Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

V12 form of Cdc42 used to detect Cdc42-slpr interaction.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

The Cdc42-slpr interaction was stronger with the GTP bound form of Cdc42.

Cloned reagents; interaction in solution.

Showed binding of Cdc42-GTP at higher level than Cdc42-GDP.