Human Disease Model Report: cancer, multiple, germline-specific genes

General Information

Name

cancer, multiple, germline-specific genes

FlyBase ID

FBhh0000930

Disease Ontology Term

cancer

Parent Disease

OMIM

Overview

The aberrant expression of germline-specific genes in somatic tumors has been observed in multiple human cancers; these are genes for which normal expression is restricted to male or female germline cells or for which expression is highly biased to germline cells. The products of these genes are of particular interest for targeted therapies, since normal somatic cells are less likely to be impacted.

In Drosophila, screens for loss-of-function suppressors of the malignant brain tumor phenotype of Dmel\l(3)mbt (see the human disease model report 'cancer, neural stem cell, L3MBTL-related' FBhh0000773) have identified multiple germline-specific or germline-biased genes. These include mei-W68 (human SPO11), tud (human TDRD6), nos (human NANOS1, 2, 3), vas (human DDX4), piwi and aub (human PIWIL1, 2, 3, 4).

Drosophila testis-specific paralogs of proteins that translocate nuclearly-encoded mitochondrial proteins into mitochondria have been studied in this context. Translocases of outer membrane (tomboy20, ortholog of human TIMM50) and inner membrane (ttm2, ortholog of human TOMM20 and TOMM20L) complexes have been assessed. Expression in somatic epithelia of either tomboy20 or ttm2 results in tumorigenic phenotypes. In wing imaginal discs, significant overgrowth is observed and appears to be non-autonomous. In comparable experiments using Tom20, the somatic paralog of tomboy20, overgrowth phenotypes are not observed.

[updated Nov. 2020 by FlyBase; FBrf0222196]

Disease Summary Information

Disease Summary: cancer, multiple, germline-specific genes

OMIM report

Human gene(s) implicated

Symptoms and phenotype

Originally described as cancer/testis antigens, the aberrant expression of germline genes in somatic tumors has been observed in multiple human cancers (Feichtinger et al., 2012; pubmed:22918178; Feichtinger et al., 2014; pubmed:24243547; McFarlane et al., 2014; pubmed:25111983).

(FlyBase Curators, 2013-)

Genetics

Cellular phenotype and pathology

Molecular information

External links

Disease synonyms

Related Diseases

Related human health report(s)

cancer, neural stem cell, L3MBTL-related

Ortholog Information

Human gene(s) in FlyBase

Other mammalian ortholog(s) used

D. melanogaster Gene Information (8)

Dmel\mei-W68

Gene Snapshot

meiotic W68 (mei-W68) encodes a protein thought to form a dimer with the TopoVIA subunit encoded by mei-P22 and is required for the formation of double strand breaks during meiosis. [Date last reviewed: 2019-03-14]

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

TOPII

Comments on ortholog(s)

High-scoring ortholog of human SPO11 (1 Drosophila to 1 human). Dmel\mei-W68 shares 32% identity and 53% similarity with the human gene.

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Dmel\tud

Gene Snapshot

Contributions welcome

Molecular function (GO)

methylation-dependent protein binding

Cellular component (GO)

Gene Groups / Pathways

Comments on ortholog(s)

Low- to moderate-scoring ortholog of human TDRD6 and TDRD15 (1 Drosophila to 2 human). Dmel\tud shares 19-20% identity and 34-35% similarity with the human genes.

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Dmel\nanos

Gene Snapshot

nanos (nanos) encodes an RNA-binding protein that forms part of a translational repressor complex. It functions as the localized determinant of abdominal segmentation. It contributes to germline development, germline stem cell renewal, and neuronal morphogenesis and function. [Date last reviewed: 2019-03-14]

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

Comments on ortholog(s)

Moderate-scoring ortholog of human NANOS1, NANOS2, and NANOS3 (1 Drosophila to 3 human). Within a small region defined as the nanos RNA-binding domain, Dmel\nos shares 52-61% identity and 69-72% similarity with the human genes; beyond this region there is little detected homology.

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Dmel\vas

Gene Snapshot

vasa (vas) encodes Vasa (Vas) is a DEAD-box RNA helicase protein. It interacts with eIF5B and promotes translation of grk and mei-P26 mRNAs. Vas also functions in piRNA biogenesis as a component of an Amplifier complex. Maternally-expressed vas is required for oogenesis, transposon silencing in the female germ line, anterior-posterior embryonic patterning, and germ cell specification. [Date last reviewed: 2019-03-21]

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

DEAD

Comments on ortholog(s)

Moderate-scoring ortholog of human DDX4 (1 Drosophila to 1 human). Dmel\vas shares 43% identity and 57% similarity with the human gene.

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Dmel\piwi

Gene Snapshot

Contributions welcome

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

AGO
PICTS

Comments on ortholog(s)

Low- to moderate-scoring ortholog of human PIWIL1, PIWIL3, PIWIL4, and PIWIL2 (3 Drosophila to 4 human). Dmel\piwi shares 31-36% identity and 52-56% similarity with the human genes.

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Dmel\aub

Gene Snapshot

Contributions welcome

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

Comments on ortholog(s)

Moderate-scoring ortholog of human PIWIL1, PIWIL3, PIWIL4, and PIWIL2 (3 Drosophila to 4 human). Dmel\aub shares 31-37% identity and 51-56% similarity with the human genes.

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Dmel\ttm2

Gene Snapshot

tiny tim 2 (ttm2) encodes a mitochondrial inner membrane protein that is involved in mitochondrial protein translocation and maintenance of mitochondrial membrane potential. [Date last reviewed: 2019-03-14]

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

CTD
TIM50F

Comments on ortholog(s)

High-scoring ortholog of human TIMM50 (3 Drosophila to 1 human).

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Dmel\tomboy20

Gene Snapshot

Contributions welcome

Molecular function (GO)

Cellular component (GO)

mitochondrial outer membrane translocase complex

Gene Groups / Pathways

TOMM-T

Comments on ortholog(s)

Moderate-scoring ortholog of human TOMM20 and TOMM20L (2 Drosophila to 2 human).

