FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
su(Hw), mod(mdg4)
FlyBase ID
FBig0000071265
Interaction Network
Interactions Browser links
su(Hw) network
mod(mdg4) network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0083144-1.PD.WB
Reference
Gerasimova et al., 1995
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mod(mdg4) 

Mod(mdg4)

bait

GST tag

su(Hw) 

su(Hw)

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from cell line nuclear extract.

(Gerasimova et al., 1995)
FBrf0136690-1.Y2H
Reference
Ghosh et al., 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
su(Hw) 

Su(Hw)

bait

fused to GAL4 DNA-binding domain

su(Hw) 
su(Hw) 
su(Hw) 
mod(mdg4) 

Mod(mdg4) 2.2

prey

fused to GAL4 activation domain

mod(mdg4) 
mod(mdg4) 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
su(Hw) 
leucine zipper and conserved regions B and C
sufficient binding region
aa 760-860
su(Hw) 
leucine zipper
necessary binding region
aa 760-778
su(Hw) 
conserved regions B and C
necessary binding region
aa 779-860
mod(mdg4) 
C-terminal acidic region
sufficient binding region
mod(mdg4) 
C-terminal acidic region
necessary binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Ghosh et al., 2001)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Ghosh et al., 2001)
FBrf0136690-2.Y2H
Reference
Ghosh et al., 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mod(mdg4) 

Mod(mdg4) 2.2

bait

fused to GAL4 DNA-binding domain

su(Hw) 

Su(Hw)

prey

fused to GAL4 activation domain

su(Hw) 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
su(Hw) 
leucine zipper and conserved regions B and C
sufficient binding region
aa 760-860
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Ghosh et al., 2001)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Ghosh et al., 2001)
FBrf0137040-1.PD.A
Reference
Gause et al., 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
su(Hw) 

Su(Hw)

bait

GST tag

su(Hw) 
su(Hw) 
mod(mdg4) 

Mod(mdg4)-67.2

prey

[35]S label

mod(mdg4) 
mod(mdg4) 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
su(Hw) 
enhancer blocking domain
sufficient binding region
aa 693-880
su(Hw) 
enhancer blocking domain
necessary binding region
aa 738-780aa 782-944aa 853-880
mod(mdg4) 
C-terminal region
sufficient binding region
aa 308-610
mod(mdg4) 
C-terminal region
necessary binding region
aa 567-610
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Gause et al., 2001)
FBrf0180528-7.Y2H
Reference
Pai et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
su(Hw) 

Su(Hw)

bait

fused to GAL4 DNA-binding domain

mod(mdg4) 

Mod(mdg4)2.2

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Pai et al., 2004)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Pai et al., 2004)
FBrf0191177-4.Y2H
Reference
Capelson and Corces, 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mod(mdg4) 

Mod(mdg4)2.2

bait

fused to LexA DNA-binding domain

su(Hw) 

Su(Hw)

prey

fused to VP16 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/VP16-AD

(Capelson and Corces, 2005)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Capelson and Corces, 2005)
FBrf0192714-2.Y2H
Reference
Golovnin et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mod(mdg4) 

Mod(mdg4)

bait

fused to GAL4 DNA-binding domain

su(Hw) 

Su(Hw)

prey

fused to GAL4 activation domain

su(Hw) 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
su(Hw) 
mod(mdg4) interacting domain
sufficient binding region
aa 672-889
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD.

(Golovnin et al., 2007)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Golovnin et al., 2007)
FBrf0192714-3.Y2H
Reference
Golovnin et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
su(Hw) 

Su(Hw)

bait

fused to GAL4 DNA-binding domain

su(Hw) 
mod(mdg4) 

Mod(mdg4)

prey

fused to GAL4 activation domain

mod(mdg4) 
mod(mdg4) 
mod(mdg4) 
mod(mdg4) 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
su(Hw) 
mod(mdg4) interacting domain
sufficient binding region
aa 672-889
mod(mdg4) 
BTB domain residues 33 and 46
mutation decreasing interaction
aa 33,46

D33N, H46D. Coordinates relative to mod(mdg4)-PT

mod(mdg4) 
BTB domain residue 46
mutation decreasing interaction
aa 46

H46D, Coordinates relative to mod(mdg4)-PT

mod(mdg4) 
BTB domain residue 47
mutation decreasing interaction
aa 47

R47Q, coordinates relative to mod(mdg4)-PT

mod(mdg4) 
BTB domain residue 33
mutation decreasing interaction
aa 33

D33N, coordinates relative to mod(mdg4)-PT

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Golovnin et al., 2007)

Two-hybrid system: yeast GAL4-BD/GAL4-AD.

