FB2024_03 , released June 25, 2024
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
Chrac-14, Hcf
FlyBase ID
FBig0000070094
Interaction Network
Interactions Browser links
Chrac-14 network
Hcf network
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0204126-39.coIP.MS
Reference
Suganuma et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Chrac-14 

CHRAC14

bait

HA tag, FLAG tag

Hcf 

HCF

prey

47 unique peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed in stably transfected cell lines.

(Suganuma et al., 2008)

Prey endogenous to embryonic nuclear extract.

(Suganuma et al., 2008)
FBrf0214172-36.coIP.MS
Reference
Suganuma et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Chrac-14 

CHRAC14

bait

FLAG tag, HA tag

Hcf 

HCF

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Suganuma et al., 2010)

Tandem affinity purification of bait and identification of prey by MudPIT.

(Suganuma et al., 2010)
FBrf0218395-97.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Hcf 
unspecified role
Hcf 
unspecified role
Hcf 
unspecified role
Chrac-14 
unspecified role
Chrac-14 
unspecified role
Chrac-14 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 130.29888

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)