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Other Genes Used: Viral, Bacterial, Synthetic (0)

Summary of Physical Interactions (209 groups)

tud

protein-protein

Interacting group

Assay

References

tud - AGO3

cosedimentation, western blot, anti bait coimmunoprecipitation, pull down

(Sato et al., 2015, Nishida et al., 2009)

tud - Akt

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

tud - aub

affinity technology, western blot, isothermal titration calorimetry, predetermined participant, pull down, anti tag western blot, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation, Identification by mass spectrometry, x-ray crystallography, autoradiography, cosedimentation, surface plasmon resonance, cross-linking study

(Wahiduzzaman et al., 2024, Huang et al., 2021, Vo et al., 2019, DeHaan et al., 2017, Sato et al., 2015, Creed et al., 2010, Kirino et al., 2010, Liu et al., 2010, Nishida et al., 2009, Thomson et al., 2008)

tud - Dhc64C

anti tag coimmunoprecipitation, cross-linking study, Identification by mass spectrometry

(Zheng et al., 2016)

tud - eIF4A

anti bait coimmunoprecipitation, cross-linking study, Identification by mass spectrometry, anti tag coimmunoprecipitation, anti tag western blot

(Zheng et al., 2016, Thomson et al., 2008)

tud - Eno

anti tag coimmunoprecipitation, cross-linking study, Identification by mass spectrometry

(Zheng et al., 2016)

tud - Gapdh2

anti tag coimmunoprecipitation, cross-linking study, Identification by mass spectrometry

(Zheng et al., 2016)

tud - Khc

anti tag coimmunoprecipitation, cross-linking study, Identification by mass spectrometry

(Zheng et al., 2016)

tud - me31B

anti tag coimmunoprecipitation, anti tag western blot, cross-linking study, Identification by mass spectrometry, anti bait coimmunoprecipitation

(DeHaan et al., 2017, Zheng et al., 2016, Thomson et al., 2008)

tud - osk

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Hurd et al., 2016)

tud - Pyk

anti tag coimmunoprecipitation, cross-linking study, Identification by mass spectrometry, pull down, anti tag western blot

(Wahiduzzaman et al., 2024, Zheng et al., 2016, Gao et al., 2015)

tud - TER94

anti bait coimmunoprecipitation, cross-linking study, Identification by mass spectrometry

(Thomson et al., 2008)

tud - vls

anti tag coimmunoprecipitation, western blot

(Anne and Mechler, 2005)

nanos

protein-protein

Interacting group

Assay

References

nanos - bam

anti tag coimmunoprecipitation, quantitative reverse transcription pcr, western blot

(Malik et al., 2020, Kim et al., 2010)

nanos - brat

anti tag coimmunoprecipitation, quantitative reverse transcription pcr, two hybrid

(Malik et al., 2020, Sonoda and Wharton, 2001)

nanos - bru1

anti tag coimmunoprecipitation, Identification by mass spectrometry, anti tag western blot

(Bansal et al., 2020)

nanos - Cnot4

two hybrid

(Kadyrova et al., 2007)

nanos - cup

pull down, western blot, two hybrid, Identification by mass spectrometry, autoradiography

(Götze et al., 2017, Jeske et al., 2011, Kadyrova et al., 2007, Sonoda and Wharton, 2001, Verrotti and Wharton, 2000)

nanos - DCP2

anti tag coimmunoprecipitation, anti tag western blot

(Raisch et al., 2016)

nanos - mei-P26

anti tag coimmunoprecipitation, western blot, quantitative reverse transcription pcr, anti bait coimmunoprecipitation, primer specific pcr

(Malik et al., 2020, Li et al., 2013, Li et al., 2012)

nanos - Mocs3

anti tag coimmunoprecipitation, anti tag western blot, Identification by mass spectrometry

(Bansal et al., 2020)

nanos - Not1

anti tag coimmunoprecipitation, western blot, pull down, molecular weight estimation by staining, anti tag western blot

(Arvola et al., 2020, Raisch et al., 2016)

nanos - Not3

anti tag coimmunoprecipitation, anti tag western blot, pull down, molecular weight estimation by staining

(Raisch et al., 2016)

nanos - orb

anti tag coimmunoprecipitation, anti tag western blot, Identification by mass spectrometry

(Bansal et al., 2020)

nanos - PAN2

anti tag coimmunoprecipitation, anti tag western blot

(Raisch et al., 2016)

nanos - PAN3

anti tag coimmunoprecipitation, anti tag western blot

(Raisch et al., 2016)

nanos - Patr-1

anti tag coimmunoprecipitation, anti tag western blot

(Raisch et al., 2016)

nanos - pum

x-ray crystallography, rna three hybrid, electrophoretic mobility shift assay, autoradiography, pull down, anti tag western blot, electrophoretic mobility supershift assay, western blot, nucleic acid uv cross-linking assay, anti bait coimmunoprecipitation

(Asaoka et al., 2019, Weidmann et al., 2016, Joly et al., 2013, Menon et al., 2009, Kadyrova et al., 2007, Cho et al., 2006, Edwards et al., 2001, Sonoda and Wharton, 2001, Sonoda and Wharton, 1999)

nanos - Rga

pull down, molecular weight estimation by staining, anti tag coimmunoprecipitation, anti tag western blot, western blot, Identification by mass spectrometry

(Götze et al., 2017, Raisch et al., 2016, Jeske et al., 2011)

nanos - twin

anti tag coimmunoprecipitation, western blot

(Joly et al., 2013)

nanos - wuho

anti tag coimmunoprecipitation, western blot

(Rastegari et al., 2020)

RNA-protein

Interacting group

Assay

References

nanos - AGO1

anti tag coimmunoprecipitation, quantitative reverse transcription pcr, anti bait coimmunoprecipitation, primer specific pcr

(Malik et al., 2020, Pinder and Smibert, 2013)

nanos - aub

iclip, partial RNA sequence identification, anti tag coimmunoprecipitation, quantitative reverse transcription pcr, anti bait coimmunoprecipitation

