FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Mocs2B, wds
FlyBase ID
FBig0000013267
Interaction Network
Interactions Browser links
Mocs2B network
wds network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0204126-34.coIP.MS
Reference
Suganuma et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Mocs2B 

CG10238

bait

HA tag, FLAG tag

wds 

WDS

prey

12 unique peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed in stably transfected cell lines.

(Suganuma et al., 2008)

Prey endogenous to embryonic nuclear extract.

(Suganuma et al., 2008)
FBrf0204126-60.coIP.MS
Reference
Suganuma et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
wds 

WDS

bait

HA tag, FLAG tag

Mocs2B 

CG10238

prey

16 unique peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed in stably transfected cell lines.

(Suganuma et al., 2008)

Prey endogenous to embryonic nuclear extract.

(Suganuma et al., 2008)
FBrf0214172-12.coIP.MS
Reference
Suganuma et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Mocs2B 

CG10238

bait

FLAG tag, HA tag

Mocs2B 
Mocs2B 
wds 

WDS

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mocs2B 
C-terminal region
sufficient binding region
aa 137-367
Mocs2B 
N-terminal region
sufficient binding region
aa 1-137
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Suganuma et al., 2010)

The Mocs2 C-terminal region coimmunoprecipitates wds more efficiently than the Mocs2 N-terminal region.

(Suganuma et al., 2010)

Tandem affinity purification of bait and identification of prey by MudPIT.

(Suganuma et al., 2010)
FBrf0214172-52.coIP.MS
Reference
Suganuma et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
wds 

WDS

bait

FLAG tag, HA tag

Mocs2B 

CG10238

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Suganuma et al., 2010)

Tandem affinity purification of bait and identification of prey by MudPIT.

(Suganuma et al., 2010)
FBrf0214172-84.coIP.WB
Reference
Suganuma et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Mocs2B 

CG10238

bait

FLAG tag, HA tag

Mocs2B 
wds 

WDS

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mocs2B 
C-terminal region
sufficient binding region
aa 137-367
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Suganuma et al., 2010)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)