

(Cann et al., 2000)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\ACXA in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\ACXA

View exonic expression by tissue for Dmel\ACXA

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

53432/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0040510

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0040510

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0040510

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG17176

Flygut - An atlas of the Drosophila adult midgut

FBgn0040510

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 2 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of ACXA

Transgenic constructs containing regulatory region of ACXA

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2L)Exel7055

(Ryder, 2004.4.26)

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

ACXA^GD16283

viable, with Scer\GAL4^Mef2.PR

ACXA^GD16283

viable, with Scer\GAL4^pnr-MD237

ACXA^GD16283

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (23)

Hsap\ADCY2

4 of 14

Yes

Hsap\ADCY4

4 of 14

Yes

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (23)

Rnor\Adcy2

4 of 14

Yes

Rnor\Adcy4

4 of 14

Yes

Rnor\Adcy7

4 of 14

Yes

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (24)

Mmus\Adcy2

4 of 14

Yes

Mmus\Adcy4

4 of 14

Yes

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (11)

Xtro\adcy4

4 of 13

Yes

3 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\grn

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (26)

Drer\adcy2a

4 of 14

Yes

Drer\adcy2b

4 of 14

Yes

Drer\adcy7

4 of 14

Yes

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Drer\si:ch211-132f19.7

2 of 14

Drer\si:dkey-37g12.1

2 of 14

Drer\si:dkey-206f10.1

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (37)

Cele\acy-2

3 of 14

Yes

Cele\acy-4

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (25)

Agam\AgaP_AGAP007631

7 of 12

Yes

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

Yes

Agam\AgaP_AGAP009087

1 of 12

Agam\AgaP_AGAP012597

1 of 12

Agam\AgaP_AGAP012965

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\CYR1

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\cyr1

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:ACXA. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (27)

9 of 13

9 of 13

9 of 13

8 of 13

7 of 13

6 of 13

Ac3

5 of 13

5 of 13

5 of 13

4 of 13

4 of 13

rut

4 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ADCY2; adenylate cyclase 2

4 of 14

103071

ADCY4; adenylate cyclase 4

4 of 14

600292

ADCY5; adenylate cyclase 5

3 of 14

600293

ADCY6; adenylate cyclase 6

3 of 14

600294

LETHAL CONGENITAL CONTRACTURE SYNDROME 8; LCCS8

ADCY7; adenylate cyclase 7

3 of 14

600385

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Other Interaction Browsers

Summary of Genetic Interactions

esyN Network Diagram

Other Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

72720

DroID - A comprehensive database of gene and protein interactions.

FBgn0040510

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-47

Cytogenetic map

34A2-34A2

Sequence location

2L:12,921,091..12,925,453 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

34A2-34A2

Limits computationally determined from genome sequence between P{lacW}l(2)k07015^k07015&P{PZ}l(2)rK639^rK639 and P{lacW}l(2)k11328^k11328&P{lacW}l(2)k10105^k10105

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

34A-34A

(Cann et al., 2000, Cann, 1999.8.17)

Mapped to P1 clone DS03829, which maps to 34A.

(Cann et al., 2000)

Experimentally Determined Recombination Data

Location

2-47

(FlyBase, 2016)

2-42.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (6)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

30661

y¹ w^*; Mi{MIC}ACXA^MI00200/CyO

86379

y¹ w^*; TI{CRIMIC.TG4.2}ACXA^{CR01427-TG4.2}

62244

y¹ sc^* v¹ sev²¹; P{TRiP.HMC05251}attP40

NIG-Fly

2LG-1704

y² VhaAC39-1^cho-1 v¹; P{U6-ACXA.NIG.sgRNA}attP40/CyO

VDRC

v104283

P{KK107022}VIE-260B

v49100

w¹¹¹⁸; P{GD16283}v49100

Genomic Clones (14)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (3)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

AT28702

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0040510

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0040510

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

"34A" was stated as revision.

(Cann et al., 2000)

Relationship to Other Genes

Source for database merge of

Source for merge of: ACXA CG17176

(Bayraktaroglu, 2000.8.7)

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: ACXA Ac62D

(Cann et al., 2000)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

(Morrison et al., 2000)

AC62D

(Cann et al., 2000)

ACXA

(Noyes and Davis, 2023, Deshpande et al., 2019, Gene Disruption Project members, 2018-, Pathak et al., 2014, Duvall and Taghert, 2013, Duvall and Taghert, 2012, Chen et al., 2010, Ueno and Kidokoro, 2008, Conant and Wagner, 2003)

Ac62D

(Han and Davis, 1995)

CG17176

(National Institute of Genetics Fly Stocks, 2022-, Rumpf et al., 2014, Chen et al., 2012, Zhang et al., 2010, Keleman et al., 2009.8.5, Perkins et al., 2009, Apidianakis et al., 2005, Morrison et al., 2000)

DACXA

(Cann et al., 2000)

Name Synonyms

ACXA

AdenylateCyclase A

(Zhan et al., 2013)

Adenylyl cyclase 62D

Adenylyl cyclase X A

adenylyl cyclase

(Yovell et al., 1992)

Secondary FlyBase IDs

FBgn0004851
FBgn0032460

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 60 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

53432

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VK51

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VK51

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

53432/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0040510

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0040510

FlyMine - An integrated database for Drosophila genomics

FBgn0040510

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG17176

MARRVEL_MODEL - MARRVEL (model organism gene)

53432

Linkouts

BioGRID - A database of protein and genetic interactions.

72720

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0040510

DroID - A comprehensive database of gene and protein interactions.

FBgn0040510

DRSC - Results frm RNAi screens

FBgn0040510

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

ACXA_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0040510

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0040510

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG17176

Flygut - An atlas of the Drosophila adult midgut

FBgn0040510

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0040510

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (46)

The following external sites may use different assemblies or annotations than FlyBase.

2.83

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