(Ma et al., 2017, Barckmann et al., 2015, Becalska et al., 2011, Rouget et al., 2010)

nanos - bam

anti tag coimmunoprecipitation, quantitative reverse transcription pcr, western blot

(Malik et al., 2020, Kim et al., 2010)

nanos - bcd

electrophoretic mobility shift assay, autoradiography

(Weidmann et al., 2016)

nanos - bel

pull down, western blot, Identification by mass spectrometry, anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Götze et al., 2017)

nanos - bgcn

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Malik et al., 2020)

nanos - brat

anti tag coimmunoprecipitation, quantitative reverse transcription pcr, two hybrid

(Malik et al., 2020, Sonoda and Wharton, 2001)

nanos - cup

pull down, western blot, two hybrid, Identification by mass spectrometry, autoradiography

(Götze et al., 2017, Jeske et al., 2011, Kadyrova et al., 2007, Sonoda and Wharton, 2001, Verrotti and Wharton, 2000)

nanos - CycB

electrophoretic mobility shift assay, autoradiography

(Weidmann et al., 2016)

nanos - Dhc64C

anti bait coimmunoprecipitation, quantitative reverse transcription pcr

(Lerit and Gavis, 2011)

nanos - egl

anti tag coimmunoprecipitation, quantitative reverse transcription pcr, full identification by DNA sequencing

(Neiswender et al., 2021, Goldman et al., 2019, Vazquez-Pianzola et al., 2017)

nanos - eIF4E1

pull down, western blot

(Jeske et al., 2011)

nanos - eIF4G1

pull down, western blot

(Jeske et al., 2011)

nanos - glo

anti tag coimmunoprecipitation, reverse transcription pcr, nuclear magnetic resonance, electrophoretic mobility shift assay, autoradiography, quantitative reverse transcription pcr

(Warden et al., 2023, Tamayo et al., 2017, Andrews et al., 2011)

nanos - hb

anti tag coimmunoprecipitation, quantitative reverse transcription pcr, x-ray crystallography, rna three hybrid, nucleic acid uv cross-linking assay, autoradiography

(Malik et al., 2019, Weidmann et al., 2016, Edwards et al., 2001, Sonoda and Wharton, 1999)

nanos - Hrb98DE

anti bait coimmunoprecipitation, reverse transcription pcr, anti tag coimmunoprecipitation, nucleic acid uv cross-linking assay, tag visualisation

(Ji and Tulin, 2016)

nanos - lost

pull down, peptide massfingerprinting

(Sinsimer et al., 2011)

nanos - mago

anti tag coimmunoprecipitation, primer specific pcr

(Ghosh et al., 2014)

nanos - Marf1

anti tag coimmunoprecipitation, nucleotide sequence identification, quantitative reverse transcription pcr

(Kawaguchi et al., 2020)

nanos - me31B

pull down, western blot, Identification by mass spectrometry

(Götze et al., 2017, Jeske et al., 2011)

nanos - mei-P26

anti tag coimmunoprecipitation, western blot, quantitative reverse transcription pcr, anti bait coimmunoprecipitation, primer specific pcr

(Malik et al., 2020, Li et al., 2013, Li et al., 2012)

nanos - osk

nucleic acid uv cross-linking assay, anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Jeske et al., 2015)

nanos - Pen

electrophoretic mobility shift assay, autoradiography

(Asaoka et al., 2019)

nanos - Pop2

pull down, western blot, Identification by mass spectrometry

(Götze et al., 2017, Jeske et al., 2011)

nanos - pum

nanos - Rga

pull down, molecular weight estimation by staining, anti tag coimmunoprecipitation, anti tag western blot, western blot, Identification by mass spectrometry

(Götze et al., 2017, Raisch et al., 2016, Jeske et al., 2011)

nanos - rump

anti bait coimmunoprecipitation, primer specific pcr, nucleic acid uv cross-linking assay, autoradiography, pull down, peptide massfingerprinting

(Sinsimer et al., 2011, Jain and Gavis, 2008)

nanos - smg

anti bait coimmunoprecipitation, quantitative reverse transcription pcr, nucleic acid uv cross-linking assay, autoradiography, pull down, Identification by mass spectrometry, coimmunoprecipitation, western blot, anti tag coimmunoprecipitation

(Götze et al., 2017, Chen et al., 2014, Jeske et al., 2011, Rouget et al., 2010, Zaessinger et al., 2006, Semotok et al., 2005, Smibert et al., 1999, Smibert et al., 1996)

nanos - Su(var)205

clip, reverse transcription pcr

(Casale et al., 2019)

nanos - sw

anti bait coimmunoprecipitation, quantitative reverse transcription pcr

(Lerit and Gavis, 2011)

nanos - Sxl

anti bait coimmunoprecipitation, quantitative reverse transcription pcr, primer specific pcr, clip, anti tag coimmunoprecipitation

(Malik et al., 2020, Morita et al., 2018, Li et al., 2013, Chau et al., 2012)

nanos - tral

pull down, western blot, Identification by mass spectrometry

(Götze et al., 2017, Jeske et al., 2011)

nanos - wisp

anti bait coimmunoprecipitation, primer specific pcr

(Cui et al., 2013)

vas

protein-protein

Interacting group

Assay

References

vas - AGO3

anti tag coimmunoprecipitation, cross-linking study, western blot

(Lin et al., 2023)

vas - armi

anti tag coimmunoprecipitation, western blot, Identification by mass spectrometry

(Durdevic and Ephrussi, 2019)

vas - Art1

methyltransferase assay, autoradiography

(Boulanger et al., 2004)

vas - aub

anti tag coimmunoprecipitation, western blot, anti bait coimmunoprecipitation, cross-linking study, Identification by mass spectrometry

(Lin et al., 2023, Durdevic and Ephrussi, 2019, Zheng et al., 2016, Pek and Kai, 2011)

vas - barr

anti tag coimmunoprecipitation, western blot, anti bait coimmunoprecipitation, anti tag western blot

(Pek and Kai, 2011)

vas - bel

anti tag coimmunoprecipitation, Identification by mass spectrometry, western blot