(Golovnin et al., 2007)
FBrf0205475-1.Y2H
Reference
Golovnin et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mod(mdg4) 

Mod

bait

fused to GAL4 DNA-binding domain

mod(mdg4) 
mod(mdg4) 
su(Hw) 

Su(Hw)

prey

fused to GAL4 activation domain

su(Hw) 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
su(Hw) 
Mod(mdg4)-67.2-interacting domain
sufficient binding region

"MID" construct

mod(mdg4) 
C-terminal region
necessary binding region
aa 568-610

"ΔC" construct, coordinates relative to mod(mdg4)-PT isoform

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Golovnin et al., 2008)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Golovnin et al., 2008)
FBrf0205475-2.Y2H
Reference
Golovnin et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
su(Hw) 

Su(Hw)

bait

fused to GAL4 activation domain

su(Hw) 
mod(mdg4) 

Mod

prey

fused to GAL4 DNA-binding domain

mod(mdg4) 
mod(mdg4) 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
su(Hw) 
Mod(mdg4)-67.2-interacting domain
sufficient binding region

"MID" construct

mod(mdg4) 
C-terminal region
necessary binding region
aa 568-610

"ΔC" construct, coordinates relative to mod(mdg4)-PT isoform

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Golovnin et al., 2008)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Golovnin et al., 2008)
FBrf0205475-4.coIP.WB
Reference
Golovnin et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
su(Hw) 

Su(Hw)

bait
mod(mdg4) 

Mod

prey

FLAG tag

mod(mdg4) 
mod(mdg4) 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mod(mdg4) 
C-terminal region
necessary binding region
aa 568-610

"ΔC" construct, coordinates relative to mod(mdg4)-PT isoform

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from transfected construct.

(Golovnin et al., 2008)

The mod(mdg4) C-terminal region of the "67.2" isoform is necessary for full coimmunoprecipitation with su(Hw). C-terminal truncated mod(mdg4) coimmunoprecipitates with su(Hw) at reduced levels, presumably through oligomerization with wildtype mod(mdg4) in the cell.

(Golovnin et al., 2008)
FBrf0218398-1.coIP.WB
Reference
Golovnin et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
su(Hw) 

Su(Hw)

bait
mod(mdg4) 

Mod(mdg4)-67.2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Golovnin et al., 2012)
FBrf0218398-3.coIP.WB
Reference
Golovnin et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
mod(mdg4) 

Mod(mdg4)-67.2

bait
su(Hw) 

Su(Hw)

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Golovnin et al., 2012)
FBrf0218724-2.EMSA.A
Reference
Soshnev et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
su(Hw) 

Su(Hw)

neutral component

His tag

su(Hw) 

Su(Hw)

neutral component

His tag

su(Hw) 

Su(Hw)

neutral component

His tag

mod(mdg4) 
mod(mdg4) 

mod(mdg4)

neutral component

His tag

mod(mdg4) 

mod(mdg4)

neutral component

His tag

mod(mdg4) 
mod(mdg4) 

mod(mdg4)

neutral component

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Soshnev et al., 2012)

su(Hw) and Cp190 were mixed with [32]P labeled DNA. DNA-protein complexes observed by EMSA were used to deduce protein-protein interaction.

(Soshnev et al., 2012)

mod(mdg4) does not bind DNA on its own; when mixed with su(Hw), a supershift forms. Addition of Cp190 results in a more pronounced supershift.

(Soshnev et al., 2012)
FBrf0220170-2.PD.WB
Reference
Matzat et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
su(Hw) 

Su(Hw)

bait

GST tag

mod(mdg4) 

Mod(mdg4)2.2

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

(Matzat et al., 2012)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Matzat et al., 2012)
FBrf0220170-4.PD.WB
Reference
Matzat et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mod(mdg4) 

Mod(mdg4)2.2

bait

GST tag

su(Hw) 

Su(Hw)

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

(Matzat et al., 2012)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Matzat et al., 2012)
FBrf0223771-3.coIP.WB
Reference
Kyrchanova et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
su(Hw) 

Su(Hw)

bait
mod(mdg4) 

Mod

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kyrchanova et al., 2013)
FBrf0223771-4.coIP.WB
Reference
Kyrchanova et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
mod(mdg4) 

