FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
drosha, pasha
FlyBase ID
FBig0000003447
Interaction Network
Interactions Browser links
drosha network
pasha network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0180557-4.coIP.WB
Reference
Denli et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
pasha 

Pasha

bait
drosha 

Drosha

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The drosha-pasha interaction is not disrupted by RNase treatment.

(Denli et al., 2004)

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Denli et al., 2004)
FBrf0180557-5.coIP.WB
Reference
Denli et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
drosha 

Drosha

bait
pasha 

Pasha

prey

T7 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from transfected construct.

(Denli et al., 2004)
FBrf0180557-7.CH.WB
Reference
Denli et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
drosha 

Drosha

neutral component
drosha 

Drosha

neutral component
drosha 

Drosha

neutral component
pasha 

Pasha

neutral component
pasha 

Pasha

neutral component
pasha 

Pasha

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

S2 cell extracts were fractionated by gel filtration, Q sepharose and phenyl HP chromatography. Pre-miRNA generating activity was tracked during fractionation.

(Denli et al., 2004)

drosha and pasha cofractionate as part of a 500 kDa Microprocessor complex with pre-miRNA generating activity.

(Denli et al., 2004)

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Denli et al., 2004)
FBrf0224009-1.coIP.WB
Reference
Luhur et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
drosha 

Drosha

bait

FLAG tag

pasha 

Pasha

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Kc167 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Luhur et al., 2014)

Positive control.

(Luhur et al., 2014)
FBrf0228549-1.coIP.WB
Reference
Mugat et al., 2015
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
drosha 

drosha

bait

FLAG tag

pasha 

pasha

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Mugat et al., 2015)
FBrf0229448-8.coIP.WB
Reference
Xiong et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
drosha 

Drosha

bait

T7 tag

drosha 
pasha 

Pasha

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
drosha 
c-terminal fragment
sufficient binding region
aa 334-642
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Xiong et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)