(Durdevic and Ephrussi, 2019)

vas - bru1

pull down, western blot

(Webster et al., 1997)

vas - Cap-D2

anti bait coimmunoprecipitation, western blot

(Pek and Kai, 2011)

vas - CG5726

anti tag coimmunoprecipitation, Identification by mass spectrometry, anti tag western blot

(Bansal et al., 2020)

vas - cup

anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation, western blot, Identification by mass spectrometry

(Bansal et al., 2020, Ottone et al., 2012)

vas - Dcr-1

anti bait coimmunoprecipitation, western blot

(Megosh et al., 2006)

vas - Dhc64C

anti bait coimmunoprecipitation, western blot

(Lerit and Gavis, 2011)

vas - eIF5B

two hybrid, pull down, anti tag western blot

(Dehghani and Lasko, 2016, Johnstone and Lasko, 2004, Carrera et al., 2000)

vas - faf

anti bait coimmunoprecipitation, protein cross-linking with a bifunctional reagent, peptide massfingerprinting

(Liu et al., 2003)

vas - Fmr1

anti bait coimmunoprecipitation, western blot

(Megosh et al., 2006)

vas - Fsn

anti bait coimmunoprecipitation, anti tag western blot

(Kugler et al., 2010)

vas - gus

pull down, molecular weight estimation by staining, two hybrid, isothermal titration calorimetry, predetermined participant, x-ray crystallography, anti bait coimmunoprecipitation, western blot, anti tag western blot

(Dehghani and Lasko, 2016, Kugler et al., 2010, Woo et al., 2006, Woo et al., 2006, Styhler et al., 2002)

vas - Klp10A

coimmunoprecipitation, Identification by mass spectrometry, anti bait coimmunoprecipitation, western blot

(Venkei et al., 2020)

vas - Kots

anti tag coimmunoprecipitation, western blot

(Lim et al., 2022)

vas - me31B

anti tag coimmunoprecipitation, western blot

(McCambridge et al., 2020)

vas - Nop60B

anti tag coimmunoprecipitation, western blot, Identification by mass spectrometry

(Durdevic and Ephrussi, 2019)

vas - orb

anti tag coimmunoprecipitation, anti tag western blot, Identification by mass spectrometry

(Bansal et al., 2020)

vas - osk

pull down, molecular weight estimation by staining, two hybrid, ubiquitin reconstruction, anti tag coimmunoprecipitation, western blot, colocalization, fluorescence microscopy, inferred by author, anti tag western blot, x-ray crystallography, isothermal titration calorimetry, predetermined participant, autoradiography, Identification by mass spectrometry

(Kubíková et al., 2023, Bansal et al., 2020, Jeske et al., 2017, Dehghani and Lasko, 2016, Hurd et al., 2016, Jeske et al., 2015, Wang et al., 2015, Anne, 2010, Suyama et al., 2009, Carrera et al., 2000, Breitwieser et al., 1996)

vas - otu

anti tag coimmunoprecipitation, Identification by mass spectrometry, anti tag western blot

(Bansal et al., 2020)

vas - pAbp

anti tag coimmunoprecipitation, Identification by mass spectrometry, western blot

(Durdevic and Ephrussi, 2019)

vas - piwi

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, cross-linking study

(Lin et al., 2023, Venkei et al., 2020, Megosh et al., 2006)

vas - qin

anti tag coimmunoprecipitation, western blot

(Anand and Kai, 2012)

vas - Rm62

anti tag coimmunoprecipitation, western blot, Identification by mass spectrometry

(Durdevic and Ephrussi, 2019)

vas - spn-E

anti bait coimmunoprecipitation, western blot

(Pek and Kai, 2011)

vas - sw

anti bait coimmunoprecipitation, western blot

(Lerit and Gavis, 2011)

vas - tapas

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, anti tag western blot, pull down, molecular weight estimation by staining

(Jeske et al., 2017, Patil et al., 2014)

vas - tej

pull down, molecular weight estimation by staining, anti tag coimmunoprecipitation, cross-linking study, western blot

(Lin et al., 2023, Jeske et al., 2017, Patil and Kai, 2010)

RNA-protein

Interacting group

Assay

References

vas - mei-P26

electrophoretic mobility shift assay, autoradiography

(Liu et al., 2009)

piwi

protein-protein

Interacting group

Assay

References

piwi - armi

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, affinity chromatography technology, Identification by mass spectrometry, anti tag western blot

(Yamashiro et al., 2020, Munafò et al., 2019, Ilyin et al., 2017, Pandey et al., 2017, Handler et al., 2011, Olivieri et al., 2010, Saito et al., 2010)

piwi - arx

luminescence based mammalian interactome mapping, anti bait coimmunoprecipitation, western blot, molecular sieving, molecular weight estimation by staining, anti tag coimmunoprecipitation, peptide massfingerprinting, pull down, Identification by mass spectrometry

(Mugat et al., 2020, Onishi et al., 2020, Yamaguchi et al., 2020, Dönertas et al., 2013, Ohtani et al., 2013)

piwi - brm

anti bait coimmunoprecipitation, western blot, Identification by mass spectrometry

(Onishi et al., 2020)

piwi - Caf1-55

anti bait coimmunoprecipitation, western blot

(Mugat et al., 2020)

piwi - corto

anti bait coimmunoprecipitation, western blot

(Peng et al., 2016)

piwi - Cp190

anti bait coimmunoprecipitation, western blot

(Moshkovich et al., 2011)

piwi - Dcr-1

anti tag coimmunoprecipitation, western blot

(Megosh et al., 2006)

piwi - E(z)

anti bait coimmunoprecipitation, western blot

(Peng et al., 2016)

piwi - Elys

anti bait coimmunoprecipitation, western blot

(Ilyin et al., 2017)

piwi - esc

anti tag coimmunoprecipitation, anti tag western blot

(Peng et al., 2016)

piwi - Fmr1

anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation, western blot, pull down