Mod

bait
su(Hw) 

Su(Hw)

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kyrchanova et al., 2013)
FBrf0234617-3.Y2H
Reference
Golovnin et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
su(Hw) 

Su(Hw)

bait

fused to GAL4 DNA-binding domain

su(Hw) 
mod(mdg4) 

Mod(mdg4)67.2

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
su(Hw) 
C-terminal region
sufficient binding region
aa 672-941
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Golovnin et al., 2016)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Golovnin et al., 2016)
FBrf0236960-1.Y2H
Reference
Melnikova et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
su(Hw) 

Su(Hw)

bait

fused to GAL4 DNA-binding domain

su(Hw) 
su(Hw) 
mod(mdg4) 

Mod-67.2

prey

fused to GAL4 activation domain

mod(mdg4) 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
su(Hw) 
LZ domain
sufficient binding region
aa 716-892
su(Hw) 
N-terminal domain
sufficient binding region
aa 1-238

Interaction only observed when GAL4 fused to C-terminus of su(Hw)

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Melnikova et al., 2017)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Melnikova et al., 2017)
FBrf0236960-2.Y2H
Reference
Melnikova et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mod(mdg4) 

Mod-67.2

bait

fused to GAL4 DNA-binding domain

mod(mdg4) 
mod(mdg4) 
mod(mdg4) 
mod(mdg4) 
su(Hw) 

Su(Hw)

prey

fused to GAL4 activation domain

su(Hw) 
su(Hw) 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
su(Hw) 
LZ domain
sufficient binding region
aa 672-892
su(Hw) 
N-terminal domain
sufficient binding region
aa 1-238
mod(mdg4) 
C-terminal domain
sufficient binding region
aa 568-610

coordinates relative to mod(mdg4)-PT

mod(mdg4) 
N-terminal domain
sufficient binding region
aa 1-276

coordinates relative to mod(mdg4)-PT

mod(mdg4) 
Q domain
necessary binding region
aa 119-276

coordinates relative to mod(mdg4)-PT

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Melnikova et al., 2017)

Two-hybrid system: yeast GAL4-BD/GAL4-AD, mod(mdg4)-PT C-terminal domain, aa 568-610, sufficient for binding su(Hw) LZ domain, aa 672-892. mod(mdg4)-PT Q domain, aa 119-276, necessary for binding su(Hw) N-terminal domain, aa 1-238.

(Melnikova et al., 2017)
FBrf0236960-3.PD.WB
Reference
Melnikova et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mod(mdg4) 

Mod-67.2

bait

GST tag

mod(mdg4) 
su(Hw) 

Su(Hw)

prey

His tag

su(Hw) 
su(Hw) 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
su(Hw) 
C-terminal domain
sufficient binding region
aa 716-945
su(Hw) 
LZ domain
necessary binding region
aa 716-779
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Melnikova et al., 2017)
FBrf0236960-4.PD.WB
Reference
Melnikova et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
su(Hw) 

Su(Hw)

bait

GST tag

su(Hw) 
su(Hw) 
mod(mdg4) 

Mod-67.2

prey

His tag

mod(mdg4) 
mod(mdg4) 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
su(Hw) 
C-terminal domain
sufficient binding region
aa 716-945

with full-length mod(mdg4)-PT as prey

su(Hw) 
N-terminal domain
sufficient binding region
aa 1-238
mod(mdg4) 
Q domain
sufficient binding region
aa 118-277

coordinates relative to mod(mdg4)-PT

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Melnikova et al., 2017)
FBrf0236960-7.coIP.WB
Reference
Melnikova et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
su(Hw) 

Su(Hw)

bait

FLAG tag

su(Hw) 
mod(mdg4) 

Mod-67.2

prey
mod(mdg4) 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
su(Hw) 
N-terminal domain
sufficient binding region
aa 1-238
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Melnikova et al., 2017)
FBrf0236960-9.coIP.WB
Reference
Melnikova et al., 2017
Description
physical association
Assay
Collection
Source/Stage
organism / maleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
su(Hw) 

Su(Hw)

bait

FLAG tag

su(Hw) 
mod(mdg4) 

Mod-67.2

prey
mod(mdg4) 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
su(Hw) 
C-terminal domain
sufficient binding region
aa 238-945
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult males of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Melnikova et al., 2017)
FBrf0238173-6.coIP.WB
Reference
Melnikova et al., 2018
Description
physical association
Assay
Collection
Source/Stage
organism / maleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
su(Hw) 

