FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
wds, NC2β
FlyBase ID
FBig0000001965
Interaction Network
Interactions Browser links
wds network
NC2β network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0204126-64.coIP.MS
Reference
Suganuma et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
wds 

WDS

bait

HA tag, FLAG tag

NC2β 

NC2β

prey

5 unique peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed in stably transfected cell lines.

(Suganuma et al., 2008)

Prey endogenous to embryonic nuclear extract.

(Suganuma et al., 2008)
FBrf0214172-59.coIP.MS
Reference
Suganuma et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
wds 

WDS

bait

FLAG tag, HA tag

NC2β 

NC2 beta

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Suganuma et al., 2010)

Tandem affinity purification of bait and identification of prey by MudPIT.

(Suganuma et al., 2010)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)