(Jiang et al., 2016, Megosh et al., 2006)

piwi - fs(1)Yb

proximity-dependent biotin identification, Identification by mass spectrometry

(Munafò et al., 2021)

piwi - Gasz

anti tag coimmunoprecipitation, western blot

(Yamashiro et al., 2020)

piwi - HDAC1

anti bait coimmunoprecipitation, western blot

(Mugat et al., 2020)

piwi - Hen1

pull down, western blot

(Ohtani et al., 2013)

piwi - His1

pull down, western blot, anti bait coimmunoprecipitation, peptide massfingerprinting, anti tag western blot

(Iwasaki et al., 2016)

piwi - Hsp83

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation

(Gangaraju et al., 2011)

piwi - Kap-α3

anti bait coimmunoprecipitation, anti tag western blot

(Yashiro et al., 2018)

piwi - Klp10A

coimmunoprecipitation, Identification by mass spectrometry, anti bait coimmunoprecipitation, western blot, northern blot

(Venkei et al., 2020, Saito et al., 2009)

piwi - mael

anti bait coimmunoprecipitation, western blot, anti tag western blot

(Onishi et al., 2020)

piwi - MEP-1

anti bait coimmunoprecipitation, western blot

(Mugat et al., 2020)

piwi - Mi-2

anti bait coimmunoprecipitation, Identification by mass spectrometry, western blot

(Mugat et al., 2020, Onishi et al., 2020)

piwi - Nbr

anti tag coimmunoprecipitation, peptide massfingerprinting, anti tag western blot, western blot

(Hayashi et al., 2016, Wang et al., 2016)

piwi - Not1

anti bait coimmunoprecipitation, western blot

(Kordyukova et al., 2020)

piwi - Nup358

anti bait coimmunoprecipitation, western blot, pull down, anti tag western blot

(Parikh et al., 2018)

piwi - nxf2

anti tag coimmunoprecipitation, western blot, anti bait coimmunoprecipitation, Identification by mass spectrometry

(Fabry et al., 2019, Murano et al., 2019)

piwi - Nxt1

anti tag coimmunoprecipitation, western blot

(Fabry et al., 2019)

piwi - Panx

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, Identification by mass spectrometry, coimmunoprecipitation, anti tag western blot

(Onishi et al., 2020, Batki et al., 2019, Fabry et al., 2019, Murano et al., 2019, Sienski et al., 2015, Yu et al., 2015)

piwi - papi

x-ray crystallography, pull down, molecular weight estimation by staining, two hybrid, western blot, anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation, isothermal titration calorimetry, predetermined participant, Identification by mass spectrometry

(Zhang et al., 2018, Liu et al., 2011)

piwi - qin

anti tag coimmunoprecipitation, western blot

(Anand and Kai, 2012)

piwi - shu

anti bait coimmunoprecipitation, western blot

(Olivieri et al., 2012)

piwi - spn-E

anti tag coimmunoprecipitation, cross-linking study, western blot

(Lin et al., 2023)

piwi - squ

anti tag coimmunoprecipitation, anti tag western blot

(Haase et al., 2010)

piwi - Stip1

ion exchange chromatography, molecular sieving, peptide massfingerprinting, anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation

(Gangaraju et al., 2011)

piwi - Su(var)2-10

anti tag coimmunoprecipitation, anti tag western blot, pull down, bimolecular fluorescence complementation, fluorescence microscopy, western blot

(Bence et al., 2024, Mugat et al., 2020, Ninova et al., 2020)

piwi - Su(var)3-3

anti bait coimmunoprecipitation, western blot

(Lepesant et al., 2020)

piwi - Su(var)205

fluorescence polarization spectroscopy, predetermined participant, anti bait coimmunoprecipitation, western blot, nuclear magnetic resonance, two hybrid, clip, reverse transcription pcr

(Casale et al., 2019, Mendez et al., 2013, Mendez et al., 2011, Brower-Toland et al., 2007)

piwi - Su(z)12

anti tag coimmunoprecipitation, anti tag western blot

(Peng et al., 2016)

piwi - Sumo

anti tag coimmunoprecipitation, anti tag western blot

(Ninova et al., 2023)

piwi - tej

anti tag coimmunoprecipitation, cross-linking study, western blot

(Lin et al., 2023)

piwi - thoc5

anti bait coimmunoprecipitation, western blot

(Ilyin et al., 2017)

piwi - Tudor-SN

anti bait coimmunoprecipitation, western blot, ion exchange chromatography, molecular sieving, peptide massfingerprinting

(Ku et al., 2016)

piwi - twin

anti tag coimmunoprecipitation, western blot, anti tag western blot

(Kordyukova et al., 2020)

piwi - U2af38

anti bait coimmunoprecipitation, western blot

(He et al., 2022)

piwi - vas

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, cross-linking study

(Lin et al., 2023, Venkei et al., 2020, Megosh et al., 2006)

piwi - vret

western blot, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation

(Handler et al., 2011, Zamparini et al., 2011)

piwi - xmas

anti bait coimmunoprecipitation, western blot

(Ilyin et al., 2017)

piwi - zuc

anti tag coimmunoprecipitation, anti tag western blot

(Haase et al., 2010)

RNA-protein

Interacting group

Assay

References

piwi - brat

anti bait coimmunoprecipitation, northern blot

(Saito et al., 2009)

piwi - kay

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Klein et al., 2016)

piwi - Klp10A

coimmunoprecipitation, Identification by mass spectrometry, anti bait coimmunoprecipitation, western blot, northern blot

(Venkei et al., 2020, Saito et al., 2009)

piwi - lncRNA:flam

anti bait coimmunoprecipitation, full identification by DNA sequencing, nucleotide sequence identification

(Fagegaltier et al., 2016, Saito et al., 2009)

piwi - Su(var)205

fluorescence polarization spectroscopy, predetermined participant, anti bait coimmunoprecipitation, western blot, nuclear magnetic resonance, two hybrid, clip, reverse transcription pcr

(Casale et al., 2019, Mendez et al., 2013, Mendez et al., 2011, Brower-Toland et al., 2007)

piwi - tj

anti bait coimmunoprecipitation, nucleotide sequence identification, northern blot

