FlyBase Gene Report: Dmel\CG42335

General Information

Symbol

Dmel\CG42335

Species

D. melanogaster

Name

Annotation Symbol

CG42335

Feature Type

protein_coding_gene

FlyBase ID

FBgn0259237

Gene Model Status

Current

Stock Availability

5 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

93F9-93F9

Sequence location

3R:21,758,764..21,769,301 [+]

Recombination map

3-73

RefSeq locus

NT_033777 REGION:21758764..21769301

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (9 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables metalloaminopeptidase activity

inferred from biological aspect of ancestor with PANTHER:PTN000164250

(GO Reference Genome Project, 2011-)

enables metallopeptidase activity

inferred from electronic annotation with InterPro:IPR014782

(InterPro Project Members, 2004-)

enables peptide binding

inferred from biological aspect of ancestor with PANTHER:PTN000164250

(GO Reference Genome Project, 2011-)

enables zinc ion binding

inferred from electronic annotation with InterPro:IPR014782

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000164250

(GO Reference Genome Project, 2011-)

Biological Process (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in peptide catabolic process

inferred from biological aspect of ancestor with PANTHER:PTN000164250

(GO Reference Genome Project, 2011-)

involved_in proteolysis

inferred from biological aspect of ancestor with PANTHER:PTN000164251

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR001930

(InterPro Project Members, 2004-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000164250

(GO Reference Genome Project, 2011-)

is_active_in extracellular space

inferred from biological aspect of ancestor with PANTHER:PTN000164250

(GO Reference Genome Project, 2011-)

is_active_in membrane

inferred from biological aspect of ancestor with PANTHER:PTN000164250

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

M1 METALLOPEPTIDASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

M1 METALLOPEPTIDASES -: M1 metallopeptidases belong to MEROPS family M1. All members of the family act on the N-terminus of polypeptides, many of them being aminopeptidases. The family also contains pyroglutamyl-peptidase II, an omega peptidase that releases pyroglutamate from thyrotropin-releasing hormone, and some dipeptidases. (Adapted from FBrf0239469.)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\CG42335 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VD85)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.49

Low-frequency RNA-Seq exon junction(s) not annotated.

Mutation in sequenced strain: insertion of transposon; transcription appears to terminate within the mdg1 transposon.

Gene model reviewed during 5.53

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CG42335-RE

FBtr0344211

NM_169967

2786

924

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

CG42335-PE

FBpp0310619

106.6

924

4.69

Q9VD85

NP_732655

AAF55913

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG42335 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG42335_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.31

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\CG42335 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CG42335

View exonic expression by tissue for Dmel\CG42335

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0259237

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42558/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0259237

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0259237

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0259237

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG31177

Flygut - An atlas of the Drosophila adult midgut

FBgn0259237

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 4 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CG42335

Transgenic constructs containing regulatory region of CG42335

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(3R)Exel6187

(Ryder, 2004.4.26)

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

CG42335^c00239

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CG42335^GD6214

viable, with Scer\GAL4^Mef2.PR

viable, with Scer\GAL4^pnr-MD237

CG42335^GD6214

Sterility

Allele

fertile

CG42335^c00239

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (14)

Hsap\ANPEP

5 of 14

Yes

Hsap\ENPEP

5 of 14

Yes

Hsap\ERAP1

5 of 14

Yes

Hsap\ERAP2

5 of 14

Yes

Hsap\LNPEP

5 of 14

Yes

Hsap\TRHDE

5 of 14

Yes

4 of 14

4 of 14

3 of 14

1 of 14

1 of 14

Yes

Hsap\LTA4H

1 of 14

Hsap\RNPEP

1 of 14

Hsap\RNPEPL1

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (11)

Rnor\Anpep

5 of 14

Yes

Rnor\Enpep

5 of 14

Yes

Rnor\Erap1

5 of 14

Yes

Rnor\Lnpep

5 of 14

Yes

Rnor\Trhde

5 of 14

Yes

4 of 14

4 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (11)

Mmus\Erap1

5 of 14

Yes

Mmus\Lnpep

5 of 14

Yes

Mmus\Trhde

5 of 14

Yes

4 of 14

4 of 14

4 of 14

3 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (10)

Xtro\erap1

5 of 13

Yes

Xtro\lnpep

5 of 13

Yes

Xtro\trhde

5 of 13

Yes

4 of 13

4 of 13

3 of 13

3 of 13

2 of 13

Yes

Xtro\aopep

1 of 13

Xtro\vrk3

1 of 13

Danio rerio (Zebrafish) (17)

Drer\erap1a

6 of 14

Yes

Drer\erap1b

6 of 14

Yes

Drer\erap2

6 of 14

Yes

Drer\enpep

5 of 14

Drer\lnpep

5 of 14

Drer\LOC100004744

5 of 14

Drer\si:ch211-106j24.1

5 of 14

Drer\si:ch211-276a23.5

5 of 14

4 of 14

4 of 14

4 of 14

4 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (14)

