FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Mfap1, Prp38
FlyBase ID
FBig0000031907
Interaction Network
Interactions Browser links
Mfap1 network
Prp38 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0207382-1.coIP.MS
Reference
Andersen and Tapon, 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Prp38 

dPrp38

bait
Mfap1 

dMFAP1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Immunoprecipitation samples run on SDS-PAGE gel; unique bands (experimental vs control) were excised and subjected to mass spectrometry.

(Andersen and Tapon, 2008)
FBrf0207382-2.coIP.MS
Reference
Andersen and Tapon, 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Mfap1 

dMFAP1

bait
Prp38 

dPrp38

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Immunoprecipitation samples run on SDS-PAGE gel; unique bands (experimental vs control) were excised and subjected to mass spectrometry.

(Andersen and Tapon, 2008)
FBrf0207382-3.coIP.WB
Reference
Andersen and Tapon, 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Prp38 

dPrp38

bait
Mfap1 

dMFAP1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0207382-4.coIP.WB
Reference
Andersen and Tapon, 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Mfap1 

dMFAP1

bait
Prp38 

dPrp38

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0207382-5.PD.WB
Reference
Andersen and Tapon, 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mfap1 

dMFAP1

bait

GST pull down

Prp38 

dPrp38

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Cloned reagents; interaction in solution.

(Andersen and Tapon, 2008)
FBrf0207382-6.PD.WB
Reference
Andersen and Tapon, 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Prp38 

dPrp38

bait

His pull down

Mfap1 

dMFAP1

prey

GST

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Cloned reagents; interaction in solution.

(Andersen and Tapon, 2008)

Cleaved GST-Mfap1 fusion construct used as prey; anti-Mfap1 antibody used for western blot.

(Andersen and Tapon, 2008)
FBrf0207382-7.coIP.WB
Reference
Andersen and Tapon, 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Mfap1 

dMFAP1

bait

HA tag

Mfap1 
Prp38 

dPrp38

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mfap1 
C terminal
unspecified role
aa 229-478
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell lines transfected with tagged Mfap1 constructs.

(Andersen and Tapon, 2008)
FBrf0207382-8.coIP.WB
Reference
Andersen and Tapon, 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Prp38 

dPrp38

bait

HA tag

Prp38 
Mfap1 

dMFAP1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Prp38 
region
unspecified role
aa 1-266aa 181-266

necessary for high level interaction

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell lines transfected with tagged Prp38 constructs.

(Andersen and Tapon, 2008)
FBrf0218395-10915.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mfap1 
unspecified role
Mfap1 
unspecified role
Mfap1 
unspecified role
Prp38 
unspecified role
Prp38 
unspecified role
Prp38 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 359.71647

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)