(Saito et al., 2009)

aub

protein-protein

Interacting group

Assay

References

aub

blue native page, molecular weight estimation by staining, iclip, partial RNA sequence identification, molecular sieving

(Vo et al., 2019, Ma et al., 2017)

aub - AGO3

anti bait coimmunoprecipitation, western blot, anti tag western blot, cosedimentation, pull down

(Cappucci et al., 2019, Zhang et al., 2011, Rouget et al., 2010, Nishida et al., 2009)

aub - csul

anti tag coimmunoprecipitation, anti tag western blot

(Huang et al., 2021)

aub - Cyp40

proximity-dependent biotin identification, pull down, western blot

(Iki et al., 2023)

aub - Dhc64C

anti tag coimmunoprecipitation, cross-linking study, Identification by mass spectrometry

(Zheng et al., 2016)

aub - eIF3b

affinity technology, tag visualisation by luciferase assay, anti tag coimmunoprecipitation, Identification by mass spectrometry

(Ramat et al., 2020)

aub - eIF3d1

anti tag coimmunoprecipitation, Identification by mass spectrometry, anti tag western blot, affinity technology, tag visualisation by luciferase assay

(Ramat et al., 2020)

aub - eIF3g1

affinity technology, tag visualisation by luciferase assay

(Ramat et al., 2020)

aub - eIF3h

affinity technology, tag visualisation by luciferase assay

(Ramat et al., 2020)

aub - eIF4A

anti tag coimmunoprecipitation, anti tag western blot, cross-linking study, Identification by mass spectrometry

(Ma et al., 2017, Zheng et al., 2016)

aub - eIF4E1

affinity technology, tag visualisation by luciferase assay, anti tag coimmunoprecipitation, anti tag western blot, Identification by mass spectrometry

(Ramat et al., 2020, Ma et al., 2017)

aub - eIF4G1

anti tag coimmunoprecipitation, anti tag western blot

(Ma et al., 2017)

aub - Eno

anti tag coimmunoprecipitation, cross-linking study, Identification by mass spectrometry

(Zheng et al., 2016)

aub - eyg

anti tag coimmunoprecipitation, western blot

(Blom-Dahl and Azpiazu, 2018)

aub - Fmr1

pull down, autoradiography, anti tag coimmunoprecipitation, anti tag western blot

(Bozzetti et al., 2015)

aub - Gapdh2

anti tag coimmunoprecipitation, cross-linking study, Identification by mass spectrometry

(Zheng et al., 2016)

aub - Khc

anti tag coimmunoprecipitation, cross-linking study, Identification by mass spectrometry

(Zheng et al., 2016)

aub - Klp10A

anti bait coimmunoprecipitation, western blot, coimmunoprecipitation, Identification by mass spectrometry

(Venkei et al., 2020)

aub - Kots

anti tag coimmunoprecipitation, western blot

(Lim et al., 2022)

aub - krimp

isothermal titration calorimetry, predetermined participant, anti tag coimmunoprecipitation, anti tag western blot, x-ray crystallography

(Huang et al., 2021)

aub - me31B

anti tag coimmunoprecipitation, cross-linking study, Identification by mass spectrometry

(Zheng et al., 2016)

aub - Not1

anti tag coimmunoprecipitation, western blot

(Rojas-Ríos et al., 2017)

aub - Not3

anti tag coimmunoprecipitation, western blot

(Rojas-Ríos et al., 2017)

aub - p53

pull down, autoradiography

(Lunardi et al., 2010)

aub - pAbp

anti tag coimmunoprecipitation, anti tag western blot, western blot, affinity technology, tag visualisation by luciferase assay, cosedimentation through density gradient, anti bait coimmunoprecipitation, Identification by mass spectrometry, pull down

(Ramat et al., 2020, Ma et al., 2017)

aub - papi

two hybrid, isothermal titration calorimetry, predetermined participant, anti bait coimmunoprecipitation, western blot

(Zhang et al., 2018, Liu et al., 2011)

aub - Pgk

anti tag coimmunoprecipitation, cross-linking study, Identification by mass spectrometry

(Zheng et al., 2016)

aub - Pyk

anti tag coimmunoprecipitation, cross-linking study, Identification by mass spectrometry

(Zheng et al., 2016)

aub - qin

anti tag coimmunoprecipitation, anti tag western blot, western blot

(Anand and Kai, 2012)

aub - rump

anti tag coimmunoprecipitation, western blot

(Becalska et al., 2011)

aub - smg

anti bait coimmunoprecipitation, western blot, iclip, partial RNA sequence identification

(Barckmann et al., 2015, Rouget et al., 2010)

aub - spn-E

anti tag coimmunoprecipitation, cross-linking study, western blot

(Lin et al., 2023)

aub - squ

anti tag coimmunoprecipitation, anti tag western blot

(Pane et al., 2007)

aub - tapas

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, anti tag western blot

(Patil et al., 2014)

aub - tej

anti tag coimmunoprecipitation, cross-linking study, western blot, anti tag western blot

(Lin et al., 2023, Patil and Kai, 2010)

aub - tud

aub - twin

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, anti tag western blot

(Rojas-Ríos et al., 2017, Fu et al., 2015, Rouget et al., 2010)

aub - vas

anti tag coimmunoprecipitation, western blot, anti bait coimmunoprecipitation, cross-linking study, Identification by mass spectrometry

(Lin et al., 2023, Durdevic and Ephrussi, 2019, Zheng et al., 2016, Pek and Kai, 2011)

aub - vls

anti tag coimmunoprecipitation, anti tag western blot

(Huang et al., 2021)

aub - vret

anti tag coimmunoprecipitation, western blot

(Zamparini et al., 2011)

aub - wisp

anti tag coimmunoprecipitation, western blot, anti bait coimmunoprecipitation, pull down, anti tag western blot

(Dufourt et al., 2017)

aub - zuc

anti tag coimmunoprecipitation, anti tag western blot

(Pane et al., 2007)

