Interaction in vitro; proteins derived from S2 nuclear extract.
These proteins coimmunoprecipitate with Ada2a and cofractionate with each other in size exclusion chromatography as part of 1.3-1.8 MDa ATAC histone acetyltransferase complex.
TAP-tagged Ada2a was affinity purified and co-purifying proteins were analyzed by gel filtration.
Bait expressed in stably transfected cell lines.
Prey endogenous to embryonic nuclear extract.
Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Tandem affinity purification of bait and identification of prey by MudPIT.
Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Tandem affinity purification of bait and identification of prey by MudPIT.
Tagged complexes were isolated by affinity purification and separated by size exclusion chromatography before mass spectrometry.
Interact as part of the ATAC histone acetyltransferase complex.
Bait expressed in stably transfected cells.
Prey endogenous to nuclear extract.
HGScore = 129.69695