Interaction in vitro; proteins derived from S2 nuclear extract.
These proteins coimmunoprecipitate with Ada2a and cofractionate with each other in size exclusion chromatography as part of 1.3-1.8 MDa ATAC histone acetyltransferase complex.
TAP-tagged Ada2a was affinity purified and co-purifying proteins were analyzed by gel filtration.
Bait expressed in stably transfected cell lines.
Prey endogenous to embryonic nuclear extract.
Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Tandem affinity purification of bait and identification of prey by MudPIT.
Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Tandem affinity purification of bait and identification of prey by MudPIT.
Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Bait expressed in stably transfected cells.
Prey endogenous to nuclear extract.
HGScore = 215.53822
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