RNA-protein

Interacting group

Assay

References

aub

blue native page, molecular weight estimation by staining, iclip, partial RNA sequence identification, molecular sieving

(Vo et al., 2019, Ma et al., 2017)

aub - bam

iclip, partial RNA sequence identification

(Ma et al., 2017)

aub - BicC

iclip, partial RNA sequence identification

(Barckmann et al., 2015)

aub - bif

iclip, partial RNA sequence identification

(Barckmann et al., 2015)

aub - brat

iclip, partial RNA sequence identification

(Ma et al., 2017)

aub - bru1

iclip, partial RNA sequence identification

(Barckmann et al., 2015)

aub - Cbl

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Rojas-Ríos et al., 2017)

aub - CG4991

iclip, partial RNA sequence identification

(Barckmann et al., 2015)

aub - cib

iclip, partial RNA sequence identification

(Barckmann et al., 2015)

aub - cnc

iclip, partial RNA sequence identification

(Barckmann et al., 2015)

aub - CycA

iclip, partial RNA sequence identification

(Barckmann et al., 2015)

aub - CycB

anti tag coimmunoprecipitation, quantitative reverse transcription pcr, iclip, partial RNA sequence identification

(Vourekas et al., 2016, Barckmann et al., 2015)

aub - dhd

iclip, partial RNA sequence identification, anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Vourekas et al., 2016, Barckmann et al., 2015)

aub - dnc

iclip, partial RNA sequence identification

(Ma et al., 2017, Barckmann et al., 2015)

aub - dom

iclip, partial RNA sequence identification

(Ma et al., 2017)

aub - gcl

anti tag coimmunoprecipitation, quantitative reverse transcription pcr, iclip, partial RNA sequence identification

(Vourekas et al., 2016, Barckmann et al., 2015)

aub - Hsp83

anti tag coimmunoprecipitation, quantitative reverse transcription pcr, iclip, partial RNA sequence identification

(Vourekas et al., 2016, Barckmann et al., 2015)

aub - Lsd-2

iclip, partial RNA sequence identification

(Barckmann et al., 2015)

aub - mei-P26

iclip, partial RNA sequence identification

(Ma et al., 2017)

aub - nanos

iclip, partial RNA sequence identification, anti tag coimmunoprecipitation, quantitative reverse transcription pcr, anti bait coimmunoprecipitation

(Ma et al., 2017, Barckmann et al., 2015, Becalska et al., 2011, Rouget et al., 2010)

aub - osk

iclip, partial RNA sequence identification, anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Vourekas et al., 2016, Barckmann et al., 2015, Becalska et al., 2011)

aub - pgc

iclip, partial RNA sequence identification

(Barckmann et al., 2015)

aub - put

iclip, partial RNA sequence identification

(Ma et al., 2017)

aub - Rm62

iclip, partial RNA sequence identification

(Ma et al., 2017)

aub - scny

iclip, partial RNA sequence identification

(Ma et al., 2017)

aub - shg

iclip, partial RNA sequence identification

(Ma et al., 2017)

aub - smg

anti bait coimmunoprecipitation, western blot, iclip, partial RNA sequence identification

(Barckmann et al., 2015, Rouget et al., 2010)

aub - spir

iclip, partial RNA sequence identification

(Barckmann et al., 2015)

aub - Sxl

iclip, partial RNA sequence identification

(Ma et al., 2017)

aub - tkv

iclip, partial RNA sequence identification

(Ma et al., 2017)

tomboy20

Alleles Reported to Model Human Disease (Disease Ontology) (15 alleles)

mei-W68

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

mei-W68^KK109695

ameliorates brain cancer

modeled by l(3)mbt^ts1

(Rossi et al., 2017)

tud

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

tud^KK101221

ameliorates brain cancer

modeled by l(3)mbt^ts1

(Rossi et al., 2017)

nanos

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

nanos¹⁷

ameliorates brain cancer

modeled by l(3)mbt^ts1

(Janic et al., 2010)

nanos^KK105687

exacerbates atypical teratoid rhabdoid tumor

modeled by Snr1^KK101602

(Tegeder et al., 2019)

vas

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

vas^PH165

ameliorates brain cancer

modeled by l(3)mbt^ts1

(Janic et al., 2010)

vas^KK100929

ameliorates amyotrophic lateral sclerosis type 6

modeled by Hsap\FUS^UAS.Tag:HA

(Fortuna et al., 2021)

modeled by Hsap\FUS^{R518K.UAS.Tag:HA}

(Fortuna et al., 2021)

piwi

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

piwi^HMS00606

model of primary ovarian insufficiency

CEA

(Gorsi et al., 2022)

Modifiers Based on Experimental Evidence ( 3 )

Allele

Disease

Interaction

References

piwi^HMS00606

exacerbates frontotemporal dementia and/or amyotrophic lateral sclerosis 7

modeled by Hsap\CHMP2B^{Intron5.13xlexAop}

(Fort-Aznar et al., 2020)

piwi¹

ameliorates brain cancer

modeled by l(3)mbt^ts1

(Janic et al., 2010)

exacerbates frontotemporal dementia and/or amyotrophic lateral sclerosis 7

modeled by Hsap\CHMP2B^{Intron5.13xlexAop}

(Fort-Aznar et al., 2020)

piwi²

exacerbates frontotemporal dementia and/or amyotrophic lateral sclerosis 7

modeled by Hsap\CHMP2B^{Intron5.13xlexAop}

(Fort-Aznar et al., 2020)

aub

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 3 )

Allele

Disease

Interaction

References

aub^HN2

ameliorates amyotrophic lateral sclerosis type 6

modeled by caz^VDRC.cUa

(Wakisaka et al., 2019)

aub^QC42

ameliorates amyotrophic lateral sclerosis type 6

modeled by caz^VDRC.cUa

(Wakisaka et al., 2019)

ameliorates brain cancer

modeled by l(3)mbt^ts1

(Janic et al., 2010)

aub^UASp.mGFP6

exacerbates amyotrophic lateral sclerosis type 6

modeled by caz^VDRC.cUa

(Wakisaka et al., 2019)

modeled by caz^HMS00790

(Wakisaka et al., 2019)

ttm2

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

ttm2^{UAS.ORF.GW.Tag:HA}

model of cancer

CEA

(Molnar et al., 2020)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

ttm2^EP3367

ameliorates severe acute respiratory syndrome

modeled by HCoV-SARS\3a^{UAS.CUHK-W1.EGFP}

(Wong et al., 2005)

tomboy20

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

tomboy20^{UAS.ORF.GW.Tag:HA}

model of cancer

CEA

(Molnar et al., 2020)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Alleles Representing Disease-Implicated Variants

Genetic Tools, Stocks and Reagents

Sources of Stocks

Contact lab of origin for a reagent not available from a public stock center.

