FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Su(dx), Ndfip
FlyBase ID
FBig0000079877
Interaction Network
Interactions Browser links
Su(dx) network
Ndfip network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0214123-3.coIP.WB
Reference
Dalton et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Schneider's-line-2
Participants
Corresponds to
Reported as
Role
Note
Ndfip 

dNdfip

bait

HA tag

Su(dx) 

Su(dx)

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0214123-4.coIP.WB
Reference
Dalton et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Schneider's-line-2
Participants
Corresponds to
Reported as
Role
Note
Su(dx) 

Su(dx)

bait

FLAG tag

Ndfip 

dNdfip

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0240436-1.PD.MW
Reference
Yao et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ndfip 

dNdfip

bait

GST tag

Su(dx) 

Su(dx)

prey

MBP tag

Su(dx) 
Su(dx) 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Su(dx) 
WW12L domains
sufficient binding region
aa 180-466
Su(dx) 
LWW34 domains
sufficient binding region
aa 418-566
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Yao et al., 2018)
FBrf0240436-3.ITC
Reference
Yao et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Su(dx) 

Su(dx)

bait
Su(dx) 
Su(dx) 
Ndfip 

dNdfip

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Su(dx) 
WW12L34 domains
sufficient binding region
aa 180-566

2:1 Ndfip :Su(dx) stoichiometry

Su(dx) 
LWW34 domains
sufficient binding region
aa 418-566

1:1 stoichiometry

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Yao et al., 2018)
FBrf0240436-4.PD.MW
Reference
Yao et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Su(dx) 

Su(dx)

bait

MBP tag

Su(dx) 
Su(dx) 
Ndfip 

dNdfip

prey

Trx or GST tag

Ndfip 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Su(dx) 
WW12L34HECT domains
sufficient binding region
aa 360-949

2:1 Ndfip :Su(dx) stoichiometry

Su(dx) 
WW3 domain
mutation decreasing interaction
aa 489-511

deletion of WW3 domain results in 1:1 stoichiometry

Ndfip 
PY domains
sufficient binding region
aa 21-120
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Yao et al., 2018)

Addition of N can compete with Ndfip for Su(dx) binding when present in large excess.

(Yao et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)