FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
α-Cat, arm
FlyBase ID
FBig0000078188
Interaction Network
Interactions Browser links
α-Cat network
arm network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0058808-1.coIP.WB
Reference
Oda et al., 1993
Description
physical association
Assay
Collection
Source/Stage
Cell line used
ML-DmBG1
Participants
Corresponds to
Reported as
Role
Note
α-Cat 

Dα-cat

bait
arm 

arm

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of ML-DmBG1 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Oda et al., 1993)
FBrf0058808-2.coIP.WB
Reference
Oda et al., 1993
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 16blastoderm stagegastrula stageembryonic stage 8dorsal closure stageembryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3extended germ band stageembryonic stage 4embryonic cycle 9embryonic stage 2late extended germ band stageembryonic stage 5embryonic stage 11embryonic stage 3embryonic stage 12embryonic stage 15embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic stage 13embryonic cycle 4embryonic stage 14embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
α-Cat 

Dα-cat

bait
arm 

arm

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Oda et al., 1993)
FBrf0058828-1.coIP.WB
Reference
Peifer, 1993
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
arm 

arm

bait
α-Cat 

α-catenin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Peifer, 1993)
FBrf0076120-4.coIP.WB
Reference
Oda et al., 1994
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 16blastoderm stagegastrula stageembryonic stage 8dorsal closure stageembryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3extended germ band stageembryonic stage 4embryonic cycle 9embryonic stage 2late extended germ band stageembryonic stage 5embryonic stage 11embryonic stage 3embryonic stage 12embryonic stage 15embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic stage 13embryonic cycle 4embryonic stage 14embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
α-Cat 

DCAT

bait
arm 

Arm

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Oda et al., 1994)
FBrf0090729-1.coIP.WB
Reference
Orsulic and Peifer, 1996
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
arm 

Arm

bait

Myc tag

arm 
α-Cat 

α-catenin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
arm 
N-terminal region
necessary binding region
aa 101-139

\'S14\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Orsulic and Peifer, 1996)
FBrf0091133-1.PD.WB
Reference
Pai et al., 1996
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 17blastoderm stagegastrula stageembryonic stage 8dorsal closure stageembryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3extended germ band stageembryonic stage 4embryonic cycle 9embryonic stage 2late extended germ band stageembryonic stage 5embryonic stage 11embryonic stage 3embryonic stage 12embryonic stage 15embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic stage 13embryonic cycle 4embryonic stage 14embryonic stage 10embryonic stage 7embryonic stage 16
Cell line used
Participants
Corresponds to
Reported as
Role
Note
arm 

Arm

bait

GST tag

arm 
arm 
arm 
α-Cat 

α-cat

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
arm 
N-terminal region
sufficient binding region
aa 90-165
arm 
N-terminal region
mutation disrupting interaction
aa 148-155aa 132-135aa 98-103aa 94-96

I148A, D152A, D153A, E155A (\'M1\')

R132A, E135A (\'M2\')

R98A, R101A, R103A (\'M3\')

L94A, S95A, Q96A (\'M7\')

arm 
N-terminal region
mutation decreasing interaction
aa 124-126

P124A, Q125A, Q126A (\'M4\')

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Pai et al., 1996)
FBrf0091133-2.FW.WB
Reference
Pai et al., 1996
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 17blastoderm stagegastrula stageembryonic stage 8dorsal closure stageembryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3extended germ band stageembryonic stage 4embryonic cycle 9embryonic stage 2late extended germ band stageembryonic stage 5embryonic stage 11embryonic stage 3embryonic stage 12embryonic stage 15embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic stage 13embryonic cycle 4embryonic stage 14embryonic stage 10embryonic stage 7embryonic stage 16
Cell line used
Participants
Corresponds to
Reported as
Role
Note
arm 

Arm

bait

GST tag

arm 
α-Cat 

α-cat

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
arm 
N-terminal region
sufficient binding region
aa 90-165
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

GST-arm was separated by SDS-PAGE then transferred to nitrocellose. The blot was incubated with protein extract, and bound α-Cat was detected by western blot.