Bloomington Stock Center Disease Page

Related mammalian, viral, bacterial, or synthetic transgenes

Allele

Transgene

Publicly Available Stocks

Selected Drosophila transgenes

Allele

Transgene

Publicly Available Stocks

ttm2^{UAS.ORF.GW.Tag:HA}

M{UAS-ttm2.ORF.3xHA.GW}

M{UAS-ttm2.ORF.3xHA.GW}ZH-86Fb

aub^UASp.mGFP6

P{UASp-GFP-aub.H}

w^*; P{UASp-GFP-aub.H}8.1

tomboy20^{UAS.ORF.GW.Tag:HA}

M{UAS-tomboy20.ORF.3xHA.GW}

M{UAS-tomboy20.ORF.3xHA.GW}ZH-86Fb

RNAi constructs available

Allele

Transgene

Publicly Available Stocks

tud^HMJ21797

P{TRiP.HMJ21797}

y¹ v¹; P{TRiP.HMJ21797}attP40/CyO

tud^GD14219

P{GD14219}

w¹¹¹⁸; P{GD14219}v24031

y¹ v¹; P{TRiP.GL01172}attP2

tud^HMC03326

P{TRiP.HMC03326}

y¹ v¹; P{TRiP.HMC03326}attP40

ttm2^{lexAop.HMJ23599}

P{lexAop-HMJ23599}

ttm2^GD2232

P{GD2232}

w¹¹¹⁸; P{GD2232}v4075/TM3

y¹ v¹; P{TRiP.HMJ23599}attP40

w¹¹¹⁸; P{GD11831}v30124

y¹ v¹; P{TRiP.JF01390}attP2

aub^HMS00611

P{TRiP.HMS00611}

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00611}attP2

y¹ sc^* v¹ sev²¹; P{TRiP.GL00076}attP2

aub^HMS01945

P{TRiP.HMS01945}

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01945}attP40

w¹¹¹⁸; P{GD11827}v22235

y¹ v¹; P{TRiP.JF01394}attP2/TM3, Ser¹

piwi^HMS00606

M{TRiP.HMS00606}

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00606}attP2

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00185}attP2/TM3, Sb¹

piwi^GL00626

P{TRiP.GL00626}

y¹ sc^* v¹ sev²¹; P{TRiP.GL00626}attP40

y¹ v¹; P{TRiP.HMJ21827}attP40/CyO

tomboy20^GD2899

P{GD2899}

w¹¹¹⁸; P{GD2899}v5985/TM3

y¹ sc^* v¹ sev²¹; P{TRiP.HMS05624}attP40

y¹ v¹; P{TRiP.JF02931}attP2/TM3, Sb¹

nanos^HMS00785

P{TRiP.HMS00785}

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00785}attP2

nanos^HMS00930

P{TRiP.HMS00930}

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00930}attP2

nanos^RNAi.UAS.WIZ

P{UAS-nanos.RNAi.WIZ}

y¹ sc^* v¹ sev²¹; P{TRiP.HM05239}attP2

vas^HMS00431

P{TRiP.HMS00431}

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00431}attP2

vas^HMS00373

P{TRiP.HMS00373}

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00373}attP2

vas^GL01013

P{TRiP.GL01013}

y¹ sc^* v¹ sev²¹; P{TRiP.GL01013}attP40

vas^RNAi.UASp

P{UASp-vas.RNAi}

Selected Drosophila classical alleles

Allele

Allele class

Mutagen

Publicly Available Stocks

ttm2^EP3367

aub^clash

amorphic allele - molecular evidence

Delta2-3 transposase

aub^HN2

ethyl methanesulfonate

aub^HN2 cn¹ bw¹/CyO

aub^QC42

ethyl methanesulfonate

w¹¹¹⁸; aub^QC42 cn¹ bw¹/CyO, P{sevRas1.V12}FK1

piwi^cas9

amorphic allele - molecular evidence

CRISPR/Cas9

piwi¹

P-element activity

w¹¹¹⁸; P{PZ}piwi¹/CyO, P{ActGFP}JMR1

piwi²

P-element activity

w¹¹¹⁸; P{ry11}piwi²/CyO, P{ActGFP}JMR1

piwi^EP1024

loss of function allele

P-element activity

nanos^BN

loss of function allele

P-element activity

w^*; PBac{αTub67C-mCh-bcd-LEXY(FRT.bcd3'UTR)sqh3'UTR}VK00033 bcd⁶ nanos^BN e¹ tsl⁴/TM3, Sb¹

nanos¹⁷

nanos¹⁸

amorphic allele - genetic evidence

ethyl methanesulfonate

nanos⁵³

loss of function allele

ethyl methanesulfonate

nanos^L7

loss of function allele

ethyl methanesulfonate

st¹ pum⁶⁸⁰ nanos^L7 e¹

vas^LYG2

amorphic allele - genetic evidence

P-element activity

vas^P808

amorphic allele - genetic evidence

PM hybrid dysgenesis

vas³

loss of function allele

ethyl methanesulfonate

vas⁷

loss of function allele

ethyl methanesulfonate

vas¹

amorphic allele - genetic evidence

ethyl methanesulfonate

vas¹ cn¹ bw¹/CyO

vas^PH165

amorphic allele - molecular evidence

ethyl methanesulfonate

References (6)

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