Cele\F49B2.6

6 of 14

Yes

5 of 14

5 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

Yes

3 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (24)

Agam\AgaP_AGAP000885

5 of 12

Yes

Agam\AgaP_AGAP003692

5 of 12

Yes

Agam\AgaP_AGAP003695

5 of 12

Yes

Agam\AgaP_AGAP003926

5 of 12

Yes

Agam\AgaP_AGAP004808

5 of 12

Yes

Agam\AgaP_AGAP004860

5 of 12

Yes

Agam\AgaP_AGAP013001

5 of 12

Yes

Agam\AgaP_AGAP013146

5 of 12

Yes

Agam\AgaP_AGAP013188

5 of 12

Yes

Agam\AgaP_AGAP013255

5 of 12

Yes

Agam\AgaP_AGAP013393

5 of 12

Yes

4 of 12

4 of 12

4 of 12

4 of 12

4 of 12

4 of 12

4 of 12

4 of 12

3 of 12

3 of 12

2 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (4)

Atha\APM1

5 of 13

Yes

Atha\AT1G63770

4 of 13

Atha\AT5G13520

1 of 13

Atha\TAF2

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (4)

Scer\AAP1

4 of 13

Yes

Scer\APE2

4 of 13

Yes

Scer\TMA108

3 of 13

Scer\LAP2

1 of 13

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\ape2

4 of 12

Yes

Spom\SPCC1322.05c

1 of 12

Escherichia coli (enterobacterium) (1)

Ecol\pepN

3 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:CG42335. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (21)

6 of 13

6 of 13

6 of 13

6 of 13

6 of 13

6 of 13

6 of 13

6 of 13

6 of 13

6 of 13

Psa

6 of 13

6 of 13

6 of 13

5 of 13

5 of 13

4 of 13

4 of 13

4 of 13

4 of 13

3 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ANPEP; alanyl aminopeptidase, membrane

5 of 14

151530

ENPEP; glutamyl aminopeptidase

5 of 14

138297

ERAP1; endoplasmic reticulum aminopeptidase 1

5 of 14

606832

ERAP2; endoplasmic reticulum aminopeptidase 2

5 of 14

609497

LNPEP; leucyl and cystinyl aminopeptidase

5 of 14

151300

TRHDE; thyrotropin releasing hormone degrading enzyme

5 of 14

606950

LVRN; laeverin

4 of 14

610046

NPEPPS; aminopeptidase puromycin sensitive

4 of 14

606793

NPEPPSP1; NPEPPS pseudogene 1

3 of 14

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0259237

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-73

Cytogenetic map

93F9-93F9

Sequence location

3R:21,758,764..21,769,301 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

93F9-93F9

Limits computationally determined from genome sequence between P{PZ}InR⁰⁵⁵⁴⁵ and P{PZ}tsl⁰⁰⁶¹⁷

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-73

(FlyBase, 2016)

3-74.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (5)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

10032

w¹¹¹⁸; PBac{PB}CG42335^c00239

24208

w¹¹¹⁸; Mi{ET1}CG42335^MB04630

43612

y¹ w^*; Mi{MIC}CG42335^MI07208

VDRC

v106552

P{KK112445}VIE-260B

v108060

P{KK107217}VIE-260B

Genomic Clones (23)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (29)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH14075

Other clones

IP21838

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0259237

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Source for merge of: CG13420 CG31177

(FlyBase Genome Annotators, 2008)

Additional comments

Annotations CG13420 and CG31177 merged as CG42335 in release 5.9 of the genome annotation.

(FlyBase Genome Annotators, 2008)

Annotation CG5845 split into CG31177 and CG31198 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG13420

(Keleman et al., 2009.8.5)

CG31177

(Heinrichsen et al., 2014, Keleman et al., 2009.8.5, Lipatov et al., 2005)

CG42335

(Gupta et al., 2023, Li et al., 2022, Zhou et al., 2021, Blount et al., 2019, Schuster et al., 2013, Sharma et al., 2011, FlyBase Genome Annotators, 2008)

CG5845

(Schulz et al., 2001)

Name Synonyms

CG13420

CG31177

Secondary FlyBase IDs

FBgn0038900
FBgn0051177
FBgn0038899

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 45 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

42558

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VD85

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VD85

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0259237

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42558/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0259237

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0259237

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG42335

MARRVEL_MODEL - MARRVEL (model organism gene)

42558

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0259237

DRSC - Results frm RNAi screens

FBgn0259237

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG42335_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0259237

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0259237

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG31177

Flygut - An atlas of the Drosophila adult midgut

FBgn0259237

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0259237

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (33)

The following external sites may use different assemblies or annotations than FlyBase.

-0.31

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