FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000085871
Interaction Network
Interactions Browser links
Ada2a network
Mocs2B network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0204126-12.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Ada2a

bait

TAP tag

CG10238

prey

19 unique peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed in stably transfected cell lines.

Prey endogenous to nuclear extract.

FBrf0204126-15.CH.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Ada2a

neutral component

TAP tag

Ada2a

neutral component

TAP tag

Ada2a

neutral component

TAP tag

CG10238

neutral component

CG10238

neutral component

CG10238

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Proteins derived from affinity-purified TAP-tagged Ada2a complexes in nuclear extract.

Mocs2 coimmunoprecipitates and cofractionates with Ada2a in size exclusion chromatography as part of 1.3-1.8 MDa ATAC histone acetyltransferase complex.

FBrf0204126-26.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

CG10238

bait

HA tag, FLAG tag

Ada2a

prey

4 unique peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed in stably transfected cell lines.

Prey endogenous to embryonic nuclear extract.

FBrf0214172-4.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

CG10238

bait

FLAG tag, HA tag

Ada2a

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
C-terminal region
sufficient binding region
aa 137-367
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Tandem affinity purification of bait and identification of prey by MudPIT.

FBrf0214172-41.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Ada2a

bait

FLAG tag, HA tag

CG10238

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Tandem affinity purification of bait and identification of prey by MudPIT.

FBrf0214172-81.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

CG10238

bait

FLAG tag, HA tag

Ada2a

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
C-terminal region
sufficient binding region
aa 137-367
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0218284-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Ada2a

bait

dMoaE

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait endogenous to nuclear extract.

Prey endogenous to nuclear extract.

FBrf0218284-7.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Ada2a

bait

TAP tag

dMoaE

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Tagged complexes were isolated by affinity purification and separated by size exclusion chromatography before mass spectrometry.

Interact as part of the ATAC histone acetyltransferase complex.

Bait expressed in stably transfected cells.

Prey endogenous to nuclear extract.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)