Su(Hw)

bait

FLAG tag

su(Hw) 
mod(mdg4) 

Mod-67.2

prey
mod(mdg4) 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
su(Hw) 
N-terminal domain
sufficient binding region
aa 1-88,202-238
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult males of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Melnikova et al., 2018)
FBrf0238201-2.Y2H
Reference
Melnikova et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
su(Hw) 

Su(Hw)

unspecified role
su(Hw) 

Su(Hw)

unspecified role
su(Hw) 
su(Hw) 

Su(Hw)

unspecified role
su(Hw) 
mod(mdg4) 

Mod-67.2

unspecified role
mod(mdg4) 

Mod-67.2

unspecified role
mod(mdg4) 

Mod-67.2

unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
su(Hw) 
N-terminal domain
sufficient binding region
aa 1-892
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD.

(Melnikova et al., 2018)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Melnikova et al., 2018)
FBrf0238201-4.coIP.WB
Reference
Melnikova et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
su(Hw) 

Su(Hw)

bait

FLAG tag

mod(mdg4) 

Mod-67.2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Melnikova et al., 2018)
FBrf0238201-6.coIP.WB
Reference
Melnikova et al., 2018
Description
physical association
Assay
Collection
Source/Stage
organism / maleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
su(Hw) 

Su(Hw)

bait

FLAG tag

mod(mdg4) 

Mod-67.2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult males of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Melnikova et al., 2018)
FBrf0241724-5.coIP.WB
Reference
Bag et al., 2019
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
su(Hw) 

Su(Hw)

bait
mod(mdg4) 

Mod(mdg4)67.2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Bag et al., 2019)
FBrf0243085-3.coIP.WB
Reference
Kostyuchenko et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
mod(mdg4) 

Mod(mdg4)

bait

FLAG tag

mod(mdg4) 
su(Hw) 

Su(Hw)

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mod(mdg4) 
C-terminal domain
sufficient binding region
aa 389-610

coordinates relative to mod(mdg4)-PT

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Kostyuchenko et al., 2019)
FBrf0244288-8.PD.WB
Reference
Melnikova et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
su(Hw) 

Su(Hw)

bait

GST tag

su(Hw) 
mod(mdg4) 

Mod-67.2

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
su(Hw) 
LZ domain
sufficient binding region
aa 720-892
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Melnikova et al., 2019)
FBrf0249435-13.coIP.MS
Reference
Bag et al., 2021
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
su(Hw) 

Su(Hw)

bait
mod(mdg4) 

Mod(mdg4)67.2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Bag et al., 2021)
FBrf0249435-2.coIP.WB
Reference
Bag et al., 2021
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
su(Hw) 

Su(Hw)

bait
mod(mdg4) 

Mod(mdg4)67.2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Bag et al., 2021)
FBrf0249435-8.coIP.WB
Reference
Bag et al., 2021
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mod(mdg4) 

Mod(mdg4)67.2

bait
su(Hw) 

Su(Hw)

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Bag et al., 2021)
FBrf0253430-13.PD.MS
Reference
Kaushal et al., 2022
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
su(Hw) 

Su(Hw)

bait

GFP tag

su(Hw) 
mod(mdg4) 

mod(mdg4)

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
su(Hw) 
N terminal domain
sufficient binding region
aa 1-219

coordinates relative to su(Hw)-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type, nuclear embryonic extract.

(Kaushal et al., 2022)
FBrf0254231-8.coIP.MS
Reference
Chen et al., 2022
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
su(Hw) 

su(Hw)

bait
mod(mdg4) 

mod(mdg4)

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extracts of a wild type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Chen et al., 2022)
FBrf0257791-13.Y2H
Reference
Melnikova et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mod(mdg4) 

Mod(mdg4)

bait

fused to GAL4 DNA-binding domain

mod(mdg4) 
su(Hw) 

Su(Hw)

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD; positive control

(Melnikova et al., 2023)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Melnikova et al., 2023)
FBrf0257791-8.Y2H
Reference
Melnikova et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
su(Hw) 

Su(Hw)

bait

fused to GAL4 DNA-binding domain

mod(mdg4) 

Mod(mdg4)

prey

fused to GAL4 activation domain

mod(mdg4) 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD; positive control

(Melnikova et al., 2023)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Melnikova et al., 2023)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (22)