(Pai et al., 1996)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Pai et al., 1996)
FBrf0091133-3.Y2H
Reference
Pai et al., 1996
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
α-Cat 

α-catenin

bait

fused to LexA DNA-binding domain

α-Cat 
arm 

Arm

prey

fused to GAL4 activation domain

arm 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
arm 
N-terminal region
sufficient binding region
aa 90-165
α-Cat 
N-terminal region
sufficient binding region
aa 1-258
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Pai et al., 1996)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Pai et al., 1996)
FBrf0091133-4.Y2H
Reference
Pai et al., 1996
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
arm 

Arm

bait

fused to LexA DNA-binding domain

arm 
α-Cat 

α-catenin

prey

fused to GAL4 activation domain

α-Cat 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
arm 
N-terminal region
sufficient binding region
aa 90-165
α-Cat 
N-terminal region
sufficient binding region
aa 1-258
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Pai et al., 1996)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Pai et al., 1996)
FBrf0091133-6.coIP.WB
Reference
Pai et al., 1996
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
arm 

Arm

bait

Myc tag

arm 
α-Cat 

α-cat

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
arm 
N-terminal region
sufficient binding region
aa 25-165
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Pai et al., 1996)
FBrf0091133-7.PD.WB
Reference
Pai et al., 1996
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 17blastoderm stagegastrula stageembryonic stage 8dorsal closure stageembryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3extended germ band stageembryonic stage 4embryonic cycle 9embryonic stage 2late extended germ band stageembryonic stage 5embryonic stage 11embryonic stage 3embryonic stage 12embryonic stage 15embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic stage 13embryonic cycle 4embryonic stage 14embryonic stage 10embryonic stage 7embryonic stage 16
Cell line used
Participants
Corresponds to
Reported as
Role
Note
α-Cat 

α-cat

bait

GST tag

α-Cat 
arm 

Arm

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
α-Cat 
N-terminal region
sufficient binding region
aa 1-258
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Pai et al., 1996)
FBrf0107664-2.coIP.WB
Reference
Cox et al., 1999
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
arm 

Arm

bait

MYC tag

α-Cat 

α-cat

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Cox et al., 1999)
FBrf0151440-3.coIP.WB
Reference
Geisbrecht and Montell, 2002
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
α-Cat 

&agr-catenin

bait
arm 

Arm

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Geisbrecht and Montell, 2002)

Positive control.

(Geisbrecht and Montell, 2002)
FBrf0208505-3.coIP.WB
Reference
Sawyer et al., 2009
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
arm 

Arm

bait
α-Cat 

αcat

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Sawyer et al., 2009)
FBrf0218395-1016.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
arm 
unspecified role
arm 
unspecified role
arm 
unspecified role
α-Cat 
unspecified role
α-Cat 
unspecified role
α-Cat 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 81.32648

FBrf0220907-1.coIP.WB
Reference
Desai et al., 2013
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
α-Cat 

αCat

bait

HA tag

α-Cat 
α-Cat 
arm 

Arm

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
α-Cat 
vinculin-homology region 1
necessary binding region
aa 1-233
α-Cat 
vinculin-homology region 2
necessary binding region
aa 398-509
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Desai et al., 2013)
FBrf0227798-1.coIP.WB
Reference
Escobar et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
α-Cat 

α-Cat

bait

HA tag

α-Cat 
arm 

Arm

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
α-Cat 
VH1
necessary binding region
aa 1-233
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Escobar et al., 2015)

Deletion of the α-Cat "P-linker", and mutation of phosphorylation sites in this region, don\'t affect the interaction with arm.

(Escobar et al., 2015)
FBrf0228403-2.coIP.WB
Reference
Houssin et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
arm 

Arm

bait
α-Cat 

α-Cat

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

(Houssin et al., 2015)

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Houssin et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (12)